기내배양을 통한 고려인삼(Panax ginseng C.A. Meyer)조직의 NaCl에 대한 반응
In vitro Culture Response to NaCl of Korean Ginseng(Panax ginseng C.A. Meyer) Tissues
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인삼포장에서 염류의 집적은 우량인삼의 생산에 많은 장애요인이 되고 있다. 본 연구는 인삼종자의 NaCl에 대한 반응정도를 조사하고, 또한 체세포배의 발생시 및 인삼모상근에서 사포닌의 생성에 미치는 반응을 조사하였다. 인삼 접합자 배를 NaCl이 첨가되지 않은 배지에 접종한 결과 84.5%가 발아되었으며 0.1M에서는 3%만이 발아되었고, 0.2M이상의 처리구에서는 전혀 발아가 되지 않았다. 또한 체세포배 발생은 미숙배를 이용할 경우 NaCl 무첨가배지에서 가장 양호하였으며 농도가 증가할수록 감소하는 경향을 보였다. 반면에 성숙배에서는 NaCl 무 첨가배지보다 0.05M첨가배지에서 더 양호한 경향을 보였다. 뿌리 상태인 인삼의 모상근의 경우에는 광상태에서 NaCl를 첨가한 배지에 배양시 0.04M에서부터 안토시안이 형성되기 시작하여 0.08M에서는 눈으로 확인이 가능할 정도의 많은 량이 형성되었다. 안토시안을 함유한 세포주는 다른 세포주에 비해 crude saponin뿐만 아니라 total ginsenoside의 함량 도 높 은 경 향 을 보 였 으 며 panaxatriol보 다 panaxadiol이 2배이상 많았다.
Abstract
High salt concentrations in the ginseng nursery soil environment of Korea is one ofimportant reducing factors for the stable production of quality ginseng. These studies wereaccomplished for check the response on germination of ginseng seed, somatic embryogenesisof zygotic embryo, and biosynthesis of ginsenoside from ginseng hairy root against NaCl.Ratio of germination was at the 3% and 84.5% on the basic media with 0.1M and free ofNaCl repectedly, but 0% at the upper of 0.2M NaCl. Somatic embryogenesis from zygoticembryo were the highest when immatured embryo was cultured on free of NaClconcentration, and which was intend to decrease at treatment of NaCl. However, in case ofusing the matured embryo, treatment of 0.05M NaCl resulted in better embryogenesis thanNaCl free media. Red pigment was synthesized from ginseng hairy root cultured on themedium with various NaCl concentration(from 0.04 to 0.08M) and its pigment was analyzedas spectrum of anthocyane by spectrophoto- meter scanning. This cell line biosynthesizedlots of crude saponin and total ginsenoside than other cell lines, also had 2 times ofpanaxadiol than panaxatriol.
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