한국산 수국속(Hydrangea L.) 식물의 분자 계통학적 연구
Molecular Phylogenetic Study of Korean Hydrangea L.
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본 연구는 ITS region과 3개의 엽록체 DNA region을 활용하여 한국산 수국속 7 분류군에 대한 계통학적 유연관계를 규명하고자 수행되었다. 계통학적 분석 결과 한국산 수국속의 7분류군은 단계통군을 형성하였으며, Macrophyllae 아절은 산수국과수국의 두 개의 분계조로 나뉘었다. 산수국의 분계조는 꽃산수국과 탐라산수국이 유집되어 하나의 단계통군을 형성하였으며, 염기서열상으로도 뚜렷한 차이가 나타나지 않았다. 수국의 분계조는 산수국의 분계조와 독립된 분계조를 형성하며 뚜렷이구분되었다. 수국속 분계조 내의 Petalanthe, Heteromallae, Calyptranthae 아절은 단계통군을 형성하였지만, Calyptranthae 아절에 속하는 등수국은 한라산 개체를 제외한 제주도 집단과울릉도, 일본 집단으로 두 개의 소분계조를 이루었다. 등수국의두 개의 소분계조에 대한 추가적인 연구는 더 많은 양의 채집과지리학적인 연구가 추가 되어야 할 것으로 판단된다.
Abstract
In this study, the phylogenetic relationship of Korean Hydrangea was evaluated by using sequenced three chloroplast regions and ITS region, including the 7 taxa. The result of phylogenetic analysis indicated that Korean Hydrangea, 7 taxa formed the monophyletic group. This analysis also revealed that subsect. Macrophyllae of Korea was separated into two groups; H. serrata f. acuminate and H. macrophylla group. The H. serrata f. acuminta group was included with H. serrata f. buergeri and H. serrata f. fertilis. These three species form a monophyletic clade, with no significant differences between their nucleotide sequences. The H. serrata f. acuminta group showed a monophyletic group with H. serrata f. buergeri and H. serrata f. fertilis and there is significant differences between their nucleotide sequences. H. macrophylla group was an independent clade distinguished by H. serrate f. acuminate group. Subsect. Petalanthe, Heteromallae and Calyptranthae form a monophyletic group. H. petiolaris which is located in Subsect. Calyptranthae was separated into two subgroups; First subgroup: Jeju island (except for Mt. Halla) and Second subgroup: Ulleung island and Japan. Additional studies of two subgroups of H. petiolaris should be conducted a geographical study and add more samples. Key words - Calyptranthae, Macrophyllae, Monophyletic, Phylogenetic analysis
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