본문 바로가기
KERD
논문KCI 등재

벼 단인자 계통과 우량 벼품종의 도열병 저항성 분석을 통한 안정 저항성 유전자 탐색

Identification of Stable Resistance Genes Based on Resistance Evaluation to Blast for Monogenic Lines and Leading Japonica Varieties in Rice

2

조회

0

다운로드

3

인용

0

참고문헌

키워드

주제분류농학

초록

Rice is the most important staple food in Korea. Rice blast, caused by Magnaporthe oryzae, is one of the most devastating diseasesin the rice-growing world and in Korea. Eighteen major blast resistance genes were screened in 33 leading Korean japonica varieties using44 DNA markers. The 33 leading japonica varieties are cultivated in 659,642 ha (90.4%), and a variety of Samkwang is cultivated widelyon a nation-wide scale. Four resistance genes, Pia, Pib, Pi19, and Pb1, were detected in 10-21 varieties, and the R-genes of Pii, Pish, Pita/Pita-2,Piz/Piz-t, Pi35, and Pi65(t), were present in 2-5 varieties. Five R-genes, Pit, Pi5, Pi9, pi21, and Pi40, were not detected in the Korean japonicarice varieties. A total of six varieties, two mid-late flowering varieties, Ilmi and Saeilmi, a medium flowering variety Gopum; and three earlyflowering varieties Jopyeong, Haedamssal, and Haedeul; were resistant to blast nursery screening over 12 years. A variety of Gopum harboredalleles for Piz/Piz-t, and three early flowering varieties, Jopyeong, Haedamssal, and Haedeul, had extensive loci of the multiple-gene familyPiz/Piz-t, Pi9, and Pi40 on chromosome 6. Two mid-late flowering varieties, Ilmi and Saeilmi, harbored the Pita-2 gene, and Gopum hadPita. In the japonica rice breeding program, an effective means for enhancing stable resistance to blast will be to introduce resistance genesPit, Pi5, Pi9, pi21 and Pi40 which were not identified in Korean japonica rice varieties, and resistance genes Pita/Pita-2, Piz-t/Piz-5, to 2-3R genes among and Pi20 that showed stable resistance in monogenic IRBL lines.

Abstract

Rice is the most important staple food in Korea. Rice blast, caused by Magnaporthe oryzae, is one of the most devastating diseasesin the rice-growing world and in Korea. Eighteen major blast resistance genes were screened in 33 leading Korean japonica varieties using44 DNA markers. The 33 leading japonica varieties are cultivated in 659,642 ha (90.4%), and a variety of Samkwang is cultivated widelyon a nation-wide scale. Four resistance genes, Pia, Pib, Pi19, and Pb1, were detected in 10-21 varieties, and the R-genes of Pii, Pish, Pita/Pita-2,Piz/Piz-t, Pi35, and Pi65(t), were present in 2-5 varieties. Five R-genes, Pit, Pi5, Pi9, pi21, and Pi40, were not detected in the Korean japonicarice varieties. A total of six varieties, two mid-late flowering varieties, Ilmi and Saeilmi, a medium flowering variety Gopum; and three earlyflowering varieties Jopyeong, Haedamssal, and Haedeul; were resistant to blast nursery screening over 12 years. A variety of Gopum harboredalleles for Piz/Piz-t, and three early flowering varieties, Jopyeong, Haedamssal, and Haedeul, had extensive loci of the multiple-gene familyPiz/Piz-t, Pi9, and Pi40 on chromosome 6. Two mid-late flowering varieties, Ilmi and Saeilmi, harbored the Pita-2 gene, and Gopum hadPita. In the japonica rice breeding program, an effective means for enhancing stable resistance to blast will be to introduce resistance genesPit, Pi5, Pi9, pi21 and Pi40 which were not identified in Korean japonica rice varieties, and resistance genes Pita/Pita-2, Piz-t/Piz-5, to 2-3R genes among and Pi20 that showed stable resistance in monogenic IRBL lines.

