LPS로 유도된 RAW 264.7 대식세포에 대한 애기외톨개 모자반(Myagropsis yendoi) 에틸아세테이트 분획물의 항염증 효과
Anti-inflammatory Effect of an Ethyl Acetate Fraction from Myagropsis yendoi on Lipopolysaccharides-Stimulated RAW 264.7 Cells
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초록
An ethanolic extract from Myagropsis yendoi was fractionated using several solvents. Among these, an ethyl acetatefraction (Myagropsis yendoi ethyl acetate fraction: MYE) showed the highest anti-inflammatory activity based oninhibition of lipopolysaccharides (LPS)-induced nitric oxide (NO) production in RAW 264.7 cells. We thus investigatedthe molecular mechanisms underlying MYE’s inhibitory effects. Pretreatment of cells with up to 30 μg/mL ofMYE significantly inhibited NO production and inducible nitric oxide synthase expression in a dose-dependent manner(P<0.05). Similarly, MYE markedly reduced the production of pro-inflammatory cytokines, such as interleukin(IL)-1β, IL-6, and tumor necrosis factor (TNF)-α, as well as their mRNA levels. While the nuclear translocation ofnuclear factor-kappa B (NF-κB) was strongly suppressed by MYE, the activation of a nuclear factor erythroid 2-relatedfactor (Nrf2) was increased. Moreover, MYE significantly reduced the phosphorylation of JNK, p38 MAPK,and phosphatidylinositol 3-kinase/Akt in LPS-stimulated cells. These results indicate that MYE contains anti-inflammatorycompounds, and that it might be used as a dietary supplement for the prevention of inflammatory diseases.
Abstract
An ethanolic extract from Myagropsis yendoi was fractionated using several solvents. Among these, an ethyl acetatefraction (Myagropsis yendoi ethyl acetate fraction: MYE) showed the highest anti-inflammatory activity based oninhibition of lipopolysaccharides (LPS)-induced nitric oxide (NO) production in RAW 264.7 cells. We thus investigatedthe molecular mechanisms underlying MYE’s inhibitory effects. Pretreatment of cells with up to 30 μg/mL ofMYE significantly inhibited NO production and inducible nitric oxide synthase expression in a dose-dependent manner(P<0.05). Similarly, MYE markedly reduced the production of pro-inflammatory cytokines, such as interleukin(IL)-1β, IL-6, and tumor necrosis factor (TNF)-α, as well as their mRNA levels. While the nuclear translocation ofnuclear factor-kappa B (NF-κB) was strongly suppressed by MYE, the activation of a nuclear factor erythroid 2-relatedfactor (Nrf2) was increased. Moreover, MYE significantly reduced the phosphorylation of JNK, p38 MAPK,and phosphatidylinositol 3-kinase/Akt in LPS-stimulated cells. These results indicate that MYE contains anti-inflammatorycompounds, and that it might be used as a dietary supplement for the prevention of inflammatory diseases.
