인삼 염류내성 계통의 선발을 위한 배배양 배지조건

Abstract

Korean ginseng(Panax ginseng .A. Meyer) is very difficult to obtain stable production of qualified ginseng roots because of variable stresses in soil environments. In environment stresses, soil condition is the most important factor, among which nutrients, especially inorganic materials such as N, P, K, Ca, Mg, Fe, etc., influence greatly on the ginseng growth. However, present ginseng field soils in Korea contain so much amount of such inorganic materials that a variety of remarkable disorders were noted in many ginseng plantations, resulting in decrease of qualitative ginseng production. Therefore, it is required to search for genetic resources and genes tolerant to salt stress for the development of ginseng cultivars. Selection of stress-tolerant ginseng lines in fields is very difficult because it is almost impossible to control properly the environmental conditions of soil. On the contrary, it can be studied with ease to search for stress-tolerant ginseng lines through in vitro culture because of easy manipulation of stress conditions. Murashige＆ Skoog(MS) media with 2.5 folds of KNO₃, NH₄NO₃, MgSO₄. 7H₂O, KH₂PO₄, and CaCl₂.2H₂O was established for the selection of ginseng lines tolerant to salt stress under the embryo culture.