본문 바로가기
KERD
논문KCI 등재

Combination of Grapefruit and Rosemary Extracts Has Skin Protective Effect through MMPs, MAPKs, and the NF-κB Signaling Pathway In Vitro and In Vivo UVB-exposed Model

1

조회

0

다운로드

1

인용

0

참고문헌

키워드

초록

Long-term ultraviolet (UV) exposure accelerates the phenomenon of skin photo-aging by activating collagenase and elastase. In this study, we aimed to investigate the effects of a combination of grapefruit and rosemary extracts (cG&Re) on UVB-irradiated damage in HaCaT cells and dorsal mouse skin. In HaCaT cells, cG&Re recovered UVB-reduced cell viability and inhibited protein expression of mitogen-activated protein kinases (MAPKs), such as extracellular signal-regulated kinases (p-Erk), c-Jun N-terminal kinases (p-JNK), and a class of MAPKs (p-P38). Also, cG&Re suppressed UVB-induced collagen and elastin degradation by decreasing matrix metalloproteinases (MMPs) and nuclear factor kappa light chain enhancer of activated B cells (NF-κB) expression, which is a transcription factor. Similar results were observed in dorsal mouse skin. Taken together, our data indicate that cG&Re prevent UVB-induced skin photo-aging due to collagen/elastin degradation via activation of MAPKs, MMPs, and the NF-κB signaling pathway in vitro and in vivo.

Abstract

Long-term ultraviolet (UV) exposure accelerates the phenomenon of skin photo-aging by activating collagenase and elastase. In this study, we aimed to investigate the effects of a combination of grapefruit and rosemary extracts (cG&Re) on UVB-irradiated damage in HaCaT cells and dorsal mouse skin. In HaCaT cells, cG&Re recovered UVB-reduced cell viability and inhibited protein expression of mitogen-activated protein kinases (MAPKs), such as extracellular signal-regulated kinases (p-Erk), c-Jun N-terminal kinases (p-JNK), and a class of MAPKs (p-P38). Also, cG&Re suppressed UVB-induced collagen and elastin degradation by decreasing matrix metalloproteinases (MMPs) and nuclear factor kappa light chain enhancer of activated B cells (NF-κB) expression, which is a transcription factor. Similar results were observed in dorsal mouse skin. Taken together, our data indicate that cG&Re prevent UVB-induced skin photo-aging due to collagen/elastin degradation via activation of MAPKs, MMPs, and the NF-κB signaling pathway in vitro and in vivo.

참고문헌 (38)

