Antioxidant Activity of Several Cabbage (Brassica oleracea L.) Cultivars
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Total phenol, flavonoid and antioxidant components of cabbage leaf samples derived from different cultivar were determined. Total phenol compound content showed the highest amount in methanol extracts from ‘YR Howol’ cultivar (11.72 ㎎/g), followed by ‘Harutame’ (10.66㎎/g), ‘Winstar’ (10.34 ㎎/g) and YR Hero (10.20 ㎎/g). The highest amount of total flavonoid content was observed from the methanol extracts of Harutame (5.39 ㎎/g), followed by Winstar (4.28 ㎎/g), Wialhowol (4.10 ㎎/g). The SOD enzyme activity showed a high activity of ‘YR Hogeo’ cultivar, and the cultivar of ‘YR Howol’ cultivar showed the lowest activity of SOD. The activity of CAT and APX showed higher values ‘Ogane’ and ‘YR Hogeol’ cultivars than the other cultivars. The POD activities showed relatively high values ‘Ogane’ and ‘YR Howol’ cultivars compared with other cultivars. The free radical (DPPH) scavenging activity showed lower IC50 values of ‘Harutame’ (15.71) and ‘YR Howol’ cultivar (16.88), however methanol extract of ‘YR Hero’ cultivar (22.49) being the highest. The extracts of all cabbage cultivars in the reaction solution of pH 1.2 could be decomposed nitrite more than 50%. Especially, the cultivar ‘YR Hogeol’ and ‘Ogane’ showed a relatively high nitrite scavenging activity for each 60.13% and 57.20% respectively. The IC50 values of antioxidant activity determined by ABTS were lower in ‘Harutame’ (17.04) and ‘YR Howol’ cultivar (17.97), and its results observed similar with values obtained from the same extracts by DPPH method. The result of this study suggests that the methanol extract of Brassica oleracea L. contains the high amount of phenolic and higher radical scavenging activities.
Abstract
Total phenol, flavonoid and antioxidant components of cabbage leaf samples derived from different cultivar were determined. Total phenol compound content showed the highest amount in methanol extracts from ‘YR Howol’ cultivar (11.72 ㎎/g), followed by ‘Harutame’ (10.66㎎/g), ‘Winstar’ (10.34 ㎎/g) and YR Hero (10.20 ㎎/g). The highest amount of total flavonoid content was observed from the methanol extracts of Harutame (5.39 ㎎/g), followed by Winstar (4.28 ㎎/g), Wialhowol (4.10 ㎎/g). The SOD enzyme activity showed a high activity of ‘YR Hogeo’ cultivar, and the cultivar of ‘YR Howol’ cultivar showed the lowest activity of SOD. The activity of CAT and APX showed higher values ‘Ogane’ and ‘YR Hogeol’ cultivars than the other cultivars. The POD activities showed relatively high values ‘Ogane’ and ‘YR Howol’ cultivars compared with other cultivars. The free radical (DPPH) scavenging activity showed lower IC50 values of ‘Harutame’ (15.71) and ‘YR Howol’ cultivar (16.88), however methanol extract of ‘YR Hero’ cultivar (22.49) being the highest. The extracts of all cabbage cultivars in the reaction solution of pH 1.2 could be decomposed nitrite more than 50%. Especially, the cultivar ‘YR Hogeol’ and ‘Ogane’ showed a relatively high nitrite scavenging activity for each 60.13% and 57.20% respectively. The IC50 values of antioxidant activity determined by ABTS were lower in ‘Harutame’ (17.04) and ‘YR Howol’ cultivar (17.97), and its results observed similar with values obtained from the same extracts by DPPH method. The result of this study suggests that the methanol extract of Brassica oleracea L. contains the high amount of phenolic and higher radical scavenging activities.
