방사선조사 유래의 조기출수 식물(Oryza sativa L.) 계통의 유전적 변이 분석
Genetic Variation Analysis of Early-heading Plant (Oryza sativa L.) Lines Derived from Gamma-ray Irradiation
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참고문헌
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초록
식물유전자원의 소재를 확대하고자 동진 1호 벼의 건조종자에 감마선(60Co, 300 Gy)을 조사하여 선발한 조기출수 계통의 유전적 변이성을 분석하였다. 선발한 조기출수계통의 M7(2010년)과 M8(2011년) 세대에서 평균 출수기는대조품종인 동진 1호(중만생종) 보다 각각 11일(γ-2 계통),10일(γ-5 계통), 6일(γ-1 계통), 5일(γ-3 계통), 4일(γ-4계통)이 빠르게 나타났다. ISSR 분석 결과, 선발계통의 유전적 다형성은 γ-2 계통 5.9%, γ-1 계통 7.5%, γ-4 계통15.1%, γ-3 계통 15.3%, X-1 계통 19.4%, γ-5 계통23.4%로 대조품종의 4.3% 보다 높게 나타나 방사선 조사로 DNA 수준에서 변이가 증가되었음을 확인하였다. 엽록체 DNA(rps16-trnK 영역) 분석 결과, 염기 길이는 대조품종과 같은 664 bp인 반면, 총 5개 염기서열 영역에서 치환 변이가 발생하였고 그 중 6 bp 및 587 bp 영역의 변이는 선발 계통에서만 나타났다.
Abstract
This study was carried out to evaluate genetic variation of early-heading rice (Oryza sativa L. cv. Dongjin 1) lines derived from gamma-ray (60Co, 300 Gy) irradiation. The average heading date of the 5 early-heading lines in M7 and M8generation was faster than that of untreated control as 11 (line γ-2), 10 (line γ-5), 6 (γ-1 line), 5 (γ-3) and 4 days (line γ-4),respectively. According to ISSR analysis, polymorphic rate of the early-heading lines (from 5.9% to 23.4%) was higher than that of control (4.3%). The result indicates that the gamma-ray promote variation at DNA level. When genetic variations of rps16-trnK region were evaluated by nucleotide analysis, nucleotide length of the rps16-trnK region was 664 bp in all the early-heading lines and control. Out of 5 sites of nucleotide transposition detected in the region, however, 2 sites were appeared only in the early-heading lines.
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