Evaluation of Genetic Diversity among Korean Wheat Using RAPD and ISSR Analysis
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
The genetic diversity was evaluated using RAPD and ISSR among natural populations and Korean wheat cultivars (Triticum aestivum). Understanding the genetic diversity of putative parental and wild stocks would be useful in wheat breeding programs. Ninety three populations were evaluated with fifty RAPD and three ISSR primers. A total of 185 RAPD and ISSR polymorphism were produced. These markers were considered to estimate the genetic distance among accessions. The genetic similarity ranged from 0.41 to 0.86. The dendrogram were constructed by using the UPGMA clustering algorithm based on genetic similarity. The genetic diversity within and among accession was assessed through Principal Component Analysis (PCA) for statistics analysis. In cluster analysis, four groups were clustered and 17 accessions were not clustered. The PCA was corresponded well to the result. This study provides basic information about the genetic relationships for breeding purposes.
Abstract
The genetic diversity was evaluated using RAPD and ISSR among natural populations and Korean wheat cultivars (Triticum aestivum). Understanding the genetic diversity of putative parental and wild stocks would be useful in wheat breeding programs. Ninety three populations were evaluated with fifty RAPD and three ISSR primers. A total of 185 RAPD and ISSR polymorphism were produced. These markers were considered to estimate the genetic distance among accessions. The genetic similarity ranged from 0.41 to 0.86. The dendrogram were constructed by using the UPGMA clustering algorithm based on genetic similarity. The genetic diversity within and among accession was assessed through Principal Component Analysis (PCA) for statistics analysis. In cluster analysis, four groups were clustered and 17 accessions were not clustered. The PCA was corresponded well to the result. This study provides basic information about the genetic relationships for breeding purposes.
참고문헌 (18)
- Amiraju, JS, "Identification of inter-simplesequence repeat (ISR) markers associated with seed sizein wheat", 102, pp.726~732 (2001)
- Barrett BA, "AFLP based geneticdiversity assessment among wheat cultivars from thePacific Northwest", 38, pp.1261~1271 (1998)
- Chen HB, "Genetic diversity in hard red spring wheat based onmolecular markers", pp.1628~1632 (1994)
- Devos KM, "The use of randomamplified polymorphic DNA markers in wheat", pp.567~572 (1992)
- Ha YW, "Protein of majorgermplasms for high-protein wheat breeding", pp.393~402 (1990)
- He S, "Detection of DNAsequence polymorphisms among wheat cultivars Evaluation of Genetic Diversity among Korean Wheat Using RAPD and ISR Ana lysis 315", pp.573~578 (1992)
- Joshi CP, "Application of the randomamplified polymorphic DNA technique for detection ofpolymorphism among wild and cultivated tetraploidwheats", ge(me 36), pp.602~609 (1993)
- Kojima T, "Geneticlinkage map of ISSR and RAPD markers in Einkorn wheatin relation to that of RFLP markers", pp.37~45
- Le CK, "Comparison of Physico-chemaical properties betwenwaxy and non-waxy wheat grains 1998 Diversity evaluation of wheat germplasm resourcesin China", pp.2 419 167~427 172
- Mari S, "Genetic diversity of hexaploid wheat cultivarsestimated by RAPD markers morphological traits andcoefficients of parentage", pp.366~369
- Nagaoka T, "Aplicability of inter-simplesequence repeat polymorphisms in wheat for use asDNA markers in comparison to RFLP and RAPD markers", 94, pp.597~602 (1997)
- Olufowote JO, "Comparativeevaluation of within-cultivar variation or ice (Oryza sativaL.) using microsatellite and RFLP markers", 40, pp.370~378 (1997)
- Park CS, "Characteristicsof biochemical markers and whole-wheat flours usingsmall-scaled sampling methods in Korean wheats", 50, pp.346~35 (2005)
- Rohlf FJ, "NTSYS-pc Numerical taxonomy andmultivariate analysis system version 2.1 manual" (2000), ApliedBiostatistics, NewYork. USA.
- Sneath PHA, "Theprinciples and practice of numerical classification", numerical taxo(my) (1973)
- Michener CD, "University of KansasScientific Bulletin 38", pp.1409~1438 (1958)
- Vierling RA, "Use of RAPD markersto determine the genetic diversity of diploid wheatgenotypes", pp.835~838 (1992)
- Vierling RA, "using PCR with arbitrary primers", pp.7213~7218 (1992)