소리쟁이 추출물의 항산화 효능
Antioxidative Activity of Rumex crispus L. Extract
1
조회
0
다운로드
15
인용
0
참고문헌
키워드
초록
본 연구에서는 소리쟁이 에탄올추출물의 항산화 효과를조사하였다. Pyrogallol의 억제율을 100%로 기준하였을 때,DPPH 라디칼을 50% 억제시키는데 필요한 소리쟁이 추출물의 농도는 2.15 mg/mL으로 α-tocopherol의 IC50(0.43mg/mL)에 비해 높게 나타났다. 총항산화능은 ABTS 라디칼에 대한 소거활성으로 측정하였다. 소리쟁이 추출물 0.1및 1 mg/mL의 총항산화능은 각각 0.47 및 2.33 mM Trolox와 동등한 수준이었으며, α-tocopherol에 비해 높게나타났다. 소리쟁이 추출물 0.1 및 1 mg/mL의 superoxide 소거활성은 각각 21.5 및 78.9%이었으며, catechin에 비해 차이가 없었다. 소리쟁이 추출물 20 및 100 μg/mL의peroxyl 라디칼 소거활성은 각각 62.5 및 156.4 μM Trolox와 동등한 수준이었으며, ascorbic acid에 비해 낮게 나타났다. 소리쟁이 추출물 0.1 및 1 mg/mL의 구리이온 환원력은 각각 0.28 및 1.88 mM Trolox와 동등한 수준이었으며, α-tocopherol에 비해 유사하거나 높게 나타났다. 소리쟁이 추출물은 hydroxyl 라디칼 및 peroxyl 라디칼로 유발된 supercoiled DNA strand 절단을 억제시켰다. 소리쟁이 추출물 0.5 및 5 mg/mL의 총페놀 함량은 각각 0.58 및 3.85 mM gallic acid와 동등한 수준으로 높게나타났다. 또한, HepG2 세포주를 이용한 세포배양에서 소리쟁이 추출물 0.1 및 0.5 mg/mL 농도의 첨가는 0.2 mM tert-butyl hydroperoxide로 유도된 세포독성을 각각 38.5및 63.5% 감소시켰다. 따라서, 본 연구 결과들은 소리쟁이추출물의 강력한 항산화 효과와 세포독성 억제 효과를 나타내며, 이러한 효능은 적어도 자유라디칼의 산화 억제와높은 총페놀 함량에 기인하는 것으로 사료된다.
Abstract
The objective of this study was to investigate the antioxidative capacity of ethanol extracts from Rumex crispus L. The concentration of R. crispus L. extract at which DPPH radical scavenging activity was inhibited by 50% was 2.15mg/mL, which was lower than that of α-tocopherol (0.43 mg/mL), as compared to 100% by pyrogallol as a reference. Total antioxidant status was examined by total antioxidant capacity against ABTS radical reactions. Total antioxidant capacities of R. crispus L. extract at concentrations of 0.1 and 1 mg/mL were 0.47 and 2.33 mM Trolox equivalents, respectively,which were higher than those of α-tocopherol. Superoxide scavenging activities of R. crispus L. extract at concentrations of 0.1 and 1 mg/mL were 21.5 and 78.9%, respectively, which were not significantly (p>0.05) different from those of catechin. Oxygen radical absorbance capacities of R. crispus L. extract at concentrations of 20 and 100 μg/mL were 62.5 and 156.4 μM μM Trolox equivalents, respectively, which were lower than those of ascorbic acid. Cupric reducing antioxidant capacities of R. crispus L. extract at concentrations of 0.1 and 1 mg/mL were 0.28 and 1.88 mM Trolox equivalents, which were similar or significantly (p<0.05) higher than those of α-tocopherol, respectively. R. crispus L. extract prevented supercoiled DNA strand breakage induced by hydroxyl radical and peroxyl radical. Total phenolic contents of R. crispus L. extract at concentrations of 0.5 and 5 mg/mL were 0.58 and 3.85 mM gallic acid equivalents, respectively. R. crispus L. extract at concentration of 0.1 and 0.5 mg/mL inhibited 0.2 mM tert-butyl hydroperoxide-induced cytotoxicity by 38.5 and 63.5%, respectively, in HepG2 cell culture system. Thus, strong antioxidant and cytotoxicity-inhibiting effects of R. crispus L. extract seem to be due to, at least in part, the prevention from free radicals-induced oxidation as well as high levels in total phenolic contents.
