CPAE 세포를 이용한 Kaempferol과 Quercetin의 산화스트레스 극복효과
Protective Effects of Kaempferol andQuercetin onOxidative Stress in CPAE Cell
0
조회
0
다운로드
3
인용
0
참고문헌
키워드
초록
Flavonoids are ubiquitous substances in fruits and vegetables. A main subgroup of the flavonoids are the flavonols, of which quercetin and kaempferol are the major representatives in foods. They are used in food supplements at high doses, because of their preventive effects on degenerative diseases. The aim of this study was to determine the combined and separate effects of kaempferol and quercetin on oxidative stress in cow pulmonary artery endothelium (CPAE) cells over a broad concentration range. The results demonstrate that cell viability was greatly increased in kaempferol and quercetin treated cells whether H2O2-treated or not. Cell viability also increased when treated with flavonols in the absence of oxidative stress. Both preincubation and simultaneous incubation with kaempferol and quercetin protected against the loss of cell viability induced by 1mM H2O2(5h). Protective effects of flavonols against oxidative stress were shown to depend on the treated flavonol concentrations. No protective effect was shown under low concentration treatment and cell viability increased 1.6 times at 200μMrelative to the control group. At the highest flavonol concentration of 300μM, the increased cell viability by flavonol treatment was decreased to almost half of the maximum values. Combined treatments with kaempferol and quercetin showed more protective effects against oxidative stress by H2O2 than the separate effects of each flavonol. In conclusion, the protective effect of kaempferol and quercetin against oxidative stress was very pronounced but high concentrations of flavonols can also induce cell cytotoxicity.
Abstract
Flavonoids are ubiquitous substances in fruits and vegetables. A main subgroup of the flavonoids are the flavonols, of which quercetin and kaempferol are the major representatives in foods. They are used in food supplements at high doses, because of their preventive effects on degenerative diseases. The aim of this study was to determine the combined and separate effects of kaempferol and quercetin on oxidative stress in cow pulmonary artery endothelium (CPAE) cells over a broad concentration range. The results demonstrate that cell viability was greatly increased in kaempferol and quercetin treated cells whether H2O2-treated or not. Cell viability also increased when treated with flavonols in the absence of oxidative stress. Both preincubation and simultaneous incubation with kaempferol and quercetin protected against the loss of cell viability induced by 1mM H2O2(5h). Protective effects of flavonols against oxidative stress were shown to depend on the treated flavonol concentrations. No protective effect was shown under low concentration treatment and cell viability increased 1.6 times at 200μMrelative to the control group. At the highest flavonol concentration of 300μM, the increased cell viability by flavonol treatment was decreased to almost half of the maximum values. Combined treatments with kaempferol and quercetin showed more protective effects against oxidative stress by H2O2 than the separate effects of each flavonol. In conclusion, the protective effect of kaempferol and quercetin against oxidative stress was very pronounced but high concentrations of flavonols can also induce cell cytotoxicity.
참고문헌 (18)
- Ackland,M.L., "Synergistic antiproliferative action of the flavonols quercetin and kaempferol in cultured human cancer cell lines", In Vivo, 19, pp.69~76 (2005)
- Di Carlo, "Falvonoids: old and new aspects of a class of natural therapeutic drugs", Life Sci, 65, pp.337~353 (1999)
- Fang Y.Z., "Free radicals, antioxidants, and nutrition", Nutrition, 18, pp.872~879 (1996)
- Gordon,M.H., "Dietary antioxidants in disease prevention", 265~273 (1996)
- Hollman,P.C.H., "Role of dietary flavonoids in protection against cancer and coronary heart disease", Bioact. Compd. Food, 24, pp.785~789 (1996)
- Jang,J.H., "Protective effects of resveratrol on hydrogen peroxide-induced apoptosis in rat pheochromocytoma (PC 12)cells", Mutat. Res, 496, pp.181~190 (2001)
- Jayaprakasha,G.K., "Antioxidant activity of grape seed(Vitis vinifera)extracts on peroxidantion models in vitro", Food Chem, 73, pp.285~290 (2001)
- Juana, B., "Antioxidant properties and protective effects of a standardized extract of Hypericum perforatum on hydrogen peroxide-induced oxidative damage in PC12 cells", Life Sci, 75, pp.1263~1276 (2004)
- Kenkt,P., "Flavonoid intake and risk of chronic diseases", Am. J. Clin. Nutr, 76, pp.560~568 (2002)
- Kenkt, P., "Flavonoid intake and risk of chronic diseases", Am. J. Clin. Nutr, 76, pp.560~568 (2002)
- Kuhn,M.A, "Oxygen free radicals and antioxidants", Am. J. Nurs, 103, pp.58~62 (2003)
- 이상은, "산화적 스트레스에 대한 섬오갈피 메탄올 추출물의 보호 효과", 생약학회지, 37(1), pp.16~20 (2006), 한국생약학회
- Maria,C., "Direct HPLC analysis of quercetin and transresveratrol in red wine byproducts", J. Agric. Food Chem, 51, pp.5226~5231 (2003)
- Nordmann, R., "Free radicals, oxidative stress and antioxidant vitamins", C.R. Seances Soc Biol Fil, 187, pp.277~285 (2005)
- Park,S.Y., "Antioxidant activity of common fruits and vegetables", J. Cheju Halla College, 29, pp.59~67 (2005)
- Selloum, L., "Effects of flavonols on the generation of superoxide anion radicals by xanthine oxidase and stimulated neutrophils", Arch. Biochem. Biophys, 395, pp.49~56 (2001)
- Takeo,E., "Sweet elements of Siraitia Grosvenori inhibit oxidative modification of low-density lipoprotein", J. Atherosclerosis and Thrombosis, 9, pp.114~120 (2001)
- Temple,N.J., "Antioxidants and disease: more question than answers", Nutrition Research, 20, pp.449~459 (2000)