Genetic Diversity and Relationship Analysis of Genus Taraxacum Accessions Collected in Korea
2
조회
0
다운로드
3
인용
0
참고문헌
키워드
초록
Genus Taraxacum has been widely used as a folkloric medicine for treatment of diverse diseases. The genetic diversity and relationship among 32 accessions belonging to five Taraxacum species (T. mongolicum T. coreanum, T. coreanum var. flavescens, T. officinale and T. laevigatum) which collected from field, mountain, island and seaside of Korea were evaluated using ISSR markers. A total of 142 ISSR loci detected in the overall species were all polymorphic loci (100%) and interspecies polymorphisms obtained from Korean native and naturalized species were 98.2% and 94.5%,respectively. The genetic similarity matrix (GSM) among 32 accessions ranged from 0.025 to 0.860 with an average of 0.303. According to the clustering analysis, the Korean native species and naturalized species were divided two major clusters. In addition, the different species were divided into independent groups except for the T. coreanum and T. coreanum var. flavescens, and all the 32 accessions could be classified into 7 categories. The study findings indicate that Taraxacum accessions have a high genetic diversity and the dandelion accessions as breeding materials can be effectively utilized for the improvement of Taraxacum breeding.
Abstract
Genus Taraxacum has been widely used as a folkloric medicine for treatment of diverse diseases. The genetic diversity and relationship among 32 accessions belonging to five Taraxacum species (T. mongolicum T. coreanum, T. coreanum var. flavescens, T. officinale and T. laevigatum) which collected from field, mountain, island and seaside of Korea were evaluated using ISSR markers. A total of 142 ISSR loci detected in the overall species were all polymorphic loci (100%) and interspecies polymorphisms obtained from Korean native and naturalized species were 98.2% and 94.5%,respectively. The genetic similarity matrix (GSM) among 32 accessions ranged from 0.025 to 0.860 with an average of 0.303. According to the clustering analysis, the Korean native species and naturalized species were divided two major clusters. In addition, the different species were divided into independent groups except for the T. coreanum and T. coreanum var. flavescens, and all the 32 accessions could be classified into 7 categories. The study findings indicate that Taraxacum accessions have a high genetic diversity and the dandelion accessions as breeding materials can be effectively utilized for the improvement of Taraxacum breeding.
참고문헌 (33)
- Ahn, Y.H., "Analysis of genetic variance among Taraxacum officinale growing in each populated areas using RAPD", Environment and Ecol, 17(1), pp.27~31 (2003)
- Ahn, Y.H., "Analysis of genetic relationship among native Taraxacum and naturalized Taraxacum species using RAPD", Environment Ecol, 17(2), pp.169~176 (2003)
- Akashi, T., "Biosynthesis of triterpenoids in cultured cells, and regenerated and wild plant organs of Taraxacum officinale", Phytochemistry, 36, pp.303~308 (1994)
- Bartish, I.V., "RAPD analysis of interspecific relationships in presumably apomictic Cotoneaster species", Euphytica, 120, pp.273~280 (2001)
- Battjes, J., "Clonal diversity in some microspecies of Taraxacum sect. Palustria (Lindb. fil.) Dahlst. from Czechoslowakia", Botanische Jahrbücher für Systematik, Pflanzengeschichte and Pflanzengeographie, 114, pp.315~328 (1992)
- Becelaere, G.V., "Pedigree vs. DNA marker-based genetic similarity estimates in cotton", Crop Sci, 45, pp.2281~2287 (2005)
- 천상욱, "서양민들레 부위별 추출물의 항산화 및 세포독성 연구", 한국자원식물학회지, 25(2), pp.232~239 (2012), 한국자원식물학회[DOI]
