본문 바로가기
KERD
논문KCI 등재

Screening of Rice Germplasm for the Distribution of Rice Blast Resistance Genes and Identification of Resistant Sources

0

조회

0

다운로드

0

인용

0

참고문헌

키워드

초록

Rice blast, caused by a fungus Magnaporthe oryzae, is one of the most devastating diseases of rice worldwide. Analyzing the valuable genetic resources is important in making progress towards blast resistance. Molecular screening of major rice blast resistance (R) genes was determined in 2,509 accessions of rice germplasm from different geographic regions of Asia and Europe using PCR based markers which showed linkage to twelve major blast R genes, Pik-p, Pi39, Pit, Pik-m, Pi-d(t)2, Pii, Pib, Pik, Pita, Pita/Pita-2, Pi5, and Piz-t. Out of 2,509 accessions, only two accessions had maximum nine blast resistance genes followed by eighteen accessions each with eight R genes. The polygenic combination of three genes was possessed by maximum number of accessions (824), while among others 48 accessions possessed seven genes, 119 accessions had six genes, 267 accessions had five genes, 487 accessions had four genes, 646 accessions had two genes, and 98 accessions had single R gene. The Pik-p gene appeared to be omnipresent and was detected in all germplasm. Furthermore, principal component analysis (PCA) indicated that Pita, Pita/Pita-2, Pi-d(t)2, Pib and Pit were the major genes responsible for resistance in the germplasm. The present investigation revealed that a set of 68 elite germplasm accessions would have a competitive edge over the current resistance donors being utilized in the breeding programs. Overall, these results might be useful to identify and incorporate the resistance genes from germplasm into elite cultivars through marker assisted selection in rice breeding. Key words - Rice blast, Disease resistance, Rice germplasm, Resistance gene

Abstract

Rice blast, caused by a fungus Magnaporthe oryzae, is one of the most devastating diseases of rice worldwide. Analyzing the valuable genetic resources is important in making progress towards blast resistance. Molecular screening of major rice blast resistance (R) genes was determined in 2,509 accessions of rice germplasm from different geographic regions of Asia and Europe using PCR based markers which showed linkage to twelve major blast R genes, Pik-p, Pi39, Pit, Pik-m, Pi-d(t)2, Pii, Pib, Pik, Pita, Pita/Pita-2, Pi5, and Piz-t. Out of 2,509 accessions, only two accessions had maximum nine blast resistance genes followed by eighteen accessions each with eight R genes. The polygenic combination of three genes was possessed by maximum number of accessions (824), while among others 48 accessions possessed seven genes, 119 accessions had six genes, 267 accessions had five genes, 487 accessions had four genes, 646 accessions had two genes, and 98 accessions had single R gene. The Pik-p gene appeared to be omnipresent and was detected in all germplasm. Furthermore, principal component analysis (PCA) indicated that Pita, Pita/Pita-2, Pi-d(t)2, Pib and Pit were the major genes responsible for resistance in the germplasm. The present investigation revealed that a set of 68 elite germplasm accessions would have a competitive edge over the current resistance donors being utilized in the breeding programs. Overall, these results might be useful to identify and incorporate the resistance genes from germplasm into elite cultivars through marker assisted selection in rice breeding. Key words - Rice blast, Disease resistance, Rice germplasm, Resistance gene

참고문헌 (45)

