Murine Macrophage RAW 264.7 세포에서누은분홍잎(Acrosorium yendoi Yamada)의 추출물과 에틸아세테이트 분획물에 대한 항염증 효과
Anti-inflammatory Effects on 80% Ethanol Extract and Ethyl Acetate Fraction of Acrosorium yendoi Yamada in Murine Macrophage RAW 264.7 Cells
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본 연구는 누은분홍잎(Acrosorium yendoi Yamada) 80% 에탄올를 가지고 추출한 후 추출물을 극성에 따라 순차적으로 용매분획을 실시하여, 80% 에탄올 추출물 및 그 분획물들의 염증반응의 주체가 되는 대식세포 계열인 RAW 264.7 세포에서 LPS 로 유도된 NO의 생성억제 효과, 그리고 iNOS와 COX-2의 단백질 발현 억제효과, PGE2 생성 억제 효과 및 TNF-α와 IL-6등과같은 pro-inflammatory cytokines 생성 억제효과 등을 알아보았다. 대식세포 계열인 RAW 264.7 세포에 LPS로 자극을 주고누은분홍잎 80% 에탄올 추출물 및 분획물을 처리하여 확인해본결과, 에틸아세테이트 분획물에서 NO와 PGE2 생성 억제 활성
Abstract
This study describes a preliminary evaluation of the anti-inflammatory activity of Acrosorium yendoi Yamada extracts. A. yendoi Yamada was extracted using 80% ethanol and then fractionated sequentially with n-hexane, ethyl acetate and butanol. To screen for anti-inflammatory agents effectively, we first examined the inhibitory effect of 80% EtOH extract and solvent fractions of A. yendoi Yamada on the production of pro-inflammatory factors and cytokines stimulated with lipopolysaccharide. In addition, we examined the inhibitory effect of 80% EtOH extract and solvent fractions of A. yendoi Yamada on pro-inflammatory mediators (NO, iNOS, PGE2, and COX-2) in RAW 264.7 cells. In the sequential fractions of n-hexane and EtOAc inhibited the NO and PGE2 production and the protein level of iNOS and COX-2, and protein expression of pro-inflammatory cytokines (TNF-α, and IL-6). These results suggest that A. yendoi Yamada may have significant effects on inflammatory factors and may be provided as possible anti-inflammatory therapeutic seaweed.
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