인삼모상근의 생장과 Ginsenosides 생산에 미치는 NaCl의 영향
The Effect of NaCl on the Growth and Ginsenoside Production from Ginseng Hairy Root
2
조회
0
다운로드
6
인용
0
참고문헌
키워드
초록
Korean ginseng (Panax ginseng C.A. Meyer) is very difficult to obtain stable production of qualified ginseng roots because of variable stresses in soil environments. High salt concentrations in the ginseng nursery soil environment of Korea is one of important reducing factors for the stable production of quality ginseng. These studies were accomplished to identify the growth rate and production of ginsenoside from ginseng hairy root against NaCl. In the MS liquid culture, the highest contents and productivity of ginsenosides were appeared at 4 week after onset of the treatment of 0.1M NaCl. And 0.24 M NaCl was more effective on the growth of ginseng hairy root under light condition than dark condition. Plants generally produce secondary metabolites in nature as a defense mechanism against pathogenic and insect attack. In this study, NaCl acts as a kind of stress as well as elicitor for production of ginsenosides.
Abstract
Korean ginseng (Panax ginseng C.A. Meyer) is very difficult to obtain stable production of qualified ginseng roots because of variable stresses in soil environments. High salt concentrations in the ginseng nursery soil environment of Korea is one of important reducing factors for the stable production of quality ginseng. These studies were accomplished to identify the growth rate and production of ginsenoside from ginseng hairy root against NaCl. In the MS liquid culture, the highest contents and productivity of ginsenosides were appeared at 4 week after onset of the treatment of 0.1M NaCl. And 0.24 M NaCl was more effective on the growth of ginseng hairy root under light condition than dark condition. Plants generally produce secondary metabolites in nature as a defense mechanism against pathogenic and insect attack. In this study, NaCl acts as a kind of stress as well as elicitor for production of ginsenosides.
참고문헌 (46)
- Ando T, "Chemical studies on the oriental plant drugs. Comparative studies on the saponins and sapoginins of ginseng and related crude drugs", Syoyakugaku Zasshi, 25, pp.28~32 (1971)
- Arya S, "Plant regeneration from protoplasts of Panax ginseng (C.A. Meyer) through somatic embryogenesis", Plant Cell Rep, 10, pp.277~281 (1991)
- Asins MJ, "Salt tolerance in Lycopersicon species. I. Character definition and changes in gene expression", Theor. Appl. Genet, 86, pp.737~743 (1993)
- Bhagwath SG, "Statigical analysis of elicitation strategies for thiarubrine: A production in hairy root cultures of Ambrosia artemisiifolia", J. Biotechnol, 80, pp.159~167 (2000)
- Boyer JS, "Plant productivity and environment", Science, 218, pp.443~448 (1982)
- Brachet J, "Changes in the total alkaloids content of Datura innoxia Mill subjected to salt stress", J. Exp. Bot, 37, pp.650~656 (1986)
- Butenko RG, "Organogenesis and embryogenesis in a tissue culture(Panax ginseng) and other Panax L", Species. Bot. Zh, 53(7), pp.906~911 (1968)
- Chae YA, "In vitro breeding for salttolerance rice: Callus growth in stepwise increase of saline stress and the nature of salt tolerance", Korea J. Breeding, 21, pp.46~50 (1988)
- Croughan SF, "Selection of a NaCl tolerant line of cultured alfalfa cell", Crop Sci, 18, pp.959~963 (1978)
- Dicosmo F, "Eliciting secondary metabolism in plnat cell cultures", Trends Biotechnol, 3, pp.318~322 (1985)
- Frischlnecht PM, "Stress-induced formation of purine alkaloids in plant tissue cultures of Coffea arabica", Phytochem, 24, pp.2255~2257 (1985)
- Hans DV, "Two classes of plant antibiotics: phytoalexins versus phytoantipicins", The Plant Cell, 6, pp.1191~1192 (1994)
- Hiraga H, "Angiotensin I-converting enzyme gene polymorphism and salt sensitivity in essential hypertension", Hypertension, 27, pp.69~572 (1996)
- Hong SJ, "Biosynthesis of saponins in Panax ginseng", Kor. J. Ginseng Sci, 11, pp.136~144 (1987)
