잎 절편 배양을 이용한 배 ‘신고’ (Pyrus pyrifolia cv. Niitaka)의 신초 재분화
Shoot regeneration via culture of leaf explants in pear (Pyrus pyrifolia cv. Niitaka)
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초록
Genetic manipulation of pear (Pyrus pyrifolia Nakai) breeding is still difficult due to lack of reliable regeneration system. The aim of this research is to establish shoot regeneration system from leaf explants for pear (P. pyrifolia cv. Niitaka) using various concentrations of plant growth regulators and carbon source supplemented to medium. The highest regeneration rate of about 20% was found on a medium containing 4.4 g/L of Murashige and Skoog (MS) without vitamins, Linsmaier and Skoog (LS)vitamins were added separately. Leaf explants of pear were cultured on MS medium containing 7 g/L of Daishin agar supplemented with various concentrations of NAA (0.01,0.05, 0.1, 0.5 mg/L) in combination with BA(3, 5, 10 mg/L)for shoot regeneration. In medium with 5 mg/L of BA and 0.01 mg/L of NAA, adventitious shoot regeneration rate was higher than others treated. The optimal results were observed using MS medium supplemented with 30 g/L sorbitol as carbon source on regeneration system. Sorbitol is considered better carbon source than sucrose for shoot regeneration of pear (P. pyrifolia cv. Niitaka). In order to increase of shoot regeneration in pear (P. pyrifolia cv. Niitaka), plant agar and Daishin agar used as gelling agents, Daishin agar is more efficient in shoot regeneration.
Abstract
Genetic manipulation of pear (Pyrus pyrifolia Nakai) breeding is still difficult due to lack of reliable regeneration system. The aim of this research is to establish shoot regeneration system from leaf explants for pear (P. pyrifolia cv. Niitaka) using various concentrations of plant growth regulators and carbon source supplemented to medium. The highest regeneration rate of about 20% was found on a medium containing 4.4 g/L of Murashige and Skoog (MS) without vitamins, Linsmaier and Skoog (LS)vitamins were added separately. Leaf explants of pear were cultured on MS medium containing 7 g/L of Daishin agar supplemented with various concentrations of NAA (0.01,0.05, 0.1, 0.5 mg/L) in combination with BA(3, 5, 10 mg/L)for shoot regeneration. In medium with 5 mg/L of BA and 0.01 mg/L of NAA, adventitious shoot regeneration rate was higher than others treated. The optimal results were observed using MS medium supplemented with 30 g/L sorbitol as carbon source on regeneration system. Sorbitol is considered better carbon source than sucrose for shoot regeneration of pear (P. pyrifolia cv. Niitaka). In order to increase of shoot regeneration in pear (P. pyrifolia cv. Niitaka), plant agar and Daishin agar used as gelling agents, Daishin agar is more efficient in shoot regeneration.
참고문헌 (32)
- Banno K, "In vitro propagation of Japanese pear cultivars", J Japan Soc Hort Sci, 58, pp.37~42 (1982)
- Bustos MM, "Transient gene expression in electroporated bean cotyledon protoplasts", Plant Mol Biol Rep, 9, pp.322~332 (1991)
- Carboni E, "In vitro shoot regeneration from leaves of wild pear", Plant Cell Tissue Organ Cult, 59, pp.1~7 (1999)
- Challice JS, "Numerical taxonomic studies of the genus Pyrus using both chemical and botanical characters", Bot J Linn Soc, 67, pp.121~148 (1973)
- Cheng TY, "Micropropagation of clonal fruit tree rootstocks", Compact Rruit Trees, 12, pp.127~137 (1979)
- Chevreau E, "The effect of mother plant pretreatment and explants choice on regeneration from in vitro pear leaves", Acta Hort, 336, pp.263~268 (1993)
- Chevreau E, "Effect of gelling agents and antibiotics on adventitious bud regeneration from in vitro leaves of pear", In Vitro Cell Dev Biol Plant, 33, pp.173~179 (1997)
- Chevreau E, "Adventitious shoot regeneration from leaf tissue of three pear (Pyrus sp.) cultivars in vitro", Plant Cell Rep, 7, pp.688~691 (1989)