참고문헌 (31)
- Ahmed, M.F, "Phytochemical studies and antioxidant activities of Brassica Oleracea L. var. Capitata", Int. J. Pharm. Sci, 4(3), pp.374~378 (2012)
- Apel, K, "Reactive oxygen species: Metabolism, oxidative stress, and signal transduction", Annu. Rev. Plant Biol, 5, pp.373~399 (2004)
- Asada, K, "Causes of Photooxidative Stress and Amelioration of Defense Systems in Plants", pp.77~105 (1994), CRC Press
- Asish, K., "Salt tolerance and salinity effects on plants", A review Ecotoxicol. Environ. Saf, 60, pp.324~349 (2005)
- Bonnet, M, "Effects of zinc and influence of Acremonium lolii on growth parameters, chlorophyll a fluorescence and antioxidant enzyme activities of ryegrass (Lolium perenne L. cv Apollo)", J. Exp. Bot, 51(346), pp.945~953 (2000)
- 부희옥, "Assessment on Antioxidant Potential and Enzyme Activity of Some Economic Resource Plants", 한국자원식물학회지, 25(3), pp.349~356 (2012), 한국자원식물학회[DOI]
- DuBok Choi, "Effect of bamboo oil on antioxidative activity and nitrite scavenging activity", Journal of Industrial and Engineering Chemistry, 14(6), pp.765~770 (2008), 한국공업화학회
- Chun, O.K, "Antioxidant properties of raw and processed cabbage", Int. J. Food Sci. Nutr, 55(3), pp.191~199 (2004)
- Dionisio-Sese, M.L, "Antioxidant responses of rice seedlings to salinity stress", Plant Sci, 135, pp.1~9 (1998)
- Ferreres, F, "Chemical composition and antioxidant activity of tronchuda cabbage internal leaves", Eur. Food Res. Technol, 222, pp.88~98 (2006)
- Foyer, C.H, "Protection against oxygen radicals: an important defence mechanism studied in transgenic plants", Plant Cell Env, 17, pp.507~523 (1994)
- Galati, G, "Potential toxicity of flavonoids and other dietary phenolics: significance for their chemopreventive and anticancer properties", Free Radic. Biol. Med, 37(3), pp.287~303 (2004)
- Gaspar, T, "A two step control of basic and acid peroxidases and its significance for growth and development", Plant Physiol, 64, pp.418~423 (1985)
- Hollman, P.C, "Role of dietary flavonoids in protection against cancer and coronary heart disease", Biochem. Soc. Trans, 24(3), pp.785~789 (1996)
- 임무현, "솔잎발효추출물의 효소적 저해활성 및 아질산염 소거작용", 한국식품저장유통학회지, 11(1), pp.94~99 (2004), 한국식품저장유통학회
- Huang, Y, "Scavenging effect of total flavonoids of Lycium barbarum L. on active oxygen radicals and inhibitory effects on heat output from L 1210 cells", Wei Sheng Yen Chiu, 27, pp.109~111 (1998)
- Ismail, A, "Total antioxidant activity and phenolic content in selected vegetables", Food Chem, 87, pp.581~586 (2004)
- Kang, H.M, "Chilling tolerance of maize, cucumber and rice seedling leaves and roots are differentially affected by salicylic acid", Physiol. Plant, 115, pp.571~576 (2002)
- Kato, F.T, "Mutagenesis by carcinogenic nitroso compounds", J. Cell Physiol, 78, pp.139~144 (1971)
- Kusznierewicz, B, "Partial characterization of white cabbage (Brassica oleracea var. capitata f. alba) from different regions by glucosinolates, bioactive compounds, total antioxidant activities and proteins", LWT, 41, pp.1~9 (2008)
- Lee, S.C, "Effect of far-infrared irradiation on catechins and nitrite scavenging activity of green tea", J. Agric. Food Chem, 54, pp.399~403 (2006)
- Mittler, R, "Oxidative stress, antioxidants and stress tolerance", Trends Plant Sci, 7, pp.405~410 (2002)
- Najami, N, "Ascorbate peroxidase gene family in tomato: its identification and characterization", Mol. Genet. Genom, 279, pp.171~182 (2008)
- Shahidi, F, "Phenolic Compounds in Foods and Natural Health Products", pp.1~8 (2005), ACS
- 성낙주, "유자의 화학적 특성 및 아질산염 소거작용", 한국식품영양과학회지, 34(4), pp.496~502 (2005), 한국식품영양과학회
- Stratil, P, "Determination of total content of phenolic compounds and their antioxidant activity in vegetables―evaluation of spectrophotometric methods", J. Agri. Food Chem, 54, pp.607~616 (2006)
- Wheeler, G.L, "The biosynthetic pathway of vitamin C in higher plants", Nature, 393, pp.363~369 (1998)
- Wojtaszek, P, "Oxidative burst: an early plant response to pathogen infection", Biochem. J, 322, pp.681~692 (1997)
- Yokozawa, T, "Study on the inhibitory effect of tannins and flavonoids against the 1,1-diphenyl-2-picrylhydrazyl radical", Biochemical Phamacology, 56, pp.213~222 (1998)
- Zhou, B.Y, "Effects of abscisic acid on antioxidant systems of Stylosanthes guianensis (Aublet) Sw. under chilling stress", Crop Sci, 45, pp.599~605 (2005)
- Zhu, N, "Identification of reaction products of (-)-epigallocatechin, (-)-epigallocatechin gallate and pyrogallol with 2,2-diphenyl-1- picrylhydrazyl radical", Food Chemistry, 73, pp.345~349 (2001)