참고문헌 (55)

  1. Ahn SW, "Blast reaction of durably resistance rice cultivar in multiflication trials", Phytopathology, 81, pp.1150~ (1991)
  2. Angeles-Shim RB, "Markerassisted introgression of quantitative resistance gene pi21confers broad-spectrum resistance to rice blast", Sci Direct, 27, pp.113~123 (2020)
  3. Ashikawa I, "Two adjacent nucleotidebinding site-leucine-rich repeat class genes are requaired to confer Pikm-specific rice blast resistance", Genetics, 180, pp.2267~2276 (2008)
  4. Ashkani S, "Molecular progress on the mapping and cloning of functional genes for blast disease in rice (Oryza sativa L.): Current status and future considerations", Crit Rev Biotechnol, 36, pp.353~367 (2016)
  5. Cho YC, "‘Suweon545’ harboring QTL related to blast resistance in Korean weedy rice", Korean J Breed Sci, 42(Suppl. 1), pp.138~ (2010)
  6. 조영찬, "Identification of Major Blast Resistance Genes in Korean Rice Varieties (Oryza sativa L.) Using Molecular Markers", Journal of Crop Science and Biotechnology, 10(4), pp.265~276 (2007), 한국작물학회
  7. Young-Chan Cho, "Reaction of Resistance Genes of Monogenic Lines to Rice Blast (Magnaporthe grisea)", 한국육종학회지, 37(3), pp.155~161 (2005), 한국육종학회
  8. 최임수, "A Medium-Maturing, Multi-Disease Resistant and Good Eating-Quality Rice Variety “Gopumbyeo”", 한국육종학회지, 39(4), pp.586~587 (2007), 한국육종학회
  9. Costanzo S, "Sequence variation at the rice blast resistance gene Pi-km locus : Implications for the development of alleles specific markers", Plant Sci, 178, pp.523~530 (2010)
  10. Chukwu SC, "Marker-assisted introgression of multiple resistance genes confers broad spectrum resistance against bacterial leaf blight and blast diseases in PUTRA-1 rice variety", Agronomy, 10, pp.42~ (2020)
  11. Dellaporta SL, "A plant DNA mini preparation, Version II", Plant Mol Biol Rep, 1, pp.19~21 (1983)
  12. Deng Y, "Genetic characterization and fine mapping of the blast resistance locus Pigm(t)tightly linked to Pi2 and Pi9 in a broad-spectrum resistant Chinese variety", Theor Appl Genet, 113, pp.705~713 (2006)
  13. Fjellstrom R, "Development of DNA markers suitable for marker assisted selection of three Pi genes conferring resistance to multiple Pyricularia grisea pathotypes", Crop Sci, 44, pp.1790~1798 (2004)
  14. Fujii K, "Identification of a RFLP marker tightly linked to the panicle blast resistance gene, Pb1, in rice", Breed Sci, 50, pp.183~188 (2000)
  15. Fukuoka S, "QTL analysis and mapping of pi21, a recessive gene for field resistance to rice blast in Japanese upland rice", Theor Appl Genet, 103, pp.185~190 (2001)
  16. Gandeka M, "Developing rice restorer lines resistance to bacterial blight, blast and brown plant hopper by molecular pyramiding", Intl J Agrono & Agricul Res, 7, pp.155~165 (2015)
  17. Han SS, "Analysis of rapid increase of rice blast fungus race KI-409 in Korea", Korean J Plant Pathol, 14, pp.705~709 (1998)
  18. Han SS, "Breakdown of resistant cultivars by new race KI-1117a and race distribution of rice blast fungus during 1999~2000 in Korea", Res. Plant Dis, 7, pp.86~92 (2001)
  19. Hayashi K, "Development of PCR-based SNP markers for rice blast resistance genes at the Piz locus", Theor Appl Genet, 108, pp.1212~1220 (2004)
  20. Hayashi K, "Identification of the blast resistance gene Pit in rice cultivars using functional markers", Theor Appl Genet, 121, pp.1357~1367 (2010)
  21. Hua L, "The isolation of Pi1, an allele at the Pik locus which confers broad spectrum resistance to rice blast", Theor Appl Genet, 125, pp.1047~1055 (2012)
  22. Jeung JU, "A novel gene, Pi40(t), linked to the DNA markers derived from NBS-LRR motif confers broad spectrum of blast resistance in rice", Theor Appl Genet, 115, pp.1163~1177 (2007)
  23. Jia Y, "Rice Pi-ta gene confers resistance to the major pathotypes of the rice balst fungus in the United States", Phytopathology, 94, pp.296~301 (2004)
  24. Khush GS, "What it will take to feed 5. 0 billion rice consumers in 2030", Plant Mol Biol, 59, pp.1~6 (2005)
  25. Kitazawa N, "Rapid DNA-genotyping system targeting ten loci for resistance to blast disease in rice", Breeding Sci, 69, pp.68~83 (2019)
  26. Kobayashi A, "Koshihikari : A premium short-grain rice cultivar-its expansion and breeding in Japan", Rice, 11, pp.15~ (2018)
  27. Koide Y, "Resistance genes and selection DNA markers for blast disease in rice (Oryza sativa L.)", Jpn Agric Res Q, 41, pp.31~37 (2009)
