Efficient in vitro Clonal Propagation of Fast Growing Salix gracilistyla
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This study was determined optimal condition as culture medium, pH, carbohydrate source andgrowth regulators through axillary buds cultures of Salix gracilistyla. S. gracilistyla shoots wereobtained from axillary bud on 1/2MS basal medium after 2 weeks of culture. Highest shootgrowth was showed in the MS medium(GI= 2.43). Shoot growth was highest in 9% sucrosecompared to other concentration. BAP affects axillary shoot multiplication in of S. gracilistyla. Among nutrients, Ca2+ was not affected shoot number of S. gracilistyla. However, NH4+, NO3-,and K+ is greatly influenced shoot number. However, Ca2+ was affected in shoot length of S. gracilistyla. Feeding of Mg2+ was not affected in shoot length. Root number is greatlyinfluenced by NH4+, NO3-, and K+, however NH4+ and K+ was not affected root number of S. gracilistyla. NH4+ was affected root length, however Mg4+ was not affected root length. Treatment of 0.1mg/L IBA promoted in vitro rooting. In vitro rooted plantlets were successfullytransferred to pots for 4weeks hardening process. Later, these plants were transferred to soilwith above 90% survival rate in BS+SD(2:1). These results could be easily adopted forcommercial large scale cultivation.
Abstract
This study was determined optimal condition as culture medium, pH, carbohydrate source andgrowth regulators through axillary buds cultures of Salix gracilistyla. S. gracilistyla shoots wereobtained from axillary bud on 1/2MS basal medium after 2 weeks of culture. Highest shootgrowth was showed in the MS medium(GI= 2.43). Shoot growth was highest in 9% sucrosecompared to other concentration. BAP affects axillary shoot multiplication in of S. gracilistyla. Among nutrients, Ca2+ was not affected shoot number of S. gracilistyla. However, NH4+, NO3-,and K+ is greatly influenced shoot number. However, Ca2+ was affected in shoot length of S. gracilistyla. Feeding of Mg2+ was not affected in shoot length. Root number is greatlyinfluenced by NH4+, NO3-, and K+, however NH4+ and K+ was not affected root number of S. gracilistyla. NH4+ was affected root length, however Mg4+ was not affected root length. Treatment of 0.1mg/L IBA promoted in vitro rooting. In vitro rooted plantlets were successfullytransferred to pots for 4weeks hardening process. Later, these plants were transferred to soilwith above 90% survival rate in BS+SD(2:1). These results could be easily adopted forcommercial large scale cultivation.
참고문헌 (38)
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