  1. Abe, S., "Breeding of a blast resistant multiline variety of rice, Sasanishiki BL", Jpn. Agr. Res. Q, 38, pp.149~154 (2004)
  2. Ashikawa, I., "Two adjacent nucleotide binding site-leucine-rich repeat class genes are required to confer Pikm-specific rice blast resistance", Genetics, 180(4), pp.2267~2276 (2008)
  3. Ballini, E., "A genome-wide meta-analysis of rice blast resistance genes and quantitative trait loci provides new insights into partial and complete resistance", Mol. Plant Microbe In, 21, pp.859~868 (2008)
  4. Chen, X. W., "A B-lectin receptor kinase gene conferring rice blast resistance", Plant J, 46, pp.794~804 (2006)
  5. Chen, X. W., "Identification of two blast resistance genes in a rice variety", Digu. J. Phytopathol, 152, pp.77~85 (2004)
  6. Young-Chan Cho, "Haplotype Diversity and Durability of Resistance Genes to Blast in Korean Japonica Rice Varieties", Journal of Crop Science and Biotechnology, 11(3), pp.205~214 (2008), 한국작물학회
  7. 조영찬, "Identification of Major Blast Resistance Genes in Korean Rice Varieties (Oryza sativa L.) Using Molecular Markers", Journal of Crop Science and Biotechnology, 10(4), pp.265~276 (2007), 한국작물학회
  8. Gamuyao, R., "The protein kinase Pstol1from traditional rice confers tolerance of phosphorus deficiency", Nature, 488, pp.535~541 (2012)
  9. Han, S. S., "Breakdown of resistant cultivars by new race KI-1117a and race distribution of rice blast fungus during 1999-2000 in Korea", Res. Plant Dis, 7, pp.86~92 (2001)
  10. Hayashi, K., "Refunctionalization of the ancient rice blast disease resistance gene Pit by the recruitment of a retrotransposon as a promoter", Plant J, 57(3), pp.413~425 (2009)[DOI]
  11. Hayashi, K., "Development of PCR-based allele-specific and InDel marker sets for nine rice blast resistance genes", Theor. Appl. Genet, 113, pp.251~260 (2006)
  12. Hayashi, K., "Development of PCR-based SNP markers for rice blast resistance genes at the Piz locus", Theor. Appl. Genet, 108(7), pp.1212~1220 (2004)
  13. Higashi, T., "Linkage of field resistance genes of upland rice variety ‘Sensho’ to leaf blast caused by Pyricularia oryzae Cav", Jpn. J. Breed, 35, pp.438~448 (1985)
  14. Imam, J., "Molecular screening for identification of blast resistance genes in North East and Eastern Indian rice germplasm(Oryza sativa L.)with PCR based makers", Euphytica, 196, pp.199~211 (2014)
  15. J. U. Jeung, "A novel gene, Pi40(t), linked to the DNA markers derived from NBS-LRR motifs confers broad spectrum of blast resistance in rice", Theoretical and Applied Genetics, 115(8), pp.1163~1177 (2007), Springer Nature[DOI]
  16. "A user-friendly method to isolate and single spore the fungi Magnaporthe oryzae and Magnaporthe grisea obtained from diseased field samples Plant Health Prog" (2009)[URL]
  17. Jia, Y., "Development of dominant rice blast Pi-ta resistance gene markers", Crop Sci, 42, pp.2145~2149 (2002)
  18. Kelly, J. D., "Use of RAPD markers in breeding for major gene resistance to plant pathogens", Hortic. Sci, 30, pp.461~465 (1995)
  19. Khush, G. S., "Harnessing science and technology for sustainable rice-based production systems. Proceedings of FAO Rice Conference “Rice is life”", Int. Rice Comm. Newsl, 53, pp.17~23 (2004)
  20. Khush, G. S., "Advances in Genetics, Genomics and Control of Rice Blast Disease", pp.1~10 (2009), Springer
  21. 김정순, "Screening of Rice Blast Resistance Genes from Aromatic Rice Germplasms with SNP Markers", The Plant Pathology Journal, 26(1), pp.70~79 (2010), 한국식물병리학회
  22. Kiyosawa, S., "Studies on genetics and breeding of blast resistance in rice", Misc. Publ. Natl. Inst. Agric. Sci, 1, pp.1~58 (1974)
  23. Kiyosawa, S., "With genetic view on the mechanism of resistance and virulence", Jpn. J. Iden, 41, pp.89~92 (1987)
  24. Lee, S. K., "Rice Pi5-mediated resistance to Magnaporthe oryzae requires the presence of two coiled-coil-nucleotide-binding-leucine-rich repeat genes", Genetics, 181(4), pp.1627~1638 (2009)
  25. Li, Y. B., "Dynamic analyses of rice blast resistance for the assessment of genetic and environmental effects", Plant Breed, 126, pp.541~547 (2007)
  26. Jinbin Li, "Genetic variation and evolution of the Pit blast resistance locus in rice", Genetic Resources and Crop Evolution, 61(2), pp.473~489 (2014), Springer Nature[DOI]
  27. Liu G., "Two broad-spectrum blast resistance genes, Pi9 ( t ) and Pi2 ( t ), are physically linked on rice chromosome 6", Molecular Genetics and Genomics, 267(4), pp.472~480 (2002), Springer Nature[DOI]
  28. Xinqiong Liu, "Identification and fine mapping of Pi39(t), a major gene conferring the broad-spectrum resistance to Magnaporthe oryzae", Molecular Genetics and Genomics, 278(4), pp.403~410 (2007), Springer Nature[DOI]
  29. Padmanabhan, S. Y., "Estimating losses from rice blast in India. Pages 203-221 in the rice blast disease", Proceeding of symposium at IRRI (1965), The Johns hopkin press
  30. Pink, D. A. C., "Strategies using genes for non-durable resistance", Euphytica, 1, pp.227~236 (2002)
  31. Qu, S., "The broad-spectrum blast resistance gene Pi9 encodes a nucleotide-binding site–leucine-rich repeat protein and is a member of a multigene family in rice", Genetics, 172, pp.1901~1914 (2006)
  32. Rath, G., "Cytoplasmic effect on the leaf blast reaction in rice", Curr. Sci, 41, pp.338~339 (1972)
  33. Shankar, R., "Diversity analysis of bitter gourd(Momordica charantia L. )germination from tribal belts of India", Asian & Australian J. Plant Sci. Biotech, pp.21~25 (2009)
  34. Sharma, T. R., "Rice blast management through host-plant resistance : retrospect and prospects", Agric. Res, 1, pp.37~52 (2012)
  35. Shikari, A. B., "Molecular analysis and phenotypic validation of blast resistance genes Pita and Pita2 in landraces of rice(Oryza sativa L.)", Indian J. Genet, 73, pp.131~141 (2013)
  36. Song, C., "The classification of some species of rice in Heilongjiang province based on its resistance to the blast", Chin. Agric. Sci. Bull, 25, pp.201~205 (2009)
  37. 송재영, "Blast Resistant Genes Distribution and Resistance Reaction to Blast in Korean Landraces of Rice (Oryza sativa L.)", 한국자원식물학회지, 27(6), pp.687~700 (2014), 한국자원식물학회[DOI]
  38. Tanksley, S. D., "Seeds banks and molecular maps : unlocking genetic potential from the wild", Science, 277, pp.1063~1066 (1997)
  39. Tsunematsu, H., "Development of monogenic lines of rice for blast resistance", Breed. Sci, 50, pp.229~234 (2000)
  40. Variar, M., "Advances in Genetics, Genomics and Control of Rice Blast Disease", pp.359~374 (2009), Springer
  41. Wang, G. L., "RFLP mapping of genes conferring complete and partial resistance to blast in a durably resistant rice cultivar", Genetics, 136, pp.1421~1434 (1994)
  42. Wang, L., "Characterization of rice blast resistance genes in the Pik cluster and Wne mapping of the Pik-p locus", Phytopathology, 99, pp.900~905 (2009)
  43. Yang, J. Y., "Race specificity of major rice blast resistance genes to Magnaporthe grisea isolates collected from indica rice in Guangdong, China", Rice Sci, 15, pp.311~318 (2008)
  44. Zhai, C., "The isolation and characterization of Pik, a rice blast resistance gene which emerged after rice domestication", New Phytol, 189, pp.321~334 (2011)
  45. Zhu, Y., "Genetic diversity and disease control in rice", Nature, 406, pp.718~722 (2000)

