Buddleja officinalis prevents the normal cells from oxidative damage via antioxidant activity
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The flowers of Buddleja officinalis are used to treat sore and damaged eyes, a condition which is similar to skin wounds. However, whether it has any protective effect on oxidative DNA damage and cell death induced by hydroxyl radical remains unclear. In this study, we evaluated the protective effects of the extracts against oxidative DNA and cell damage caused by hydroxyl radical. DPPH radical, hydroxyl radical, hydrogen peroxide and intracellular ROS scavenging assay, and Fe2+ chelating assay were used to evaluate the antioxidant properties. phi X 174 RF I plasmid DNA and intracellular DNA migration assay were used to evaluate the protective effect against oxidative DNA damage. Lastly, MTT assay and lipid peroxidation assay were used to evaluate the protective effect against oxidative cell damage. It was found to prevent intracellular DNA and the normal cells from oxidative damage caused by hydroxyl radical via antioxidant activities. These results suggest that Buddleja officinalis may exert the inhibitory effect on ROS-induced carcinogenesis by blocking oxidative DNA damage and cell death.
Abstract
The flowers of Buddleja officinalis are used to treat sore and damaged eyes, a condition which is similar to skin wounds. However, whether it has any protective effect on oxidative DNA damage and cell death induced by hydroxyl radical remains unclear. In this study, we evaluated the protective effects of the extracts against oxidative DNA and cell damage caused by hydroxyl radical. DPPH radical, hydroxyl radical, hydrogen peroxide and intracellular ROS scavenging assay, and Fe2+ chelating assay were used to evaluate the antioxidant properties. phi X 174 RF I plasmid DNA and intracellular DNA migration assay were used to evaluate the protective effect against oxidative DNA damage. Lastly, MTT assay and lipid peroxidation assay were used to evaluate the protective effect against oxidative cell damage. It was found to prevent intracellular DNA and the normal cells from oxidative damage caused by hydroxyl radical via antioxidant activities. These results suggest that Buddleja officinalis may exert the inhibitory effect on ROS-induced carcinogenesis by blocking oxidative DNA damage and cell death.
참고문헌 (21)
- Armitage P., "The age distribution of cancer and amulti-stage theory of carcinogenesis", Br. J. Cancer, 8, pp.1~12 (1954)
- Barja,G., "Free radicals and aging", Trends Neurosci, 27, pp.595~600 (2004)
- Barreto,R., "Metal-induced oxidative damage in cultured hepatocytes and hepatic lysosomal fraction: beneficial effect of a curcumin", absinthiumcompound, 2005, pp.6, 31~36
- Chaudhary,A.K., "Detection of endogenous malondialdehyde-deoxyguanosine adducts in human liver", Science, 265, pp.1580~1582 (1994)
- Esterbauer,H., "Free Radicals, Lipid Peroxidation and Cancer", pp.101~128 (1982), Academic Press
- Ghosal,D., "Howradiation kills cells: survival of Deinococcus radiodurans and Shewanella oneidensis under oxidative stress", FEMS Microbiol. Rev, 29, pp.361~375 (2005)
- Grisham,M.B., "Reactive oxygenmetabolism in Reactive metabolites of oxygen and nitrogen in biology andmedicine", pp.39~ (1992), RGLanders Company
- Houghton,P.J., "Ethnopharmacology of some Buddleja species", Journal of Ethnopharmacology, 11, pp.293~308 (1984)
- Hus,B., "Antioxidant activity of hot water extract from the fruit of the Doum palm. Hyphaene thebaica", Food Chem, 98, pp.317~328 (2006)
- Jung,Y, "Oxidative DNAdamage and cytotoxicity unduced by copper-stimulated redox cycling of salsolinol, a neurotoxic tetrahydroisoquinoline alkaloid", Free Radic. Biol. Med, 30, pp.1407~1417 (2001)
- Kang,K.A., "Protective effect of irisolidone, a metabolite of kakkalide, against hydrogen peroxide induced cell damage via antioxidant effect", Bioorganic & Medicinal Chemistry, 16, pp.1133~1141 (2008)
- Leonard,S.S., "Essiac Tea: Scavenging of reactive oxygen species and effects on DNA damage", Journal of Ethnopharmacology, 103, pp.288~296 (2006)
- Livingstone, D.R., "Contaminant-stimulated reactive oxygen species production and oxidative damage in aquatic organisms", Mar. Pollut. Bull, 42, pp.656~666 (2001)
- Li,Y., "Oxidation of hydroquinone by copper: chemical mechanism and biological effects", Arch. Biochem. Biophys, 300, pp.346~355 (1993)
- Lloyd, R.V., "The origin of the hydroxyl radical oxygen in the Fenton reaction", Free Radic. Biol. and Med, 22, pp.885~888 (1997)
- Powell,C.L., "Expression of base excision DNA repair genes as a biomarker of oxidative DNA damage", Cancer Letters, 229, pp.1~11 (2005)
- Rosenkranz,A.R., "Microplate assay for the detection of oxidative products using 2',7'-dichlorofluorescindiacetate", J. Immunol. Methods, 156, pp.39~45 (1992)
- Schwarz,S.M., "On the role of superoxide anion radicals in skin tumour promotion", Carcinogenesis, 5, pp.1663~1670 (1984)
- Smirnoff,N., "Hydroxyl radical scavenging activity of compatible solutes", Phytochemistry, 28, pp.1057~1060 (1989)
- Stohs, S.J., "Oxidative mechanism in the toxicity ofmetal ions", Free Radic. Biol. and Med, 18, pp.321~336 (1995)
- Wang, H., "In vitro and in vivo antioxidant activity of aqueous extract from Choerospondias axillaris furit", Food Chemistry, 106, pp.888~895 (2008)