- Christoph, R., "Molecular differentiation between coexisting species of Taraxacum sect. Erythrosperma (Asteraceae) from populations in south-east and West Germany", Linnean Soc, 142(1), pp.109~117 (2004)
- Esselman, E., "Clonal diversity in the rare Calamagrosis porteri ssp. insperata (Poaceae): comparative results for allozymes and random amplified polymorphic DNA (RAPD) and their simple sequence repeat (ISSR) markers", Molecul. Ecology, 8, pp.443~451 (1999)
- Fang, G., "A quick inexpensive method of removing ploysaccharides from plant genomic DNA", Biotechniques, 13, pp.52~55 (1992)
- Godwin, I.D., "Application of inter simple sequence repeat (ISSR) markers to plant genetics", Electrophoresis, 18, pp.1524~1528 (1997)
- Hulst, R.G.M van D, "Amplified fragment length polymorphism (AFLP) markers reveal that population structure of triploid dandelions (Taraxacum officinale) exhibits both clonality and recombination", Molecul. Ecology, 9, pp.1~8 (2000)
- 이정윤, "Genetic Diversity Assessment and Phylogenetic Analysis of Peanut (Arachis hypogaea L.) in RDA Genebank Collection using SSRs", 한국자원식물학회지, 24(3), pp.272~279 (2011), 한국자원식물학회
- Katrin, S., "Taraxacum-A review on its phytochemical and pharmacological profile", Ethnopharmacology, 107, pp.313~323 (2006)
- Kirschner, J., "Principal features of the cpDNA evolution in Taraxacum (Asteraceae, Lactuceae): a conflict with taxonomy", Plant Syst. Evol, 239, pp.231~255 (2003)
- Kitamura, S., "Compositae Japonicae", Mem. Coll. Sci. Univ. Kyoto. Japan, 24, pp.1~42 (1957)
- Kwon, Y.C., "RAPD analysis in Korean native and introduced Chinese chives (Allium spp.)", Korean J. Breed, 30, pp.298~304 (1998)
- Lee, T.B., "Illustrated flora of Korea", pp.783~784 (1993), Hyangmoonsa press
- 이현화, "흰민들레와 서양민들레 추출물의 세포독성 및 항산화 활성 비교", 한국약용작물학회지, 16(2), pp.79~85 (2008), 한국약용작물학회
- Lyu, J.H., "Genetic diversity among tea (Camellia sinensis) accessions based on random amplified polymorphic DNA (RAPD) patterns", Plant Res, 6(3), pp.195~204 (2003)
- Menz, M.A., "Genetic diversity of public inbreds of sorghum determined by mapped AFLP and SSR markers", Crop Sci, 44, pp.1236~1244 (2004)
- Morita, T., "Geographical distribution of diploid and polyploid Taraxacum in Japan", Bull. Natn. Sci. Mus, 2, pp.23~38 (1976)
- Nakai, T., "A synoptical sketch of Korean flora", Bul. Nat. Sci. Mus, 31, pp.129~135 (1952)
- Richards, A.J., "The origin of Taraxacum agamospecies", Bot. J. Linn. Soc, 66, pp.189~211 (1973)
- Russel, TR, "Direct comparison of level of genetic variation among barley accessions detected by RFLPs, AFLPs, SSRs and RAPDs", Theor. Appl. Genet, 95, pp.714~722 (1997)
- Ryu, J.H., "Growth and development characteristics and genetic diversity analysis of genus Taraxacum accessions collected in Korea", 박사, pp.2012
- 류재혁, "ISSR 표지에 의한 서양민들레와 흰민들레 수집종의 유전적 다양성 및 유연관계 분석", 한국약용작물학회지, 19(3), pp.149~156 (2011), 한국약용작물학회
- 류재혁, "방사선조사 유래의 조기출수 식물(Oryza sativa L.) 계통의 유전적 변이 분석", 한국자원식물학회지, 25(1), pp.142~151 (2012), 한국자원식물학회
- Sneath, P.H.A., "Numerical Taxonomy. 1st Edn.", pp.573~ (1973), W.H. Freeman and Co.
- Sica, M, "ISSR markers show differentiation among Italian populations of Asparagus acutifolius L", Genetics, 6(17), pp.1~7 (2005)
- "The Korea Herbal Pharmacopoeia", Korea Food & Drug Administration Announcement no. 2011-26, 376~ (2011)
- Wu, Z., "Genetic diversity of Taraxacum germplasm revealed by sequence-related amplified polymorphism (SRAP) analysis", African J. Biotechnol, 10(47), pp.9557~9562 (2011)
- Zietkiewicz, E., "Genome fingerprinting by SSR anchored PCR amplification", Genomics, 20, pp.176~183 (1994)