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Genome-Wide SSR 마커를 이용한 주요 산지별 참당귀의 유전다양성 분석

Genetic Diversity of Angelica gigas Nakai Collected in Korea using Genome-Wide SSR Markers

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초록

Background: Angelica gigas Nakai has been used as an herbal medicine in Eastern Asia for treating disorders in women for a long time. To date there are no studies on the genetic diversity of A. gigas. The present study aimed to study the genetic diversity of A. gigas variants using genomewide simple sequence repeat (SSR) markers. Methods and Results: The genetic diversity of 199 variants of A. gigas cultivated in of different regions, was analyzed using 5 genome-wide SSR markers. The results revealed that the genetic variants were very diverse, and genetic analysis using the 5 SSR markers revealed high diversity among the variants. Conclusions: It is expected that the development of the true Angleical cultivar, by studying the system and group selection, can be achieved by genetic analysis using the developed markers, for generating a genetically fixed lineage and group selection.

Abstract

Background: Angelica gigas Nakai has been used as an herbal medicine in Eastern Asia for treating disorders in women for a long time. To date there are no studies on the genetic diversity of A. gigas. The present study aimed to study the genetic diversity of A. gigas variants using genomewide simple sequence repeat (SSR) markers. Methods and Results: The genetic diversity of 199 variants of A. gigas cultivated in of different regions, was analyzed using 5 genome-wide SSR markers. The results revealed that the genetic variants were very diverse, and genetic analysis using the 5 SSR markers revealed high diversity among the variants. Conclusions: It is expected that the development of the true Angleical cultivar, by studying the system and group selection, can be achieved by genetic analysis using the developed markers, for generating a genetically fixed lineage and group selection.

참고문헌 (30)