참고문헌 (25)
- Apak, R., "Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method", J. Agric. Food Chem, 52, pp.7970~7981 (2004)
- Bae, K., "The Medical Plants of Korea", pp.92~ (2000), Kyo-Hak Publising Co.
- Erel, O., "A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation", Clin. Biochem, 37, pp.277~285 (2004)
- Günaydin, K., "1,5-Dihydroxyanthraquinones and an anthrone from roots of Rumex crispus", Nat. Prod. Lett, 16, pp.65~70 (2002)
- Halliwell, B., "Free radicals, antioxidants and human disease:where are we now?", J. Lab Clin. Med, 119, pp.598~620 (1992)
- Hiramoto, K., "Effect of plant phenolics on the formation of the spin-adduct of hydroxyl radical and the DNA strand breaking by hydroxyl radical", Biol. Pharm. Bull, 19, pp.558~563 (1996)
- Hu, C., "Evaluation of antioxidant and prooxidant activities of bamboo Phyllostachys nigra var. Henonis leaf extract in vitro", J Agric. Food Chem, 48, pp.3170~3176 (2000)
- 황선우, "참소리쟁이 뿌리에서 안트라퀴논계 화합물의 분리 및 생리활성", Journal of the Korean Society for Applied Biological Chemistry, 47(2), pp.274~278 (2004), 한국응용생명화학회
- Huang, D., "High-throughput assay of oxygen radical absorbance capacity (ORAC) using a multichannel liquid handling system coupled with a microplate fluorescence reader in 96-well format", J. Agric. Food Chem, 50, pp.4437~4444 (2002)
- 박돈희, "소리쟁이 추출물의 항균 및 항산화 활성에 관한 연구", Korean Chemical Engineering Research(HWAHAK KONGHAK), 44(1), pp.81~86 (2006), 한국화학공학회
- Kim, D.K., "Cytotoxic constituents of Rumex japonicus", Yakhak Hoeji, 42, pp.233~237 (1998)
- 김현정, "소리쟁이 추출물의 항산화 활성과 이를 첨가한 양념돈육의 품질특성", 한국식품과학회지, 42(4), pp.445~451 (2010), 한국식품과학회
- 정규영, "Screening extracts of Achyranthes japonica and Rumex crispus for activity against various plant pathogenic fungi and control of powdery mildew", PEST MANAGEMENT SCIENCE, 60, pp.803~808, JOHN WILEY & SONS LTD
- 이숙연, "소리쟁이 종자의 소염진통작용 및 간보호 효과", 생약학회지, 38(4), pp.334~338 (2007), 한국생약학회
- Liu, F., "Free radical scavenging activities of mushroom polysaccharide extracts", Life Sci, 60, pp.763~771 (1997)
- Maksimović, Z., "Antioxidant activity of yellow dock (Rumex crispus L., Polygonaceae) fruit extract", Phytother. Res, 25, pp.101~105 (2011)
- Malterud, K.E., "Antioxidant and radical scavenging effects of anthraquinones and anthrones", Pharmacology, 47, pp.77~85 (1993)
- Mosmann, T., "Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays", J. Immunol. Methods, 65, pp.55~63 (1983)
- 임태진, "개머루덩굴 추출물의 항산화 효과", 한국자원식물학회지, 23(5), pp.445~450 (2010), 한국자원식물학회
- 임태진, "만병초(Rhododendron brachycarpum) 추출물의 항산화 효과", 한국자원식물학회지, 24(4), pp.456~460 (2011), 한국자원식물학회
- Singleton, V.L., "Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent", Methods Enzymol, 299, pp.152~178 (1999)
- Steel, R.G.D., "Principles and Procedures of Statistics, 2nd ed", pp.186~187 (1980), McGraw-Hill
- Suh, H.J., "Determination of singlet oxygen quenching and protection of biological systems by various extracts from seed of Rumex crispus L", J. Photochem. Photobiol. B, 102, pp.102~107 (2011)
- Thannickal, V.J., "Reactive oxygen species in cell signaling", American J. Physiol. Lung Cell Mol. Physiol, 279, pp.L1005~L1029 (2000)
- Yildirim, A., "Determination of antioxidant and antimicrobial activities of Rumex crispus L. extracts", J. Agric. Food Chem, 49, pp.4083~4089 (2001)