  1. Afaq, F., "Botanical antioxidants in the prevention of photocarcinogenesis and photoaging", Exp. Dermatol, 15(9), pp.678~684 (2006)
  2. 백범열, "Ellagic acid plays a protective role against UV-B-induced oxidative stress by up-regulating antioxidant components in human dermal fibroblasts", The Korean Journal of Physiology & Pharmacology, 20(3), pp.269~277 (2016), 대한약리학회
  3. Black, H. S, "Reassessment of a free radical theory of cancer with emphasis on ultraviolet carcinogenesis", Integr. Cancer Ther, 3(4), pp.279~293 (2004)
  4. Bond, M., "Nuclear factor kappa B activity is essential for matrix metalloproteinase-1and-3 upregulation in rabbit dermal fibroblasts", Biochem. Biophys. Res. Commun, 264, pp.561~567 (1999)
  5. Brenneisen, P., "Ultraviolet-B irradiation and matrix metalloproteinases : from induction via signaling to initial events", Ann. N. Y. Acad. Sci, 973, pp.31~43 (2002)
  6. Chanda, S., "Microbiol. Book Series", pp.520~529 (2011)
  7. 최희정, "Inhibitory Effects of a Combination of Grapefruit and Rosemary Extracts for Alleviating UV-Induced Skin Ageing", 한국자원식물학회지, 32(3), pp.207~219 (2019), 한국자원식물학회
  8. Chung, J. H., "Aging and photoaging", J. Am. Acad. Dermatol, 49, pp.690~697 (2003)
  9. Cuvelier, M. E., "Antioxidant Constituents in Sage(Salvia officinalis)", J. Agric. Food Chem, 42, pp.665~669 (1994)
  10. Davies, K. J, "The complex interaction of matrix metalloproteinases in the migration of cancer cells through breast tissue stroma", Int. J. Breast Cancer, pp.839094~ (2014)
  11. Doyle, G. A., "Transcriptional induction of collagenase-1 in differentiated monocyte-like (U937) cells is regulated by AP-1 and an upstream C", EBP- β site, 1997, pp.272(18), 11840~11849
  12. Ficher, G.J, "Molecular basis of suninduced premature skin ageing and retinoid antagonism", Nature, 379(6563), pp.335~339 (1996)
  13. Fujii, T., "Effects of amla extract and collagen peptide on UVB-induced photoaging in hairless mice", J. Funct. Foods, 5(1), pp.451~459 (2013)
  14. González-Vallinas, M., "Rosemary(Rosmarinus officinalis L. )Extract as a Potential Complementary Agent in Anticancer Therapy", Nutr. Cancer, 67(8), pp.1221~1229 (2015)
  15. Grace, M. H., "Onestep isolation of carnosic acid and carnosol from rosemary by centrifugal partition chromatography", J. Sep. Sci, 40(5), pp.1057~1062 (2017)
  16. 함영주, "Protective Effects of Rosemary and Citrus Mixture Extracts against Photocarcinogenesis in SKH-1 Mice Model", 대한미용학회지, 11(4), pp.357~363 (2015), 대한미용학회[DOI]
  17. Jin, G. H., "UVB induced oxidative stress in human keratinocytes and protective effect of antioxidant agents", Radiat. Environ. Biophys, 46(1), pp.61~68 (2007)
  18. 전윤경, "미세캡슐화한 녹차 및 자몽종자 추출물이 Murine RAW 264.7 대식세포주의 항염증에 미치는 영향 비교", Journal of Nutrition and Health, 45(5), pp.443~451 (2012), 한국영양학회[DOI]
  19. Karin, M, "The regulation of AP-1 activity by mitogenactivated protein kinases", J. Biol. Chem, 270(28), pp.16483~16486 (1995)
  20. Khan, I. A., "Leung's encyclopedia of common natural ingredients: used in food, drugs and cosmetics", pp.1~845 (2011), John Wiley & Sons
  21. Kim, M., "Youngiasides A and C Isolated from Youngia denticulatum inhibit UVB‐induced MMP expression and promote type I procollagen production via repression of MAPK", AP‐1, Nrf 2 in HaCaT cells and human dermal fibroblasts, pp.J. Agric. Food Chem, 2015[DOI]
  22. Moore, J., "Anticancer effects of rosemary(Rosmarinus officinalis L. )extract and rosemary extract polyphenols", Nutrients, 8(11), pp.E731~ (2016)
  23. Naylor, E. C., "Molecular aspects of skin ageing", Maturitas, 69(3), pp.249~256 (2011)
  24. Park, J. E., "The protective effect of Kaempferia parviflora extract on UVB-induced skin photoaging in hairless mice", Photodermatol Photoimmunol Photomed, 30(5), pp.237~245 (2014)
  25. 박헌국, "자몽 종자 추출물의 항균성", 한국식품영양학회지, 19(4), pp.526~531 (2006), 한국식품영양학회
  26. Petiwala, S. M., "Polyphenols from the Mediterranean herb rosemary(Rosmarinus officinalis)for prostate cancer", Front. Pharmacol, 4, pp.29~ (2013)
  27. Pittayapruek, P., "Role of matrix metalloproteinases in photoaging and photocarcinogenesis", Int. J. Mol. Sci, 17(6), pp.E868~ (2016)[DOI]
  28. Quan, T., "Matrix-degrading metalloproteinases in photoaging", J. Investig. Dermatol. Symp. Proc, 14(1), pp.20~24 (2009)
  29. Rabe, J. H., "Photoaging : mechanisms and repair", J. Am. Acad. Dermatol, 55(1), pp.1~19 (2006)
  30. Rittie, L., "UV-light-induced signal cascades and skin aging", Ageing Res. Rev, 1(4), pp.705~720 (2002)
  31. Rosenthal, F. S., "The effect of prescription eyewear on ocular exposure to ultraviolet radiation", Am. j. public health, 76(10), pp.1216~1220 (1986)
  32. Sun, Z. W., "Effects of Galla chinensis extracts on UVB-irradiated MMP-1 production in hairless mice", J. Nat. Med, 69(1), pp.22~34 (2015)
  33. Vanamala, J., "Variation in the content of bioactive flavonoids in different brands of orange and grapefruit juices", J. Food Compost. Anal, 19(2-3), pp.157~166 (2006)[DOI]
  34. Wolfle, U., "UVB-induced DNA damage, generation of reactive oxygen species, and inflammation are effectively attenuated by the flavonoid luteolin in vitro and in vivo", Free Radic. Biol. Med, 50(9), pp.1081~1093 (2011)
  35. Xiaojuan, L., "Degradation pathway of carnosic acid in methanol solution through isolation and structural identification of its degradation products", Eur. Food Res. Technol, 237(4), pp.617~626 (2013)
  36. Yoshizumi, M, "Release of cytokines", chemokines and cell death in UVB-irradiated human keratinocytes, HaCaT, 2008, pp.32(11), 1405~1411
  37. Zhang, Y., "Degradation study of carnosic acid, carnosol, rosmarinic acid, and rosemary extract(Rosmarinus officinalis L. )assessed using HPLC", J. Agric. Food Chem, 60(36), pp.9305~9314 (2012)
  38. Zhou, X., "Synergistic effects of Chinese herbal medicine : a comprehensive review of methodology and current research", Front. Pharmacol, 7, pp.201~ (2016)

