비쭈기 나무(Cleyera japonica) 추출물의 생리활성에 대한 연구
Study on the Physiological Activities of Cleyera japonica Extract
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초록
적 요본 연구에서 비쭈기 나무의 잎 부분에 대한 다양한 생리활성을 조사하여 기능성소재 응용가능성을 검토하였다. 비쭈기 나무 잎 추출물은 멜라노마 세포에 대하여 낮은 세포독성을 나타냈다. 비쭈기 나무 추출물 처리 시 항산화(IC50, 22.90 ㎎/L) 및항균활성이 매우 우수하게 나타났으며 특히 그람양성세균에 대한 항균활성이 높았다. 또한, 비쭈기 나무 추출물 처리시tyrosinase 활성저해 및 멜라닌 함량 저하(IC50, 101.90 ㎎/L)를보여주었다. 이와 같은 결과로 미루어 볼 때 비쭈기 나무 추출물은 피부미백 소재 등 피부개선 효과를 비롯한 기능성 화장품에활용하기 위한 매우 효과적인 재료가 될 수 있다고 판단된다
Abstract
In this study, we investigated the applicability of functional materials by examining a variety of physiological activities with the extract of Cleyera japonica leaf. Cleyera japonica extract showed a low cytotoxicity against murine melanoma B16F10 cells. In little or no cytotoxicity at concentrations, we showed that the treatment with Cleyera japonica extract resulted in a significant increase in the DPPH radical scavenging activity (IC50, 22.90 ㎎/L), similar to ascorbic acid (IC50, 18.65 ㎎/L) and anti-microbial activities against Bacillus subtilis, Escherichia coli, and Candida albicans. In particular, anti-microbial activities against Gram-positive bacteria was high. These results suggest that Cleyera japonica extract could be used as a natural preservative. Additionally, Cleyera japonica extract showed the inhibition of tyrosinase activity (IC50, 178.90 ㎎/L), similar to kojic acid (IC50, 89.13 ㎎/L) and decreased melanin content (IC50, 101.90 ㎎/L) higher than the control arbutin level (IC50, 100.65 ㎎/L), especially. Therefore, these results indicate that Cleyera japonica extract may be an effective material for functional cosmetics such as skin whitening materials.
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