- 천재안, "황금배(Pyrus pyrifolia Nakai) 잎 조직으로부터 기내 신초 재분화", Journal of Plant Biotechnology, 39(4), pp.288~294 (2012), 한국식물생명공학회
- Garcia R, "Influence of type of explants, plant growth regulators, salt composition of basal medium, and light on callogenesis and regeneration in Passiflora suberosa L. (Passifloraceae)", Plant Cell Tissue Organ Cult, 106, pp.47~54 (2011)
- Hennayake CK, "An Efficient and reproducible in vitro plant regeneration from leaf discs in pear cultivars (Pyrus spp)", Plant Biotechnol, 20, pp.283~289 (2003)
- Huetteman CA, "Thidiazuron: A potent cytokine for woody plant tissue culture", Plant Cell Tissue Organ Cult, 33, pp.105~119 (1993)
- Kadota M, "Effect of cytokinin types and their concentrations on shoot proliferation and hyperhydricity in in vitro pear cultivar shoots", Plant Cell Tissue Organ Cult, 72, pp.261~265 (2003)
- Kapila J, "An Agrobacterium mediated transient expression system for intact leaves", Plant Sci, 122, pp.101~108 (1997)
- 김세희, "Agrobacterium 공동배양을 이용한 포도 재분화율 향상과 GUS 유전자의 발현", Journal of Plant Biotechnology, 38(4), pp.308~314 (2011), 한국식물생명공학회
- Lane WD, "Regeneration of pear plants from shoot meristemtips", Plant Sci Lett, 16, pp.337~342 (1979)
- Lee CH, "In vitro shoot tip culture of pear ‘Niitaka’ as related to tree vigor, sampling time and plant growth regulators", Korean J plant tissue culture, 25, pp.159~163 (1989)
- Chang-Hoo Lee, "배(Pyrus pyrifolia) '신고' 잎으로부터 효과적인 재분화 시스템 개발", Horticulture, Environment, and Biotechnology, 43(3), pp.271~274 (2002), 한국원예학회
- Lemos EEP Baker DA, "Shoot regeneration in response to carbon source on intermodal explants of Annona muricata L", Plant Growth Regul, 25, pp.105~112 (1998)
- Linsmaier EM, "Organic growth factor requirements of tobacco tissue cultures", Physiol Plant, 18, pp.100~127 (1965)
- LI ZT, "Optimizing Agrobacterium-mediated transformation of grapevine", In Vitro Cell Dev Biol-Plant, 42, pp.220~227 (2006)
- Moon JG, "Effect of growth regulators on plant regeneration from the cotyledon explants in oriential melon (Cucumis melo L.)", Kor J Plant Tiss Cult, 27, pp.1~6 (2000)
- Murashige T, "A revised medium for rapid growth and bioassay with tobacco tissue cultures", Phyiol Plant, 15, pp.473~497 (1962)
- Nayak PS, "Direct shoot regeneration from foliar explants of apiphytic orchid, Acampe praemorsa (Roxb.)", Blatter and McCann. Plant Cell Rep, 16, pp.583~586 (1997)
- Ochatt SJ, "Plant regeneration from mesophyll protoplasts of Williams’ Bon Chretien (syn. Bartlett) pear (Pyrus communis L.)", Plant Cell Rep, 7, pp.587~589 (1988)
- Rubtsov GA, "Geographical distribution of the genus Pyrus and trends and factors in its evolution", Am Nat, 78, pp.358~366 (1944)
- Seo MS, "Investigation of transformation efficiency of rice using Agrobacterium tumefaciens and high transformation of GPAT (glycerol-3-phosphate acyltransferase) gene relative to chilling tolerance", Korean J Plant Biotechnology, 29, pp.85~92 (2002)
- Singha S, "Influence of two commercial agars on in vitro shoot proliferation of ‘Almey’ crabapple and Pyrus communis ‘Seckel’", Hort Science, 19, pp.227~228 (1984)
- Sotiropoulos TE, "Sucrose and sorbitol effects on shoot growth and proliferation in vitro nutritional status and peroxidase and catalase isoenzymes of M9 and MM106 apple (Malus domestica Borkh.) rootstocks", Europ J Hort Sci, 71, pp.114~119 (2006)
- Tukey HB, "Artificial culture methods for isolated embryos of deciduous fruits", Proc Am Soc Hort Sci, 32, pp.630~665 (1934)
- Vidal JR, "Highefficiency biolistic co-transformation and regeneration of ‘Chardonnay’ (Vitis vinifera L.) containing npt-II and antimicrobial peptide genes", Plant Cell Rep, 22, pp.252~260 (2003)
- Yotsuya D, "Effects of GA4+7 and BA on the in vitro growth of ‘Kosui’ leaf bud", Sci Rep Fac Kobe Univ, 14, pp.45~49 (1980)