순화된 인삼 조직배양묘의 현장 재배시 적응성 및 진세노사이드 조성 안정성
Adaptability and Ginsenoside Compositional Stability of Acclimatized Panax ginseng Plantlets Derived from Somatic Embryogenesis under Field Conditions
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Background: Panax ginseng C. A. Meyer is a major medicinal crop in Korea; however, its low seed propagation efficiency limits the distribution of new cultivars. Somatic embryogenesis has been developed as an alternative propagation method. Nevertheless, the adaptability and compositional sta- bility of acclimatized in vitro-grown roots (IGRs) under field conditions remain unclear. Methods and Results: IGRs of the ‘Cheonryang’ cultivar were produced by somatic embryogenesis, acclimatized in a greenhouse, and categorized into three weight groups (< 0.8 g, 0.8 – 1.4 g, > 1.4 g). They were transplanted into an experimental field, with seed-derived seedlings serving as controls. The emergence rate increased with IGR weight, reaching 88.0% in the > 1.4 g group, which was com- parable to the control (86.6%). Multi-stem formation occurred frequently in IGRs, reaching 46.2% in the > 1.4 g group. Flowering was observed in IGRs ≥ 0.8 g but not in controls. Although aerial traits were smaller in IGRs, growth improved with increasing initial root weight. IGRs > 1.4 g were similar in weight and length to the controls but exhibited larger diameters and more rhizomes. Ginsenoside composition and total content did not differ significantly between groups. Conclusions: These findings confirm the field adaptability and compositional equivalence of acclimatized IGRs, supporting their practical use in large-scale propagation.
Abstract
Background: Panax ginseng C. A. Meyer is a major medicinal crop in Korea; however, its low seed propagation efficiency limits the distribution of new cultivars. Somatic embryogenesis has been developed as an alternative propagation method. Nevertheless, the adaptability and compositional sta- bility of acclimatized in vitro-grown roots (IGRs) under field conditions remain unclear. Methods and Results: IGRs of the ‘Cheonryang’ cultivar were produced by somatic embryogenesis, acclimatized in a greenhouse, and categorized into three weight groups (< 0.8 g, 0.8 – 1.4 g, > 1.4 g). They were transplanted into an experimental field, with seed-derived seedlings serving as controls. The emergence rate increased with IGR weight, reaching 88.0% in the > 1.4 g group, which was com- parable to the control (86.6%). Multi-stem formation occurred frequently in IGRs, reaching 46.2% in the > 1.4 g group. Flowering was observed in IGRs ≥ 0.8 g but not in controls. Although aerial traits were smaller in IGRs, growth improved with increasing initial root weight. IGRs > 1.4 g were similar in weight and length to the controls but exhibited larger diameters and more rhizomes. Ginsenoside composition and total content did not differ significantly between groups. Conclusions: These findings confirm the field adaptability and compositional equivalence of acclimatized IGRs, supporting their practical use in large-scale propagation.