- Huraki C, "Detection of methicillin-resistant Staphylococcus aureus using PCR and non-radioactive DNA proves", Rinsho Byori, 41, pp.1159~1166 (1993)
- 인준교, "염류내성관련 SAL1 유전자에 의한 인삼 형질전환", 한국약용작물학회지, 13(1), pp.57~62 (2005), 한국약용작물학회
- 양덕춘, "인삼모상근의 생장과 Ginsenoside 생합성에 미치는 KH2PO4의 영향", 한국약용작물학회지, 14(6), pp.371~375 (2006), 한국약용작물학회
- Don-Hee Park, "Enhancement of Growth and Secondary Metabolite Biosynthesis: Effect of Elicitors Derived from Plants and Insects", Biotechnology and Bioprocess Engineering, 10(1), pp.73~77 (2005), 한국생물공학회
- Jeong GT, "Enhanced secondary metabolite biosynthesis by elicitation in transformed plant root system: effect of abiotic elicitors", Appl. Biochem. Biotechnol, 129(132), pp.436~446 (2006)
- Jolley KA, "Dihydrolipoamide dehydrogenase from the halophilicarchaeon Haloferax volcanii : homology over expression of the cloned gene", J. Bacteriol, 178, pp.3044~3048 (1996)
- Joo CN, "Biosynthesis of saponins in Panax ginseng C. A. Meyer. I. Probable sites of the biosynthesis of ginseng saponin from acetate", Kor. J. Ginseng Sci, 7, pp.108~114 (2000)
- Jung G, "Use of genetic transformation by the Ri T-DNA of Agrobacterium rhizogenes to stimulate bio-mass and tropane alkaloid production in Atopa belladonna and Calystegia Sepium roots grown in vitro", Plant Sci, 50, pp.145~151 (1987)
- 정희영, "미치광이풀 모상근 배양에서 Tropane Alkaloids 생산성 증진을 위한 최적 생물학적 엘리시터 선발", 한국약용작물학회지, 11(5), pp.358~363 (2003), 한국약용작물학회
- Kent FM, "Salt-inducible betaine aldehyde dehydrogenase from sugar beet: cDNA cloning and expression", Plant Mol. Biol, 18, pp.1~11 (1992)
- Leon DD, "Rapid in vitro screening of some salt tolerant bread wheats", Cereal Research Communications, 23(4), pp.383~389 (1995)
- Murashige T, "A revised medium for rapid growth and bioassays with tobacco tissue culture", Plant Physiol, 15, pp.473~497 (1962)
- Nabors MW, "Sodium chloride tolerant lines of tobacco cells", Plant Sci. Lett, 4, pp.155~159 (1975)
- Park HJ, "Effect of jasmonic and methyl jasmonate on the production of ginsenosides in the hairy roots of Korean ginseng (Panax ginseng C.A. Meyer)", J. Ginseng Res, 24, pp.74~78 (2000)
- Ryu JH, "Tissue culture for the selection salt tolerant lines in Alfalfa (Medicago sativa L.)", Kor. J. Plant Tiss. Cult, 4, pp.239~246 (1991)
- Sim SJ, "Production and secretion of indole alkaloids in hairy root cultures of Catharan thus roseus: eects of in situ adsorption, fungal elicitation and permeabilization", J. Ferment. Bioeng, 78, pp.229~234 (1994)
- Smith JI, "The use of organic compounds to increase the accumulation of indole alkaloids in Catharanthus reseus (L.) G. Cell suspension cultures", J. Exp. Bot, 38, pp.1501~1506 (1987)
- Vasil V, "Sormatic embryogenesis and plant regeneration from suspended cultures of Pearl millet", Ann. Sot, 47, pp.669~678 (1981)
- Yang DC, "Selection of active grow hairy root lines in ginseng", Kor. J. Plant Tiss. Cult, 25(6), pp.525~530 (1998)
- Yoon YS, "Selection of ginseng superior lines tolerant to salt stress through zygotic embryo culture", Kor. J. Plant Res, 17(3), pp.257~264 (2004)
- You BS, "Characterization of batch culture and effect of various elicitors on ginsenoside production in suspension cultures of Panax ginseng C.A. Meyer", Kor. J. Biotechnol. Bioeng, 16, pp.620~625 (2001)
- 남기열, "토양염류 농도가 고려인삼의 생육 및 수량에 미치 는 영향", 박사, pp.1991
- 남기열, "최신고려인삼-성분 및 효능편. 한국인삼연초연구원", pp.13~44 (1996), 천일인쇄사
- 인삼의 생리장해에 관한 연 구, "목성균", 한국인삼연초연구소, 353~493 (1987)
- 고려인삼, "배효원", 고려인삼연구소, 238~238 (1979)
- 인삼토양의 이화학성개량연구, 인삼연구보고서(재배분야), "송기준", 인 삼연초연구소, 681~783 (1985)
- 양춘덕, "인삼 염류내성 계통의 선발을 위한 배배양 배지조건", 한국약용작물학회지, 11(2), pp.162~166 (2003), 한국약용작물학회
- 오승용, "Chitin과 Chitosan 처리에 의한 인삼모상근으로부터 Ginsenosides 생산", 고려인삼학회지, 24(2), pp.68~73 (2000)
- 윤영상, "염류내성관련 유전자 Betaine Aldehyde Dehydrogenase Gene의 인삼 체세포 배발생을 통한 형질전환", 한국자원식물학회지, 18(1), pp.15~21 (2005), 한국자원식물학회
- 이태수, "적변인삼의 화학적 성분에 관 한 연구", 고려인삼학회지, 19, pp.77~83 (1995)
- 양덕춘, "광 및 UV 조사가 인삼 모상근의 생장 및 사포닌 생합성에 미치는 영향", 한국약용작물학회지, 14(6), pp.360~366 (2006), 한국약용작물학회
- 정현숙, "벼 callus의 유도와 성장에 미치는 염의 영향", Kor. J. Plant Tiss. Cult, 9(1), pp.35~42 (1982)
