Genetic Diversity and Population Structure of Korean Mint Agastache rugosa (Fisch & Meyer) Kuntze (Lamiaceae) Using ISSR Markers
0
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Agastache rugosa, a member of the mint family (Labiatae), is a perennial herb widely distributed in East Asiancountries. It is used in traditional medicine for the treatment of cholera, vomiting, and miasma. This study assessed thegenetic diversity and population structures on 65 accessions of Korean mint A. rugosa germplasm based on inter simplesequence repeat (ISSR) markers. The selected nine ISSR primers produced reproducible polymorphic banding patterns. Intotal, 126 bands were scored; 119 (94.4%) were polymorphic. The number of bands generated per primer varied from 7 to18. A minimum of seven bands was generated by primer 874, while a maximum of 18 bands was generated by the primer844. Six primers (815, 826, 835, 844, 868, and 874) generated 100% polymorphic bands. This was supported by otherparameters such as total gene diversity (HT) values, which ranged from 0.112 to 0.330 with a mean of 0.218. The effectivenumber of alleles (NE) ranged from 1.174 to 1.486 with a mean value of 1.351. Nei’s genetic diversity (H) mean value was0.218, and Shannon’s information index (I) mean value was 0.343. The high values for total gene diversity, effectivenumber of alleles, Nei’s genetic diversity, and Shannon’s information index indicated substantial variations within thepopulation. Cluster analysis showed characteristic grouping, which is not in accordance with their geographical affiliation. The implications of the results of this study in developing a strategy for the conservation and breeding of A. rugosa and othermedicinal plant germplasm are discussed.
Abstract
Agastache rugosa, a member of the mint family (Labiatae), is a perennial herb widely distributed in East Asian countries. It is used in traditional medicine for the treatment of cholera, vomiting, and miasma. This study assessed the genetic diversity and population structures on 65 accessions of Korean mint A. rugosa germplasm based on inter simple sequence repeat (ISSR) markers. The selected nine ISSR primers produced reproducible polymorphic banding patterns. In total, 126 bands were scored; 119 (94.4%) were polymorphic. The number of bands generated per primer varied from 7 to 18. A minimum of seven bands was generated by primer 874, while a maximum of 18 bands was generated by the primer 844. Six primers (815, 826, 835, 844, 868, and 874) generated 100% polymorphic bands. This was supported by other parameters such as total gene diversity (HT) values, which ranged from 0.112 to 0.330 with a mean of 0.218. The effective number of alleles (NE) ranged from 1.174 to 1.486 with a mean value of 1.351. Nei’s genetic diversity (H) mean value was 0.218, and Shannon’s information index (I) mean value was 0.343. The high values for total gene diversity, effective number of alleles, Nei’s genetic diversity, and Shannon’s information index indicated substantial variations within the population. Cluster analysis showed characteristic grouping, which is not in accordance with their geographical affiliation. The implications of the results of this study in developing a strategy for the conservation and breeding of A. rugosa and other medicinal plant germplasm are discussed.
참고문헌 (38)
- Bernath, J,, "Conventional breeding methods and their effectiveness in selection of medicinal and aromatic plants", In Proceedings of the First International Symposium on Breeding Research on Medicinal and Aromatic Plants, Quedlinburg, pp.154~161 (1996)
- Bernath, J, "Strategies and recent achievements in selection of medicinal and aromatic plants", Acta. Hortic, 576, pp.65~68 (2002)
- Bornet, B., "Nonanchored inter simple sequence repeat(ISSR)markers : reproducible and specific tools for genome fingerprinting", Plant Mol. Biol. Rep, 19, pp.209~215 (2001)
- Bornet, B., "Genetic diversity in European and Argentinian cultivated potatoes(Solanum tuberosum subsp. tuberosum)detected by inter-simple sequence repeats(ISSRs)", Genome, 45, pp.481~484 (2002)
- Cantino, P. D., "Genera of the Labiatae, status and classification, In Advances in Labiate Science", pp.511~522 (1992), Royal Botanic Gardens
- Capo-chichi, L. J. A., "The use of molecular markers to study genetic diversity in Mucuna. Trop. Subtrop", Agroecosyst, 1, pp.309~318 (2003)
- Chen, J. M., "The extent of clonality and genetic diversity in the rare Caldesia grandis(Alismataceae), comparative results for RAPD and ISSR markers", Aquat. Bot, 84, pp.301~307 (2006)
