Effect of Carbohydrates on in vitro Shoot Growth of Various Prunus Species
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Carbohydrate sources are one of important factors associated with macro- and micro nutrients and phytohormones in vitro culture medium for shoot growth. The optimal carbohydrates for eight species of the genus Prunus which are economically important fruit crop was evaluated at the initiation and elongation stages. All carbohydrate seemed utilized for the bud break and leaf growth at the early stage of culture. However, shoot elongation and fresh weight of species tested were superior in the medium containing 90 mM of fructose or glucose rather than sucrose. There was no difference between glucose and fructose. Adventitious shoots from the axillary buds were induced in most species but no significant differences were observed except for two species (P. salicina ‘Shiro’ and P. tomentosa). These result demonstrated that glucose and fructose were suitable carbohydrate sources for diverse Prunus species than sucrose, although the response to the carbohydrates in the medium were slightly different in the species.
Abstract
Carbohydrate sources are one of important factors associated with macro- and micro nutrients and phytohormones in vitro culture medium for shoot growth. The optimal carbohydrates for eight species of the genus Prunus which are economically important fruit crop was evaluated at the initiation and elongation stages. All carbohydrate seemed utilized for the bud break and leaf growth at the early stage of culture. However, shoot elongation and fresh weight of species tested were superior in the medium containing 90 mM of fructose or glucose rather than sucrose. There was no difference between glucose and fructose. Adventitious shoots from the axillary buds were induced in most species but no significant differences were observed except for two species (P. salicina ‘Shiro’ and P. tomentosa). These result demonstrated that glucose and fructose were suitable carbohydrate sources for diverse Prunus species than sucrose, although the response to the carbohydrates in the medium were slightly different in the species.
참고문헌 (20)
- Ahmad, T, "Comparison of sucrose and sorbitol as main carbon energy sources in micropropagation of peach rootstock GF-677", Pak. J. of Bot, 39(4), pp.1269~1275 (2007)
- Bahmani, R, "Influence of carbon sources and their concentrations on rooting and hyperhydricity of apple rootstock MM.106", World Appl Sci J, 6(11), pp.1513~1517 (2009)
- Channuntapipat, C, "Micropropagation of almond cultivars Nonpareil and Ne Plus Ultra and the hybrid rootstock Titan x Nemaguard", Sci Hort, 98, pp.473~484 (2003)
- Cheong, E.J, "A study on mass propagation of Prunus yedoensis Matsumura from Jeju using in vitro culture techniques", Ph.D, pp.2000
- De Neto, V.B.P, "Carbon sources and their osmotic potential in plant tissue culture: does it matter", Sci Hort, 97, pp.193~202 (2003)
- Harada, H, "Micropropagation of Prunus mume", Plant Cell Tiss. Org. Cult, 46, pp.265~267 (1996)
- Howell, W.E, "Heat treatment, chemo-therapy and hydroponic culture for obtaining virus-free trees of sweet cherry", Acta Hort, 550, pp.455~457 (2001)
- Kalinina, A, "Micropropagation of ornamental Prunus spp. and GF305 peach, a Prunus viral indicator", Plant Cell Rep, 26, pp.927~935 (2007)
- Kromer, K, "Analysis of soluble carbohydrates, proteins and lipids in shoots of M-7 apple rootstock cultured in vitro during regeneration of adventitious roots", J Plant Physiol, 156, pp.775~782 (2000)
- Kozai, T, "Biotechnology in Agriculture and Forestry. Vol 17. High-Tech and Micropropagation I", pp.313~343 (1991), Springer
- 이재웅, "‘자랑’ 포도 생장점 배양으로부터 무병주 식물 대량번식에 미치는 배지 구성물질과 생장 조절제의 영향", 한국자원식물학회지, 26(2), pp.303~309 (2013), 한국자원식물학회[DOI]
- Li, M.S, "Starch accumulation is associated with adventitious root formation in hypocotyls cuttings of Pinus radiata", J Plant Growth Regul, 19, pp.423~428 (2000)
- Lloyd, G, "Commercially feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot tip culture", Proc Int Plant Prop Soc, 30, pp.421~427 (1981)
- Navarro, L, "Shoot tip grafting in vitro for elimination of viruses in peach plants (Prunus persica Batsch)", Acta Hort, 130, pp.185~192 (1982)
- Stein, A, "Responses of micropropagated peach cultivars to thermotherapy for the elimination of Prunus necrotic ringspot virus", Assoc. Appl. Bio, 119, pp.265~271 (1991)
- Rizqi, A, "Recovery of virusfree almond plants by improved in vitro shoot-tip grafting", Acta Hort, 550, pp.445~447 (2001)
- Waterworth, H, "Processing foreign plant germplasm at the National Plant Germplasm Quarantine Center", Plant Dis, 77(9), pp.854~860 (1993)
- Yaseen, M, "Assessment of apple rootstocks M9 and M26 for in vitro rooting potential using different carbon sources", Pak J Bot, 41(2), pp.769~781 (2009)
- Yaseen, M, "In vitro shoot proliferation competence of apple rootstocks M.9 and M.26 on different carbon sources", Pak J Bot, 41(4), pp.1781~1795 (2009)
- Yaseen, M, "Review: role of carbon sources for in vitro plant growth and development", Mol Bio Rep, 40, pp.2837~2849 (2013)