The Protective and Inhibitory Effect of Antioxidants Found in Broussonetia kazinoki Siebold against Oxidative DNA Damage
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Oxidative DNA damage negatively affects humans and the research is currently ongoing to find ways to reduce oxidative stress. Oxidative stress has been identified as a key factor in triggering various diseases. Thus, its alleviation is important for human health. Broussonetia kazinoki (B. kazinoki) has been used in traditional Korean medicine as a dermatological therapy to treat burns, pruritus, and acne. B. kazinoki is generally segregated into peeled root (PR), root bark (RB), peeled stem (PS), and stem bark (SB). To assess these components for their antioxidant activity and protection against DNA damage, their ethyl acetate fractions were examined by 1,1-diphenyl-2-picryl hydrazyl (DPPH) and 2,2'-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical scavenging assay. As a result of confirming the expression of factors involved in attenuating DNA damage, the protective effect of SB on oxidative stress suppressed the expression of p-p53 and γ-H2AX. Additionally, the levels of p53 and H2AX mRNA were significantly downregulated. In conclusion, these results indicated that the SB component of B. kazinoki had the potential to be used as an effective natural antioxidant compared to the other parts of the plant.
Abstract
Oxidative DNA damage negatively affects humans and the research is currently ongoing to find ways to reduce oxidative stress. Oxidative stress has been identified as a key factor in triggering various diseases. Thus, its alleviation is important for human health. Broussonetia kazinoki (B. kazinoki) has been used in traditional Korean medicine as a dermatological therapy to treat burns, pruritus, and acne. B. kazinoki is generally segregated into peeled root (PR), root bark (RB), peeled stem (PS), and stem bark (SB). To assess these components for their antioxidant activity and protection against DNA damage, their ethyl acetate fractions were examined by 1,1-diphenyl-2-picryl hydrazyl (DPPH) and 2,2'-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical scavenging assay. As a result of confirming the expression of factors involved in attenuating DNA damage, the protective effect of SB on oxidative stress suppressed the expression of p-p53 and γ-H2AX. Additionally, the levels of p53 and H2AX mRNA were significantly downregulated. In conclusion, these results indicated that the SB component of B. kazinoki had the potential to be used as an effective natural antioxidant compared to the other parts of the plant.
참고문헌 (44)
- Alvarez-Idaboy, J. R., "A. On the chemical repair of DNA radicals by glutathione : Hydrogen vs. electron transfer", J. Phys. Chem. B, 116(31), pp.9316~9325 (2012)
- Official Methods of Analysis, "AOAC", Association of Official Analytical Chemists, 8~35 (1995)
- Attaguile, G., "Antioxidant activity and protective effect on DNA cleavage of extracts from Cistus incanus L. and Cistus monspeliensis L", Cell Biol Toxicol, 16(2), pp.83~90 (2000)
- Bae, U. J., "A prenylated flavan from Broussonetia kazinoki prevents cytokine-induced β-cell death through suppression of nuclear factor-κB activity", Biol. Pharm. Bull, 34(7), pp.1026~1031 (2011)
- Baek, Y. S., "Tyrosinase inhibitory effects of 1, 3-diphenylpropanes from Broussonetia kazinoki", Bioorg. Med. Chem, 17(1), pp.35~41 (2009)
- Bellon, S., "Cross-linked thymine-purine base tandem lesions : Synthesis, characterization, and measurement in γ-irradiated isolated DNA", Chem. Res. Toxicol, 15(4), pp.598~606 (2002)
- Bondet, V., "Kinetics and mechanisms of antioxidant activity using the DPPH free radical method", LWT Food Sci. Technol, 30(6), pp.609~615 (1997)
- Box, H. C., "Free radical-induced tandem base damage in DNA oligomers", Free Radic. Biol. Med, 23(7), pp.1021~1030 (1997)
- Circu, M. L., "Reactive oxygen species, cellular redox systems, and apoptosis", Free Radic. Biol. Med, 48(6), pp.749~762 (2010)
- Crean, C., "Oxidation of single-stranded oligonucleotides by carbonate radical anions : Generating intrastrand cross-links between guanine and thymine bases separated by cytosines", Nucleic Acids Res, 36(3), pp.742~755 (2008)
- Dinstel, R. R., "The antioxidant level of Alaska’s wild berries: High, higher and highest", Int. J. Circumpolar Health, 72 (2013)
- Fernandez-Capetillo, O., "H2AX: the histone guardian of the genome", DNA Repair, 3(8-9), pp.959~967 (2004)
- Galano, A., "Melatonin and its metabolites as copper chelating agents and their role in inhibiting oxidative stress : a physicochemical analysis", J. Pineal Res, 58(1), pp.107~116 (2015)