관련 논문AI추천

Inhibitory Effects of a Combination of Grapefruit and Rosemary Extracts for Alleviating UV-Induced Skin Ageing김태호, 이상한, 차영호, 최희정, Md. Badrul Alam, Peijun Zhao한국자원식물학회 · 한국자원식물학회지2019 · 제32권 제3호Actinidia polygama Water Extract (APWE) Protects Against UVB-Induced Photoaging via MAPK/AP-1 and TGFβ-Smad Pathway김다영, 김범준, 김수영, 김유진, 박아연, 유광호, 이정민, 이정옥, 장유나, 호승현, Su-Jin Park대한피부과학회 · Annals of Dermatology2024 · 제36권 제1호Protective effects of red orange (Citrus sinensis [L.] Osbeck [Rutaceae]) extract against UVA-B radiation-induced photoaging in Skh:HR-2 miceJung Jae In, Kim Eun Ji, Kim Yoon Hee, Lim Cho Young, Tae Young Kim한국영양학회 · Nutrition Research and Practice2023 · 제17권 제4호으름 열매 추출물의 MAPKs, NF-κB 신호전달경로를 통한 MMP-1 억제 및 항노화 효과권순현, 김용민, 하헌용대한미용학회 · 대한미용학회지2024 · 제20권 제4호Protective Effects of Standardized Siegesbeckia glabrescens Extract and Its Active Compound Kirenol against UVB-Induced Photoaging through Inhibition of MAPK/NF-κB Pathways김미보, 김종욱, 윤준곤, 황재관한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2017 · 제27권 제2호Topical or oral treatment of peach flower extract attenuates UV-induced epidermal thickening, matrix metalloproteinase-13 expression and pro-inflammatory cytokine production in hairless mice skin곽충실, 신창엽, 양지원, 정진호한국영양학회 · Nutrition Research and Practice2018 · 제12권 제1호

이 간행물의 다른 논문 (13건)