  1. 방경환, "SSR 마커를 이용한 국내산 인삼 품종 및 국외 수집종의 유전적 다양성 분석", 한국약용작물학회지, 19(5), pp.347~353 (2011), 한국약용작물학회
  2. Cavagnaro PF, "Genome-wide characterization of simple sequence repeats in cucumber (Cucumis sativus L.)", BMC Genomics, 11, pp.569~586 (2010)
  3. Cho TD, "Encyclopedia of Korean Herbs", pp.59~ (2006), Daewonsa
  4. 정종욱, "SSR 마커를 이용한 한국산과 중국산 구기자의 품종 판별", 한국약용작물학회지, 17(6), pp.445~451 (2009), 한국약용작물학회
  5. 길진수, "Angelica 속 식물의 종판별을 위한 연구현황 및 전망", Journal of Plant Biotechnology, 43(2), pp.151~156 (2016), 한국식물생명공학회[DOI]
  6. Gil JS, "Development of genome-wide SSR markers from Angelica gigas Nakai using next generation sequencing", Genes, 8, pp.238~ (2017)
  7. 조익현, "미토콘드리아 DNA 염기서열 변이를 이용한 인삼 종 판별 연구", 한국약용작물학회지, 21(2), pp.91~96 (2013), 한국약용작물학회[DOI]
  8. Kim HS, "Effective components and nitrite scavenging ability of root and leaves a Angelica gigas Nakai", The Korean Journal of Food and Cookery Science, 22, pp.957~965 (2006)
  9. 김정훈, "한국산 둥굴레속 식물의 형태적 특성 및 엽록체 DNA 염기서열을 이용한 유연관계 분석", 한국약용작물학회지, 22(6), pp.489~496 (2014), 한국약용작물학회[DOI]
  10. Kim JY, "Angelan isolated from Angelica gigas Nakai induces dendritic cell maturation through toll-like receptor 4", International Immunopharmacol, 7, pp.78~87 (2007)
  11. 김세림, "Simple sequence repeat marker development from Codonopsis lanceolata and genetic relation analysis", Journal of Plant Biotechnology, 43(2), pp.181~188 (2016), 한국식물생명공학회[DOI]
  12. Kim WJ, "Evaluation of genetic diversity of Polygonatum spp. by the analysis of simple sequence repeat(SSR)", Korean Herbal Medicine Informatics, 2, pp.41~47 (2014)
  13. Kofler R, "SciRoKo: A new tool for whole genome microsatellite search and investigation", Bioinfomatics, 23, pp.1683~1685 (2007)
  14. Lee MY, "Discrimination of Aralia continentalis root by the random amplified polymorphic DNA analysis and morphological characteristics", Korean Journal of Oriental Medicine, 7, pp.145~152 (2001)
  15. Li Gang, "Genetic Diversity Analysis of Mungbean Accessions from East and Central Asia Using SSR Markers", 한국국제농업개발학회지, 23(2), pp.185~193 (2011), 한국국제농업개발학회
  16. Liao C, "New insights into the phylogeny of Angelica and its allies(Apiaceae) with emphasis on east Asian species, inferred from nrDNA, cpDNA, and morphological evidence", Systematic Botany, 38, pp.266~281 (2013)
  17. Lu Y, "Isolation and characterization of 18polymorphic microsatellite markers for the “Female Ginseng”Angelica sinensis(Apiaceae) and cross-species amplification", Biochemical Systematics and Ecology, 61, pp.488~492 (2015)
  18. Mei Z, "Efficiency of improved RAPD and ISSR markers in assessing genetic diversity and relationships in Angelica sinensis(Oliv.) Diels varieties of China", Electronic Journal of Biotechnology, 18, pp.96~102 (2015)
  19. 문병철, "AFLP 분석을 통한 Curcuma속 수집종간 유전적 다양성 분석", 한국약용작물학회지, 21(6), pp.455~460 (2013), 한국약용작물학회[DOI]
  20. 박규림, "산지별 홍국발효 참당귀의 이화학적 특성 및 생리활성", 생명과학회지, 27(8), pp.919~929 (2017), 한국생명과학회
  21. 박상익, "당귀 종판별을 위한 엽록체 기반 SSR 마커 개발", 한국약용작물학회지, 24(4), pp.317~322 (2016), 한국약용작물학회[DOI]
  22. Ryu KS, "Studies on the coumarin constituents of the root of Angelica gigas Nakai. Isolation of decursinol angelate and assay of decursinol angelate and decursin", Korean Journal of Pharmacognosy, 21, pp.64~68 (1990)
  23. Seong NS, "Environmental variation of decursin content in Angelica gigas", Korean Journal of Crop Science, 38, pp.60~65 (1993)
  24. Shendure J, "Next-generation DNA sequencing", Nature Biotechnology, 26, pp.1135~1145 (2008)
  25. Song JY, "Development and characterization of 22 polymorphic microsatellite markers for the balloon flower Platycodon grandiflorum(Campanulaceae)", Genetics and Molecular Research, 11, pp.3263~3266 (2012)
  26. 엄유리, "Identification of Korean Ginseng (Panax ginseng) Cultivars Using Simple Sequence Repeat Markers", Plant Breeding and Biotechnology, 4(1), pp.71~78 (2016), 한국육종학회[DOI]
  27. 엄유리, "Genetic diversity analysis of Glycyrrhiza uralensis using 8 novel polymorphic microsatellite markers", Journal of Plant Biotechnology, 43(2), pp.174~180 (2016), 한국식물생명공학회[DOI]
  28. Williams JGK, "DNA polymorphisms amplified by arbitrary primers are useful as genetic markers", Nucleic Acids Research, 18, pp.6531~6535 (1990)
  29. Zalapa JE, "Using nextgeneration sequencing approaches to isolate simple sequence repeat(SSR) loci in the plant", American Journal of Botany, 99, pp.193~208 (2012)
  30. Zhu H, "Mining and validation of pyrosequenced simple sequence repeats (SSRs) from American cranberry(Vaccinium macrocarpon Ait.)", Theoretical and Applied Genetics, 124, pp.87~96 (2011)

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