우엉뿌리추출물이 ICAM-1과 NO조절에 미치는 항염증효과
Anti-inflammatory Effects by Arctium lappa L. Root Extracts through the Regulation of ICAM-1 and Nitric Oxide
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초록
본 연구는 우엉추출물의 항염증효과를 측정하였다. 인간유래 폐상피세포 A549를 이용하여 염증반응을 유발하고유지시키는데 중요한 역할을 나타낸다고 알려진 ICAM-1의 억제적 조절 효과를 평가하였으며, 염증매개인자로 알려진 NO와 이의 생성에 영향을 미치는 iNOS의 발현조절에서도 억제효과가 있음을 확인하였다. 또한, 우엉추출물이 인간각질형성세포주의 세포증식에 효과가 있음을 나타내었다. 본 연구에서 사용되는 폐상피세포는 호흡기질환의천식 등의 알러지 및 만성염증연구에 주로 활용되고 있으며, 각질형성세포는 아토피 피부염의 만성 염증반응에서주요하게 확인하는 요소이다. 따라서 본 연구결과에서 나타난 우엉추출물의 항염증 효과는 다양한 염증반응에서도특히, 알러지 및 아토피 피부염 질환을 더욱 악화시키는 만성 염증에 있어서 우엉추출물이 유용한 소재로 사용될 수있음을 시사한다고 할 수 있다.
Abstract
Atopic dermatitis is a chronic, allergic inflammatory skin disease that is accompanied by pruritic chronic eczema and markedly increased levels of inflammatory cells in endothelial cells. Arctium lappa L. is a popular edible vegetable cultivated in Asia. This study examined the effect of butanol extracts of A. lappa (ALBE) on the expression of adhesion molecule, ICAM-1 and the production of NO-iNOS induced by TNF-alpha in A549 endothelial cells. We also studied the effects of ALBE on the proliferation of keratinocyte. We observed significant inhibition of NO-iNOS production in dose-dependant manners and ALBE at 100 μg/mL suppressed the expression of ICAM-1 in TNF-α-induced A549 cells. In addition, the treatment of ALBE for 48 hrs increased the proliferation of HaCaT keratinocytes. These results support that ALBE has an anti-inflammatory effects for the treatment of atopic dermatitis.
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