Genetic variation and relationships of Artemisia capillaris Thunb. (Compositae) by RAPD Analysis
1
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Randomly Amplified Polymorphic DNA (RAPD) was performed to define the genetic variation and relationships of Artemisia capillaris. Fifteen populations by the distributions and habitat were collected to conduct RAPD analysis. RAPD markers were observed mainly between 300bp and 1600bp. Total 72 scorable markers from 7 primers were applied to generate the genetic matrix, and 69 bands were polymorphic and only 3 bands were monomorphic. The genetic dissimilarity matrix by Nei's genetic distance (1972) and UPGMA phenogram were produced from the data matrix. Populations of Artemisia capillaris were clustered with high genetic affinities and cluster patterns were correlated with distributional patterns. Two big groups were clustered as southern area group and middle area group. The closest OTUs were GW2 and GG1 in middle area group, and GB1 from southern area group was clustered with OTUs in middle area group. RAPD data was useful to define the genetic variations and relationships of A. capillaris.
Abstract
Randomly Amplified Polymorphic DNA (RAPD) was performed to define the genetic variation and relationships of Artemisia capillaris. Fifteen populations by the distributions and habitat were collected to conduct RAPD analysis. RAPD markers were observed mainly between 300bp and 1600bp. Total 72 scorable markers from 7 primers were applied to generate the genetic matrix, and 69 bands were polymorphic and only 3 bands were monomorphic. The genetic dissimilarity matrix by Nei's genetic distance (1972) and UPGMA phenogram were produced from the data matrix. Populations of Artemisia capillaris were clustered with high genetic affinities and cluster patterns were correlated with distributional patterns. Two big groups were clustered as southern area group and middle area group. The closest OTUs were GW2 and GG1 in middle area group, and GB1 from southern area group was clustered with OTUs in middle area group. RAPD data was useful to define the genetic variations and relationships of A. capillaris.
참고문헌 (24)
- Ahn, B.M, "What is In-Jin-Sook? Artemisia capillaris, Artemisia iwayomogi, and Artemisia annua", The Korean Journal of Hepatology., 16(4), pp.548~551 (2000)
- Cho, Y.H, "Research papers : Essential Oil Composition and Antibacterial Activity of Artemisia capillaries, Artemisia argyi, and Artemisia princeps", Kor. J. Intl. Agri., 13(4), pp.313~320 (2001)
- 최소라, "사철쑥의 수확시기에 따른 생육특성과 주성분 변화", 한국약용작물학회지, 15(3), pp.189~193 (2007), 한국약용작물학회
- 최소라, "사철쑥의 재배년수에 따른 생육과 약리성분 변화", 한국약용작물학회지, 16(1), pp.57~61 (2008), 한국약용작물학회
- 최소라, "사철쑥의 항산화성과 항균성", 한국약용작물학회지, 16(2), pp.112~117 (2008), 한국약용작물학회
- Doyle, J.J, "A rapid DNA isolation methods for small quantities of fresh tissues", Phytochemical Bulletin, 17, pp.144~163 (1987)
- Jung, T.H, "Korean Flora. vol. II" (1956), Shinjisa
- Kil, B.S, "Allelopathic Effects of Artemisia capillaris on the Selected Species", Korean J. Ecol., 22(1), pp.59~63 (1999)
- Kim, J.K, "Illustrated natural drugs encyclopedia.", pp.64~79 (1992), Namsandang
- 이미경, "사철쑥 추출물의 면역세포의 생육증진 및 세포독성", 한국약용작물학회지, 12(1), pp.36~42 (2004), 한국약용작물학회
- Lee, S.B, "Hepatoproteinactive effect of polysaccaride fraction from Artemisia iwayomogi in rat", 박사, pp.1996
- Lee, T.B, "Illustrated Flora of Korea", pp.755~ (1980), Hyangmunsa
- 임주락, "사철쑥의 발아 및 파종기별 생육특성", 한국약용작물학회지, 12(4), pp.295~299 (2004), 한국약용작물학회
- Lim, S.N, "Studies on the biological activities of Mugwort (Artemisia asiatica Nakai) Leaves", 석사, pp.1992
- Mata, R., "Sesquiterpene lactones of Artemisia klotzchiana", Phytochemistry, 24, pp.1515~1519 (1985)
- Nei, M, "Genetic distance between populations", Amer. Natur., 106, pp.283~292 (1972)
- Ohwi, J, "Flora of Japan. 2nd printing", Smithsonian Inst., ~ (1984)
- 조용구, "쑥 수집종의 형태적 형질과 RAPD분석에 의한 분류", 한국약용작물학회지, 13(6), pp.278~286 (2005), 한국약용작물학회
- Rho, T.H, "Growth Characteristics and Chemical Components in Local Collections of Artemisia sp", Kor. J. Medicinal Crop Sci., 1(2), pp.171~177 (1993)
- 서경순, "인진(茵蔯)으로 쓰이는 사철쑥과 더위지기 추출물의 항산화 활성", 한국자원식물학회지, 21(4), pp.292~298 (2008), 한국자원식물학회
- Song, K.W, "Phytochemical study of polysaccaride fraction from Artemisia species", 석사, pp.1996
- Williams, J., "DNA polymorphisms amplified by arbitary primers are useful as genetic markers", Nucleic Acids Research, 18, pp.6231~6239 (1990)
- Wu, T.S., "New constituents and antiplatelet aggregation and anti-HIV principles of Artemisia capillaris", Bioorganic & Medicinal Chemistry, 9, pp.77~83 (2001)
- Yook, C.S, "Medicinal Plants", Jinmyung Publishing Co., 293~298 (1977)