참고문헌 (43)
- Awad NE, "Seasonal variation of the lipoidal matters and hypolipidaemic activity of the red alga Corallina officinalis L", Phytother Res, 17, pp.19~25 (2003)
- Balkwill F, "TNF-alpha in promotion and progression of cancer", Cancer Metastasis Reviews, 25, pp.409~416 (2006)
- Beutler B, "The biology of cachectin", TNF- -a primary mediator of the host response, 1989, pp.7, 625~655
- Chen XL, "Induction of cytoprotective genes through Nrf2", antioxidant response element pathway: a new therapeutic approach for the treatment of inflammatory diseases, 2004, pp.10, 879~891
- Chen Z, "Signal-induced site-specific phosphorylation targets I kappa B alpha to the ubiquitinproteasome pathway", Genes Dev, 9, pp.1586~1597 (1995)
- D'Acquisto F, "Involvement of NF-kappaB in the regulation of cyclooxygenase-2 protein expression in LPSstimulated J774 macrophages", FEBS Lett, 418, pp.175~178 (1997)
- "The characteristics and kinds of Sargassum" (2008)[URL]
- Dinarello CA, "Cytokines as endogenous pyrogens", J Infect Dis, 179(2), pp.S294~S304 (1999)
- González del Val A, "Screening of antimicrobial activities in red, green and brown macroalgae from Gran Canaria (Canary Islands, Spain)", Int Microbiol, 4, pp.35~40 (2001)
- Guha, M, "LPS induction of gene expression in human monocytes", Cell signal, 13, pp.85~94 (2001)
- Gwon WG, "Hexane Fraction from Sargassum fulvellum Inhibits Lipopolysaccharide-Induced Inducible Nitric Oxide Synthase Expression in RAW 264.7 Cells via NF-kappaB Pathways", Am J Chin Med, 41, pp.565~584 (2013)
- Ha YM, "Beta(1)-adrenergic receptor- mediated HO-1 induction, via PI3K and p38 MAPK, by isoproterenol in RAW 264.7 cells leads to inhibition of HMGB1 release in LPS-activated RAW 264.7 cells and increases in survival rate of CLP-induced septic mice", Biochem Pharmacol, 82, pp.769~777 (2011)
- Hattori Y, "Lipopolysaccharide activates Akt in vascular smooth muscle cells resulting in induction of inducible nitric oxide synthase through nuclear factor-kappa B activation", Eur J Pharmacol, 481, pp.153~158 (2003)
- Jang BC, "Catalase induced expression of inflammatory mediators via activation of NF-kappaB, PI3K", AKT, p70S6K, and JNKs in BV2 microglia, 2005, pp.17, 625~633
- Joung EJ, "Anti-inflammatory effects of phlorofucofuroeckol B-rich ethyl acetate fraction obtained from Myagropsis myagroides on lipopolysaccharide-stimulated RAW 264.7 cells and mouse edema", Int Immunopharmacol, 14, pp.471~480 (2012)
- Joung EJ, "Anti-inflammatory effect of ethanolic extract from Myagropsis myagroides on murine macrophages and mouse ear edema.", BMC Complement Altern Med, 12, pp.171~ (2012)
- Jung HA, "Antiinflammatory activity of edible brown alga Eisenia bicyclis and its constituents fucosterol and phlorotannins in LPSstimulated RAW264.7 macrophages", Food Chem Toxicol: an international journal published for the British Industrial Biological Research Association, 59, pp.199~206 (2013)
- 정현아, "Anti-adipogenic activity of the edible brown alga Ecklonia stolonifera and its constituent fucosterol in 3T3-L1 adipocytes", Archives of Pharmacal Research, 37(6), pp.713~720 (2014), 대한약학회
- Kang GJ, "Sargaquinoic acid isolated from Sargassum siliquastrum inhibits lipopolysaccharideinduced nitric oxide production in macrophages via modulation of nuclear factor-kappaB and c-Jun N-terminal kinase pathways", Immunopharmacol Immunotoxicol, 35, pp.80~87 (2013)
- Kim AR, "Phlorofucofuroeckol A suppresses expression of inducible nitric oxide synthase, cyclooxygenase-2, and pro-inflammatory cytokines via inhibition of nuclear factorkappaB, c-Jun NH2-terminal kinases, and Akt in microglial cells", Inflammation, 36, pp.259~271 (2013)