관련 논문AI추천

Blast Resistant Genes Distribution and Resistance Reaction to Blast in Korean Landraces of Rice (Oryza sativa L.)박홍재, 배창휴, 송재영, 이광범, 이기안, 이명철, 이수경, 정연주, 최유미, 현도윤한국자원식물학회 · 한국자원식물학회지2014 · 제27권 제6호벼 단인자 계통과 우량 벼품종의 도열병 저항성 분석을 통한 안정 저항성 유전자 탐색박현수, 백만기, 안억근, 이점호, 이지윤, 이창민, 이효정, 정오영, 정종민, 정지웅, 조영찬, 현웅조한국육종학회 · 한국육종학회지2021 · 제53권 제3호Analysis of Korean japonica rice cultivars using molecular markers associated with blast resistance genesJae-Hwan Roh, Jung-Pil Suh, Kyung-Ho Kang, Seong-Sook Han, Yeon-Gyu Kim, Yong-Hee Jeon, Young-Chan Cho한국육종학회 · 한국육종학회지2008 · 제40권 제3호Genetic Diversity of Blast Resistance Donors and Selected Korean Rice Cultivars Based on Simple Sequence Repeats안상낙, 한성숙, Hae-Chune Choi, Hung-Goo Hwang, Hyun-Jung Kim, Soo-Jin Kwon한국육종학회 · 한국육종학회지2003 · 제35권 제3호국내 벼 품종의 도열병 저항성 유전자 유전형 분석박현수, 이창민, 이현숙, 정지웅, 하수경한국육종학회 · 한국육종학회지2022 · 제54권 제4호벼 도열병 저항성 유전자 Pi45(t)의 균계 특이적 반응과 고밀도지도 작성구홍광, 김동민, 노재환, 안상낙, 한성숙, 양바오로한국육종학회 · 한국육종학회지2011 · 제43권 제1호

이 간행물의 다른 논문 (9건)