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감마선 조사에 따른 수수 [Sorghum bicolor (L.) Moench]의감수성 평가 및 우수농업형질 변이체 선발

Evaluation of Radiosensitivity to Gamma-ray Irradiation and Selection of Lines with Elite Agricultural Traits in Sorghum [Sorghum bicolor (L.) Moench]

이민규|홍민정|김진백|김동건|류재혁|권순재|하보근|강시용|안준우|김정민
(사)한국방사선산업학회 (Korean Society of Radiation Industry)|2018.03|방사선산업학회지 (Journal of Radiation Industry)|제12권 제1호|pp. 65-74|DOI 10.23042/radin.2018.12.1.65

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키워드

Gamma-raySorghumOptimal doseMedian lethal dose(LD50)Median reduction dose(RD50)

초록

This study was carried out to examine radio-sensitivity to gamma-rays irradiated at different doses and to determine optimal dose for mutation breeding in sorghum [Sorghum bicolor(L.) Moench]. Ten cultivars were irradiated with gamma-rays at 100, 200, 300, 400, 500, 600, 700, and 1000 Gy. The germination rate of M1 seeds was gradually decreased at doses higher than 300 Gy and showed no germination over 600 Gy. Likewise, the survival rate of M1 seedling decreased at doses higher than 200 Gy and there were no survival over 500 Gy in all cultivars. However, the median lethal doses(LD50) and the median reduction doses(RD50) values of the tested sorghum cultivars were slightly different between cultivars. The LD50 was 240(DINE-A-M), 225(IS225), 335(IS2868), 180(Moktak), 214(Banwoldang), 213(ChalI), 231(ChalII), 182(KLSO79125), 287(KLSO79075), and 274(Mesusu) Gy. The RD50 was 179(DINEA- M), 175(IS225), 210(IS2868), 142(Moktak), 174(Banwoldang), 163(ChalI), 175(ChalII), 159(KLSO79125), 199(KLSO79075), and 195(Mesusu) Gy depending on the plant height. Afterwards, we confirmed growth characteristics and mutants of the M2 generation and then were collected mutant lines better than original cultivars. As a result, we suggest that the optimal doses of gamma-ray irradiations for mutation breeding of sorghum are 180 Gy(Moktak, KLSO79125),200 Gy(DINE-A, IS645, Banwoldang, ChalI, ChalⅡ), 250 Gy(KLSO79075, Mesusu), and 300 Gy(IS2868). By irradiation of gamma-rays at the suggested optimal doses, we constructed M2 lines for sorghum breeding.

Abstract

This study was carried out to examine radio-sensitivity to gamma-rays irradiated at different doses and to determine optimal dose for mutation breeding in sorghum [Sorghum bicolor(L.) Moench]. Ten cultivars were irradiated with gamma-rays at 100, 200, 300, 400, 500, 600, 700, and 1000 Gy. The germination rate of M1 seeds was gradually decreased at doses higher than 300 Gy and showed no germination over 600 Gy. Likewise, the survival rate of M1 seedling decreased at doses higher than 200 Gy and there were no survival over 500 Gy in all cultivars. However, the median lethal doses(LD50) and the median reduction doses(RD50) values of the tested sorghum cultivars were slightly different between cultivars. The LD50 was 240(DINE-A-M), 225(IS225), 335(IS2868), 180(Moktak), 214(Banwoldang), 213(ChalI), 231(ChalII), 182(KLSO79125), 287(KLSO79075), and 274(Mesusu) Gy. The RD50 was 179(DINEA- M), 175(IS225), 210(IS2868), 142(Moktak), 174(Banwoldang), 163(ChalI), 175(ChalII), 159(KLSO79125), 199(KLSO79075), and 195(Mesusu) Gy depending on the plant height. Afterwards, we confirmed growth characteristics and mutants of the M2 generation and then were collected mutant lines better than original cultivars. As a result, we suggest that the optimal doses of gamma-ray irradiations for mutation breeding of sorghum are 180 Gy(Moktak, KLSO79125),200 Gy(DINE-A, IS645, Banwoldang, ChalI, ChalⅡ), 250 Gy(KLSO79075, Mesusu), and 300 Gy(IS2868). By irradiation of gamma-rays at the suggested optimal doses, we constructed M2 lines for sorghum breeding.

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