- 한혜민, "마름 추출물의 항산화활성 및 암세포증식 억제 활성", 한국약용작물학회지, 24(1), pp.14~20 (2016), 한국약용작물학회[DOI]
- 장태원, "Antioxidant Activity and Inhibitory Effects on Oxidative DNA Damage of Callus from Abeliophyllum distichum Nakai", 한국자원식물학회지, 31(3), pp.228~236 (2018), 한국자원식물학회[DOI]
- 김태현, "A549세포에서 닥나무 추출물의 미토콘드리아", Caspase 경로를 통한 Apoptosis 유도작용, 2016, pp.30(3), 150~156[DOI]
- Kong, Y. J., "Antimicrobial activity of Quercus mongolica leaf ethanol extract and organic acids against food-borne microorganisms", Kor. J. Food Sci. Technol, 33(2), pp.178~183 (2001)
- Ho Young Lee, "The Leaves of Broussonetia kazinoki Siebold Inhibit Atopic Dermatitis-Like Response on Mite Allergen-Treated Nc", Nga Mice, 2014, pp.22(5), 438~444[DOI]
- Lee, H. J., "Antioxidant components from Broussonetia kazinoki", J. Pharm. Soc. Kor, 41(4), pp.439~443 (1997)
- Lee, T. B, "Coloured Flora of Korea", pp.223~223 (2003), Hayangmunsa
- Leinisch, F., "Peroxyl radical-and photo-oxidation of glucose 6-phosphate dehydrogenase generates cross-links and functional changes via oxidation of tyrosine and tryptophan residues", Free Radic. Biol. Med, 112, pp.240~252 (2017)
- Maréchal, A., "DNA damage sensing by the ATM and ATR kinases", Cold Spring Harb. Perspect. Biol, 5(9), pp.a012716~ (2013)[DOI]
- Maxwell, S. R. J, "Prospects for the use of antioxidant therapies", Drugs, 49(3), pp.345~361 (1995)
- Meir, S., "Determination and involvement of aqueous reducing compounds in oxidative defense systems of various senescing leaves", J. Agric. Food Chem, 43(7), pp.1813~1819 (1995)
- Minko, I. G., "Chemistry and biology of DNA containing 1, N 2-deoxyguanosine adducts of the α, β-unsaturated aldehydes acrolein, crotonaldehyde, and 4-hydroxynonenal", Chem. Res. Toxicol, 22(5), pp.759~778 (2009)
- Mustafa, R., "Total phenolic compounds, flavonoids, and radical scavenging activity of 21 selected tropical plants, J", J. Food Sci, 75(1), pp.C28~C35 (2010)
- Paull, T. T., "A critical role for histone H2AX in recruitment of repair factors to nuclear foci after DNA damage", Curr. Biol, 10(15), pp.886~895 (2000)
- Pisoschi, A. M., "Total antioxidant capacity of some commercial fruit juices : electrochemical and spectrophotometrical approaches", Molecules, 14(1), pp.480~493 (2009)
- Prasad, K. N., "Antioxidant capacities of peel, pulp, and seed fractions of Canarium odontophyllum Miq. fruit", J. Biomed. Biotechnol, 8 (2010)
- Que, F., "Antioxidant activities of five Chinese rice wines and the involvement of phenolic compounds", Food Res. Int, 39(5), pp.581~587 (2006)
- Reiter, R. J., "Neurotoxins : free radical mechanisms and melatonin protection", Curr. Neuropharmacol, 8(3), pp.194~210 (2010)
- Reiter, R. J., "Melatonin : exceeding expectations", Physiology, 29(5), pp.325~333 (2014)
- Roos, W. P., "DNA damage and the balance between survival and death in cancer biology", Nat. Rev. Cancer, 16(1), pp.20~33 (2016)
- Sablina, A. A., "The antioxidant function of the p53 tumor suppressor", Nat. Med, 11(12), pp.1306~1313 (2005)
- Sen, S., "Free radicals, antioxidants, diseases and phytomedicines : current status and future prospect, Int", Int, J. Pharm. Sci. Rev. Res, 3(1), pp.91~100 (2010)
- Shibata, A., "DNA double-strand break repair in a cellular context", Clin. Oncol, 26(5), pp.243~249 (2014)
- Uvaydov, Y., "Generation of guanine-amino acid cross-links by a free radical combination mechanism", PCCP, 16(23), pp.11729~11736 (2014)
- Van den Berg, R., "Applicability of an improved Trolox equivalent antioxidant capacity(TEAC)assay for evaluation of antioxidant capacity measurements of mixtures", Food Chem, 66(4), pp.511~517 (1999)
- Valko, M., "Metals, toxicity and oxidative stress", Curr. Med. Chem, 12(10), pp.1161~1208 (2005)
- Wiseman, H., "Damage to DNA by reactive oxygen and nitrogen species : role in inflammatory disease and progression to cancer", Biochem. J, 313(1), pp.17~29 (1996)
- Yoo, I. D., "Development of new natural antioxidants for cosmeceuticals", J. Soc. Cosmet. Sci. Kor, 31(4), pp.349~357 (2005)
- Yu, Y., "Occurrence, biological consequences, and human health relevance of oxidative stress-induced DNA damage", Chem. Res. Toxicol, 29(12), pp.2008~2039 (2016)
- Zhang, Q., "Independent generation of 5-(2’-deoxycytidinyl)methyl radical and the formation of a novel cross-link lesion between 5-methylcytosine and guanine", J. Am. Chem. Soc, 125(42), pp.12795~12802 (2003)
- Zeng, Y., "UVB-induced formation of intrastrand cross-link products of DNA in MCF-7 cells treated with 5-bromo-2’-deoxyuridine", Biochemistry, 46(27), pp.8189~8195 (2007)
