갈대(Phragmites communis Trinius) 성숙종자를 이용한 기내 식물체 재분화와 재분화체의 유전적 다양성
Plant Regeneration and Genetic Diversity of Regenerants from Seed-derived Callus of Reed (Phragmites communis Trinius)
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활용가치가 높은 부존식물자원인 갈대의 기내 번식을 통한 배양체계를 확립하고 재분화 식물체들의 유전적 다양성을 검토한 결과, 성숙종자 유래의 캘러스를 통한 기내 식물체 재분화는 N6배지에서 MS배지보다 양호하였고, 0.25~0.5 mg/L의 BA를 포함한 N6배지에서 가장 높았다. ISSR 마커를 이용하여 재분화 식물체의 유전적 안정성을 분석한결과, 검출된 총 94 유전좌중 유전적 다형성은 17%였고, 평균 유전자다양도 값(h)은 0.03, BA 5 mg/L를 포함한 N6배지에서 0.008, NAA 0.1 mg/L와 kinetin 2 mg/L를 포함한MS 배지에서 0.040으로 나타났다. 이것은 재분화된 갈대식물체 개체간에 유전적으로 구조가 매우 단순하고 균일하며,유전적 다양성 진단에 ISSR 마커가 효과적임을 시사한다.
Abstract
This study was carried out to address an efficient in vitro regeneration system from seed-derived callus ofPhragmites communis, and to evaluate genetic variations of the regenerants using ISSR markers. Shoot regeneration viacalli was greatly influenced by N6 medium compared with MS medium, and plant regeneration frequency was 90% in N6supplemented with BA 0.25 mg/L and BA 0.5 mg/L. According to ISSR analysis of the thirty regenerants, out of 94 locidetected overall, 16 were identified to be polymorphic with a rate (PR) of 17.0%. The mean gene diversity (h) of differentin vitro condition was 0.03 and ranged from 0.008 for N6 with BA 5 mg/L, to 0.040 for MS with IAA 0.1 mg/L+kinetin 2mg/L. The results indicate that the regenerants have a low genetic variation, and ISSR analysis is effective to detect geneticvariation of regenerants.
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