Methy1 jasmonate 처리에 의한 인삼 (Panax ginseng C.A Meyer) 부정근의 이차대사산물 및 항산화활성 증가
Increasement of Secondary Metabolites and Antioxidative Activity in Panax ginseng Adventitious Root by methyl Jasmonate
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인삼부정근 배양에서 MeJA 처리가 부정근내 진세노사이드와 페놀화합물의 생산에 미치는 영향과 이러한 이차대사산물의 증가에 따른 인삼부정근의 항산화활성의 효과를 조사하였다. 다양한 농도의 MeJA를 인삼부정근에 처리한 결과, 100 μM MeJA에서 부정근내 진세노사이드의 생산은 26.6 mg/g dry wt로 대조구보다 약 8배 이상 생산하는 것으로 나타났다. 하지만, MeJA의 처리는 부정근의 생장을 감 소시키는 것으로 나타났다. 페놀화합물의 생산 역시 MeJA 처리에 의하여 증가되는 경향을 나타냈으며 50 μM MeJA에서 0.38 mg/g dry wt로 대조구에 비하여 약 3배 이상 증가한 것으로 나타났다. 이러한 MeJA의 처리효과는 조건배 지 (CM)를 이용하는 것이 신선배지 (FM)를 이용하는 것보다 유리한 것으로 나타났다. 배지성분이 결핍된 조건배지에서의 진세노사이드 생산은 신선배지에서 보다 약 1.7배가 증가한 것으로 나타났으며, 페놀화합물 역시 조건배지에서 약 1.2배가 증가한 것으로 나타났다. 이러한 MeJA 처리에 의한 진세노사이드와 페놀화합물의 증가는 결과적으로 인삼부정근의 항상화활성을 무처리구인 대조구에 비해서 약 72%이상 증가시키는 것으로 나타났다.
Abstract
This study was initiated to investigate the impacts of methyl jasmonate (MeJA) on adventitious rootgrowth of Panax ginseng, the production of secondary metabolites, such as ginsenosides and phenolic compounds, and antioxidative activity. Among various concentrations of MeJA, 100 μM MeJA increased the ginsenosides accumulation to 26.6 mg/g dry wt, about 8 times higher than the control in ginseng adventitious roots (GAR). In addition, 50 μM MeJA increased the accumulation of phenolic compounds to 0.38 mg/g dry wt, about 3 times higher than control in GAR. This Me(CM) which obtained in bioreactor after 40 days of culture than in fresh medium (FM). Treatment of 100 M MeJA in CM increased the accumulation of ginsenosides (1.7 times) and phenolic compounds (1.2 times) more than in FM, respectively. Consequently, these high accumulation of ginsenosides and phenolic compounds by MeJA led to increase the antioxidative activities expressed to the DPPH scavenging activity (over 78.3%). The DPPH scavenging activity in control was 45.5%.
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