오레가노(Origanum majorana L.) 추출물의 항산화 효과
he Antioxidative Effects of Oregano (Origanum majorana L.) Extracts
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본 연구의 목적은 오레가노 추출물의 유리 라디칼 소거 및 지질과산화 억제 등 항산화 효과를 조사하기 위함이다. Pyrogallol의 억제율을 100%로 기준하였을 때, DPPH 라디칼을 50% 억제시키는데 필요한 오레가노 함량은 21.8 μg이었다. 총항산화 활성은 강력한 자유라디칼에 대한 총항산화능으로 측정하였다. 오레가노 추출물 7.5 및 15 μg의 총항산화능은 각각 15.1 및 31.4 nmol Trolox와 대등한 수준이었다. 오레가노 추출물 0.2 및 0.4 μg의 산소라디칼 소거능은 각각 1.4 및 2.4 nmol gallic acid와 대등한 수준이었다. 오레가노 추출물 30 및 75 μg의 총페놀 함량은 각각 40.5 및 83.9 nmol gallic acid와 대등한 수준이었다. 지질과산화에 대한 억제효과는 이황화철/아스코르빈산에 의해 유도된 랫드 간 미토콘드리아를 이용하여 조사하였다. 오레가노 추출물 20 및 50 μg은 TBARS 수준을 각각 20 및 64% 억제시켰다. 이와같이 오레가노 추출물의 강력한 항산화 효과는 적어도 자유라디칼의 산화 억제와 지질과산화 억제에 기인하는 것으로 사료된다.
Abstract
The objective of this study was to investigate the antioxidative capacity of oregano extracts. Amount of oregano extract at which DPPH radical scavenging activity was inhibited by 50% was 21.8 μg as compared to 100% by pyrogallol as a reference. Total antioxidant status was examined by total antioxidant capacity against potent free radical reactions. Total antioxidant capacities of oregano extract at the amounts of 7.5 and 15 μg were 15.1 and 31.4 nmol Trolox equivalents, respectively. Oxygen radical absorbance capacities of oregano extract at the amounts of 0.2 and 0.4 μg were 1.4 and 2.4 nmol gallic acid equivalents, respectively. Total phenolic contents of oregano extract at the amounts of 30 and 75 μg were 40.5 and 83.9 nmol gallic acid equivalents, respectively. The inhibitory effect of oregano extract on lipid peroxidation was examined using rat liver mitochondria induced by FeSO4/ascorbic acid. Oregano extracts at the amounts of 20 and 50 μg decreased TBARS level by 20 and 64%, respectively. Thus strong antioxidant effects of oregano extract seem to be due to, at least in part, the prevention against free radicals-induced oxidation, followed by inhibition of lipid peroxidation.
참고문헌 (22)
- Alma, M.H., "Screening chemical composition and in vitro antioxidant and antimicrobial activities of the essential oils from Origanum syriacum L", growing in turkey. Biol. Pharm. Bull., 26, pp.1725~1729 (2003)
- Block,G., "Fruits, vegetables and cancer prevention:a review of the epidemiological evidence", Nutr. Cancer, 18, pp.1~29 (1992)
- Bradford,M.M, "A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding", Anal. Biochem., 72, pp.248~254 (1976)
- Diplock, A.T., "Functional food science and defence against reactive oxidative species", Brit. J. Nutr., 80S, pp.S77~S112 (1998)
- Dorman, H.J.D., "Antioxidant properties of aqueous extracts from selected Lamiaceae species grown in turkey", J. Agric. Food Chem., 52, pp.762~770 (2004)
- Elgayyar, M., "Antimicrobial activity of essential oils from plants against selected pathogenic and saprophytic microorganisms", J. Food Prot., 7, pp.1019~1024 (2001)
- Erel,O, "A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation", Clin. Biochem., 37, pp.277~285 (2004)
- Halliwell,B., "Free radicals, antioxidants and human disease:where are we now?", J. Lab Clin. Med., 119, pp.598~620 (1992)
- Ho-Jin Heo, "Ursolic Acid of Origanum majorana L. Reduces Aβ-induced Oxidative Injury", 분자와 세포, 13(1), pp.5~11 (2002), 한국분자세포생물학회
- Hovius, R., "Improved methods to isolate and subfractionate rat liver mitochondria. Lipid composition of the inner and outer membrane", Biochim. Biophys. Acta., 1021, pp.217~226 (1990)
- Huang, D., "High-throughput assay of oxygen radical absorbance capacity (ORAC) using a multichannel liquid handling system coupled with a microplate fluorescence reader in 96-well format", J. Agric. Food Chem., 50, pp.4437~4444 (2002)
- Jun, W.J., "Antioxidant effects of Origanum majorana L. on superoxide anion radicals", Food Chem., 75, pp.439~444 (2001)
- Lam,L.K.T., "Effets of essential oils of glutathione S-transferase activity in mice", J. Agric. Food Chem., 39, pp.660~662 (1991)
- Malterud,K.E., "Antioxidant and radical scavenging effects of anthraquinones and anthrones", Pharmacology, 47, pp.77~85 (1993)
- Shan, B., "Antioxidant capacity of 26 spice extracts and characterization of their phenolic constituents", J. Agric. Food Chem., 53, pp.7749~7759 (2005)
- Singleton, V.L, "Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent", Methods Enzymol., 299, pp.152~178 (1999)
- Sivropoulou, A., "Antimicrobial and cytotoxic activities of Origanum essential oils", J. Agric. Food Chem., 44, pp.1202~1205 (1996)
- Sokmen, M., "In vitro antioxidant, antimicrobial, and antiviral activities of the essential oil and various extracts from herbal pars and callus cultures of Origanum acutidens", J. Agric. Food Chem., 52, pp.3309~3312 (2004)
- Stacey, N.H, "of lipid peroxidation without prevention of cellular injury in isolated rat hepatocytes", Toxicol. Appl. Pharm., 58, pp.8~18 (1981)
- Steel, R.G.D, "Principles and procedures of statistics, 2nd ed", McGraw-Hill, 186~187 (1980)
- Thannickal,V.J., "Reactive oxygen species in cell signaling", Am. J. Physiol. Lung Cell Mol. Physiol., 279, pp.L1005~L1029 (2000)
- Yoshino,K., "Antioxidant and antiinflammatory activities of Oregano extract", J. Health Sci., 52, pp.169~173 (2006)