- Kim KC, "Up-regulation of Nrf2-mediated heme oxygenase-1 expression by eckol, a phlorotannin compound, through activation of Erk and PI3K", Akt, 2010, pp.42, 297~305
- Kim KN, "Fucoxanthin inhibits the inflammatory response by suppressing the activation of NF-kappaB and MAPKs in lipopolysaccharide-induced RAW 264.7 macrophages", Eur J Pharmacol, 649, pp.369~375 (2010)
- Kim S, "Anti-inflammatory effect of hexane fraction from Myagropsis myagroides ethanolic extract in lipopolysaccharide-stimulated BV-2 microglial cells", J Pharm Pharmacol, 65, pp.895~906 (2013)
- Kim S, "Anti-inflammatory effects of sargachromenolrich ethanolic extract of Myagropsis myagroides on lipopolysaccharide- stimulated BV-2 cells", BMC Complement Altern Med, 14, pp.231~ (2014)
- Lawrence T, "Anti-inflammatory lipid mediators and insights into the resolution of inflammation", Nat Rev Immunol, 2, pp.787~795 (2002)
- Lebovic DI, "Induction of an angiogenic phenotype in endometriotic stromal cell cultures by interleukin-1beta", Mol Hum Reprod, 6, pp.269~275 (2000)
- Lee JW, "3,4,5-trihydroxycinnamic acid inhibits lipopolysaccharide (LPS)-induced inflammation by Nrf2 activation in vitro and improves survival of mice in LPS-induced endotoxemia model in vivo", Mol Cell Biochem, 390, pp.143~153 (2014)
- Lee JY, "Hexane fraction from Laminaria japonica exerts anti-inflammatory effects on lipopolysaccharide- stimulated RAW 264.7 macrophages via inhibiting NF-kappaB pathway", Eur J Nutr, 52, pp.409~421 (2013)
- Lee YS, "Antitumor and immunonodulation effects of seaweeds toward sarcoma-180 cell", J Korean Soc Food Nutr, 21, pp.544~550 (1992)
- Makarov SS, "NF-kappa B in rheumatoid arthritis: a pivotal regulator of inflammation, hyperplasia, and tissue destruction", Arthritis Res, 3, pp.200~206 (2001)
- Marks-Konczalik J, "Cytokinemediated transcriptional induction of the human inducible nitric oxide synthase gene requires both activator protein 1 and nuclear factor kappaB-binding sites", J Biol Chem, 273, pp.22201~22208 (1998)
- Nathan C, "Nitric oxide as a secretory product of mammalian cells", FASEB J, 6, pp.3051~3064 (1992)
- "National Fisheries Research & Development Institute" (2009)[URL]
- Nguyen T, "Transcriptional regulation of the antioxidant response element. Activation by Nrf2 and repression by MafK", J Biol Chem, 275, pp.15466~15473 (2000)
- Otterbein LE, "Carbon monoxide provides protection against hyperoxic lung injury", Am J Physiol, 276, pp.L688~L694 (1999)
- Pahan K, "Induction of nitric-oxide synthase and activation of NF-kappaB by interleukin-12 p40 in microglial cells", J Biol Chem, 276, pp.7899~7905 (2001)
- Pahl HL, "Activators and target genes of Rel", NF-kappaB transcription factors, 1999, pp.18, 6853~6866
- Pan MH, "Se-methylselenocysteine inhibits lipopolysaccharide-induced NFkappaB activation and iNOS induction in RAW 264.7 murine macrophages", Mol Nutr Food Res, 55, pp.723~732 (2011)
- 박나비, "애기외톨개모자반 (Myagropsis yendoi) 추출물의 항균활성", 한국수산과학회지, 43(6), pp.642~647 (2010), 한국수산과학회
- Park SY, "Heme oxygenase-1 signals are involved in preferential inhibition of pro-inflammatory cytokine release by surfactin in cells activated with Porphyromonas gingivalis lipopolysaccharide", Chem Biol Interact, 188, pp.437~445 (2010)
- Ryter SW, "Heme oxygenase-1", carbon monoxide: from basic science to therapeutic applications, 2006, pp.86, 583~650
- Xie QW, "Promoter of the mouse gene encoding calcium-independent nitric oxide synthase confers inducibility by interferon gamma and bacterial lipopolysaccharide", J Exp Med, 177, pp.1779~1784 (1993)
- Zhang G, "Molecular mechanisms of NFkappaB activation induced by bacterial lipopolysaccharide through Toll-like receptors", J Endotoxin Res, 6, pp.453~457 (2000)