  28. 이종희, "Enhancement of Panicle Blast Resistance in Korean Rice Cultivar ‘Saeilmi’ by Marker Assisted Backcross Breeding", Plant Breeding and Biotechnology, 3(1), pp.1~10 (2015), 한국육종학회[DOI]
  29. Liu J, "Recent progress and understanding of the molecular mechanisms of the rice-Magnaporthe oryzae interaction", Mol Plant Pathol, 11, pp.419~427 (2010)
  30. Notteghem JL, "Durability of disease resistance", pp.125~134 (1993), Kluwer Academic Publishers
  31. Okuyama Y, "A multifaceted genomics approach allows the isolation of the rice Pia-blast resistance gene consisting of two adjacent NBS-LRR protein genes", Plant J, 66, pp.467~479 (2011)
  32. Pradhan SK, "Pyramiding of three bacterial blight resistance genes for broad-spectrum resistance in deep water rice variety, Jalmagna", Rice, 8, pp.1~14 (2015)
  33. Qu S, "The broad-spectrum blast resistance gene Pi9 encodes a nucleotide-binding site-leucine-rich repeat protein and is a member of a multigene family in rice", Genetics, 172, pp.1901~1914 (2006)
  34. Ramkumar G, "Development and validation of functional marker targeting an InDel in the major rice blast disease resistance gene Pi54 (Pikh)", Mol Breeding, 27, pp.129~135 (2011)
  35. 2017 Result Report of New Variety Deliberate Council for Official Development in Agriculture Crop, "Rural Development Administration", RDA, 43~52 (2017)
  36. Savary S, "The global burden of pathogens and pests on major food crops", Nat Ecol, 3, pp.430~439 (2019)
  37. Skamnioti P, "Against the grain : Safeguarding rice from rice blast disease", Trends Biotechnol, 27, pp.141~150 (2009)
  38. 심홍식, "벼 품종 및 지역에 따른 도열병균 레이스 분리와 도열병 발병정도와의 관계", 식물병 연구, 18(4), pp.324~330 (2012), 한국식물병리학회[DOI]
  39. Su J, "Development of specific DNA markers for detecting the rice blast resistance gene alleles Pi2", 9, Agricultural Biotechnology, pp.2020, 9[DOI]
  40. Suh JP, "Development of breeding lines with three pyramided resistance genes that confer broad-spectrum bacterial blight resistance and their molecular analysis in rice", Rice, 6, pp.5~ (2013)
  41. Sun LT, "Effects of multiple genes polymerization on rice blast resistance in Jiangsu Province and the development of functional markers of Pb1 gene", Journal of Southern Agriculture, 50, pp.913~923 (2019)
  42. Takahashi A, "Unique features of the rice blast resistance Pish locus revealed by large scale retrotransposon-tagging", BMC Plant Biol, 10, pp.175~ (2010)
  43. Tsunematsu H, "Development of monogenic lines of rices for blast resistance", Breed Sci, 50, pp.229~234 (2000)
  44. Wang X, "Current advances on genetic resistance to rice blast disease, rice germplasm", Genetics and Improvement, pp.195~217 (2014)
  45. Wu Y, "Combination patterns of major R genes determine the level of resistance to the M. oryzae in rice (Oryza sativa L.)", PLoS ONE, 10, pp.e0126130~ (2015)
  46. Xu XW, "Advances in molecular markers of blast resistance genes and marker-assisted breeding rice", Jiangsu J Agricul Sci, 29, pp.403~410 (2013)
  47. Yamada M, "Break down of rice blast disease in Korea", Ann Phytopathol Soc Jpn, 32, pp.14~19 (1978)
  48. Yi G, "Use of Pi5(t) markers in marker-assisted selection to screen for cultivars with resistance to Magnaporthe grisea", Theor Appl Genet, 109, pp.978~985 (2004)
  49. Yokoo M, "Introduction of Piz-t and Pib genes for blast disease resistance from indica varieties and their utilization for genetic researches in rice", Japanese Agri Res Quarterly, 39, pp.239~245 (2005)
  50. Yu M, "Resistance spectrum difference between two broadspectrum blast resistance genes, Pigm and Pi2, and their interaction effect on Pi1", Acta Agron Sin, 39, pp.1927~1934 (2013)
  51. Yuan B, "The Pik-p resistance to Magnaporthe oryzae in rice is mediated by a pair of closely linked CC-NBS-LRR genes", Theor Appl Genet, 122, pp.1017~1028 (2011)
  52. Zeng S, "Development of specific markers for Pigm in marker-assisted breeding of panicle blast resistant japonica rice", Chin J Rice Sci, 32, pp.453~461 (2018)
  53. Zhai C, "The isolation and characterization of Pik, a rice blast resistance gene which emerged after rice domestication", New Phytol, 189, pp.321~334 (2011)
  54. Zhao H, "The rice blast resistance gene Ptr encodes an atypical protein required for broad-spectrum disease resistance", Nat Commun, 9, pp.2039~ (2018)
  55. Zheng W, "Genetic mapping and molecular marker development for Pi65(t), a novel broad-spectrum resistance gene to rice blast using next-generation sequencing", Theor Appl Genet, 129, pp.1035~1044 (2016)