- Dellaporta, S., "A plant DNA minipreparation : version l", Plant Mol. Biol. Rep, 1, pp.19~21 (1983)
- Dwivedi, S. L., "Assessment of genetic diversity among selected groundnut germplasm", Plant Breed, 120, pp.345~349 (2001)
- Fracaro, F., "Genetic variability in Hesperozygis ringens Benth. (Lamiaceae), an endangered aromatic and medicinal plant of Southern Brazil", Biochem. Genet, 44, pp.479~490 (2006)
- Gajera, B. B., "Assessment of genetic diversity in castor(Ricinus communis L. )using RAPD and ISSR markers. Ind", Crops Prod, 32, pp.491~498 (2010)
- Goulao, L., "Comparison between phenetic characterisation using RAPD and ISSR markers and phenotypic data of cultivated chestnut(Castanea sativa Mill.)", Genet. Resour. Crop Evol, 48, pp.329~338 (2001)
- Harley, R. M., "The families and genera of vascular plants, Vol. VII", pp.167~275 (2004), Springer- Verlag
- Hu, Y. P., "Genetic diversity of wild populations of Rheum tanguticum endemic to China as revealed by ISSR analysis", Biochem. Syst. Ecol, 38, pp.264~274 (2010)
- Ikbal, K. S. Boora., "Evaluation of genetic diversity in Jatropha curcus using RAPD markers", Indian. J. Biotechnol, 9, pp.50~57 (2010)
- Jung, B. S., "Encyclopedia of Korean Crude Drugs", pp.840~ (1990), Youngrim Sa
- Li, J. M., "Genetic structure of endangered Emmenopterys henryi Oliv. based on ISSR polymorphism and implications for its conservation", Genetica, 133, pp.227~234 (2008)
- Louis, L, "Biology of the Gene", pp.151~ (1980), C.V. Mosby Company
- Nagaoka, T., "Applicability of intersimple sequence repeat polymorphisms in wheat for use as DNA markers in comparison to RFLP and RAPD markers", Theor. Appl. Genet., 94, pp.597~602 (1997)
- Padmesh, P., "Estimation of genetic diversity in varieties of Mucuna pruriens using RAPD", Biol. Plant, 50, pp.367~372 (2006)
- Padmesh, P. P., "ISSR analysis reveals high intraspecific variation in Rauvolfia serpentina L. – a high value medicinal plant", Biochem. Syst. Ecol, 40, pp.192~197 (2012)
- Peng, S., "Genetic variation of Carthamus tinctorius L. and related species revealed by SRAP analysis", Biochem. Syst. Ecol, 36, pp.531~538 (2008)
- Qian, W., "Genetic variation within and among populations of a wild rice Oryza granulata from China detected by RAPD and ISSR markers", Theor. Appl. Genet., 102, pp.440~449 (2001)
- Ratnaparkhe, M. B., "Inter-simple-sequence-repeat (ISSR) polymorphisms are useful for finding markers associated with disease resistance gene clusters", Theor. Appl. Genet., 97, pp.515~519 (1998)
- Sofia, S. H., "Population genetic structure of Astyanax scabripinis(Teleostei, Characidae)from an urban stream", Hydrobiology, 553, pp.245~254 (2006)
- Thorne, R. F, "Classification and geography of the flowering plants", Bot. Rev., 58, pp.225~348 (1992)
- Wang, C., "Genetic differentiation in endangered Gynostemma pentaphyllum(Thunb. )Makino based on ISSR polymorphism and its implications for conservation", Biochem. Syst. Ecol, 36, pp.699~705 (2008)
- Wang, Z., "Survey of plant short tandem DNA repeats", Theor. Appl. Genet., 88, pp.1~6 (1994)
- Waugh, R., "Using RAPD markers for crop improvement", Trends Biotech, 10, pp.186~191 (1992)
- Weverstahl, P., "Volatile constituents of Agastache rugosa", J. Essent. Oil Res, 4, pp.585~587 (1992)
- Wolfe, A. D., "Assessing hybridization in natural populations of Penstemon(Scrophulariaceae)using hypervariable inter simple sequence repeat(ISSR)bands", Mol. Ecol, 7, pp.1107~1125 (1998)
- Wu, Y. G., "Genetic diversity analysis among and within populations of Pogostemon cablin from China with ISSR and SRAP markers", Biochem. Syst. Ecol, 38, pp.63~72 (2010)
- Yang, S., "Genetic diversity and population structure in Meconopsis quintuplinervia(Papaveraceae)", African J. Biotechnol, 9, pp.3048~3053 (2010)
- Yang, W. P., "Comparison of DNA marker technologies in characterizing plant genome diversity : variability in Chinese sorghums", Crop Sci, 36, pp.1669~1676 (1996)
- Ye, C. J., "RISSR as a new tool for genomic fingerprinting, mapping, and gene tagging", Plant Mol. Biol. Rep, 23, pp.167~177 (2005)
- Yee, E., "Diversity among selected Vigna angularis(Azuki)accessions on the basis of RAPD and AFLP markers", Crop Sci, 39, pp.268~275 (1999)
- POPGENE version 1-32. Microsoft Window.based freeware for population genetic gnalysis, "Yeh, F.", Molecular Biology and Biotechnology Center, University of Alberta, ~ (1999)
- Zietkiewicz, E., "Genome fingerprinting by simple sequence repeat(SSR). anchored polymerase chain reaction amplification", Genomics, 20, pp.176~183 (1994)
