Protective effects of Betula platyphylla var. japonica extracts against the cellular damage induced by reactive oxygen species
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In our present study, total methanol extracts preparedfrom B. platyphylla var. japonica showed a significantincrease in cell proliferation upon the induction of oxidativestress by hydrogen peroxide or γ-ray irradiation. Totalmethanol extracts were fractionated into five separatepreparations i.e. n-hexane, dichloromethane, ethylacetate,n-butanol and water fractions. Among these, theethylacetate and butanol fractions of B. platyphylla var. japonica showed the highest protective effects againstoxidative stress induced by hydrogen peroxide. Thesefractions also showed strong protective effects against γ-rayirradiation. When we evaluated the cytotoxicity of thesefractions, the butanol fraction showed no effects in a colonyformation assay. In addition, the butanol fraction showed acell proliferation activation effect evidenced by significantincrease in the colony formation of γ-ray irradiated cells. Both a radical scavenging activity and clonogenic activityassay suggested that the mechanism behind this protectiveeffect against reactive oxygen species may be due to theradical scavenging and cell proliferation activity of B. platyphylla var. japonica extracts.
Abstract
In our present study, total methanol extracts preparedfrom B. platyphylla var. japonica showed a significantincrease in cell proliferation upon the induction of oxidativestress by hydrogen peroxide or γ-ray irradiation. Totalmethanol extracts were fractionated into five separatepreparations i.e. n-hexane, dichloromethane, ethylacetate,n-butanol and water fractions. Among these, theethylacetate and butanol fractions of B. platyphylla var. japonica showed the highest protective effects againstoxidative stress induced by hydrogen peroxide. Thesefractions also showed strong protective effects against γ-rayirradiation. When we evaluated the cytotoxicity of thesefractions, the butanol fraction showed no effects in a colonyformation assay. In addition, the butanol fraction showed acell proliferation activation effect evidenced by significantincrease in the colony formation of γ-ray irradiated cells. Both a radical scavenging activity and clonogenic activityassay suggested that the mechanism behind this protectiveeffect against reactive oxygen species may be due to theradical scavenging and cell proliferation activity of B. platyphylla var. japonica extracts.
참고문헌 (23)
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