관련 논문AI추천

국내 벼 품종의 도열병 저항성 유전자 유전형 분석박현수, 이창민, 이현숙, 정지웅, 하수경한국육종학회 · 한국육종학회지2022 · 제54권 제4호Blast Resistant Genes Distribution and Resistance Reaction to Blast in Korean Landraces of Rice (Oryza sativa L.)박홍재, 배창휴, 송재영, 이광범, 이기안, 이명철, 이수경, 정연주, 최유미, 현도윤한국자원식물학회 · 한국자원식물학회지2014 · 제27권 제6호Analysis of Korean japonica rice cultivars using molecular markers associated with blast resistance genesJae-Hwan Roh, Jung-Pil Suh, Kyung-Ho Kang, Seong-Sook Han, Yeon-Gyu Kim, Yong-Hee Jeon, Young-Chan Cho한국육종학회 · 한국육종학회지2008 · 제40권 제3호“금오3호”의 벼 잎도열병 저항성 특성 및 저항성 연관 마커 탐색강종래, 곽도연, 김춘송, 노재환, 박동수, 신문식, 여운상, 오병근, 이기환, 이종희, 전명기, 황흥구한국육종학회 · 한국육종학회지2008 · 제40권 제4호Screening of Rice Germplasm for the Distribution of Rice Blast Resistance Genes and Identification of Resistant Sources박홍재, 오세종, 이명철, 이수경, 최유미, 현도윤, Asjad Ali한국자원식물학회 · 한국자원식물학회지2016 · 제29권 제6호벼 도열병 저항성 유전자 Pi45(t)의 균계 특이적 반응과 고밀도지도 작성구홍광, 김동민, 노재환, 안상낙, 한성숙, 양바오로한국육종학회 · 한국육종학회지2011 · 제43권 제1호

이 간행물의 다른 논문 (14건)