누리장나무 추출물의 세포독성 및 항산화 활성효과 연구
A Study on Cytotoxicity and Antioxidant Activity of Clerodendrum Trichotomum Extract
1
조회
0
다운로드
3
인용
0
참고문헌
키워드
초록
In this study, an antioxidant activity, anti-inflammatory and antibacterial experiment was conducted to develop natural cosmetic materials depending on biological activity and cytotoxicity of the Clerodendrum trichotomum extract and the results were as follows. The content of polyphenol was 119.723±1.0㎎/g and when different concentrations of the extract were applied, DPPH radical scavenging activity was measured at 3.2, 4.0, 13.5, 24.7, 77.1, and 78.1% respectively. This means that the higher the concentration, the higher antioxidant activity. For antibacterial measurement, S. aureus, S. epidermidis, E. coli, and P. acnes were added. As a result, S. aureus and P. acnes had the highest value(16㎜) at a concentration of 1㎎/㎖, S. aureus had the highest value(16㎜) at a concentration of 5㎎/㎖, and S. epidermidis had the highest value(15㎜) at a concentration of 10㎎/㎖. Also, S. aureus and S. epidermidis had the highest value(16㎜) at a concentration of 20㎎/㎖. ABTS radical scavenging activity was measured at 0.645, 4.677, 15.054, 31.774, 101.237, and 101.022% respectively, when different concentrations of the extract were applied. The result was greater than or similar to Vit and C, which are standard controls, at a concentration of 500 and 1000㎍/㎖. The cell survival rate was 92.738, 84.260, 83.964, 86.987, 86.589, and 70.497% and there was no cytotoxicity of the extract. The expression of iNOS declined when th concentration of the extract was 500㎍/㎖ and in the COX-2 experiment, RAW 264.7 using the pre-treated extract failed to inhibit COX-2 that increased due to LPS. In conclusion, it is considered that the findings of this study can be used as important basic data to develop natural cosmetic materials using the Clerodendrum trichotomum extract.
Abstract
In this study, an antioxidant activity, anti-inflammatory and antibacterial experiment was conducted to develop natural cosmetic materials depending on biological activity and cytotoxicity of the Clerodendrum trichotomum extract and the results were as follows. The content of polyphenol was 119.723±1.0㎎/g and when different concentrations of the extract were applied, DPPH radical scavenging activity was measured at 3.2, 4.0, 13.5, 24.7, 77.1, and 78.1% respectively. This means that the higher the concentration, the higher antioxidant activity. For antibacterial measurement, S. aureus, S. epidermidis, E. coli, and P. acnes were added. As a result, S. aureus and P. acnes had the highest value(16㎜) at a concentration of 1㎎/㎖, S. aureus had the highest value(16㎜) at a concentration of 5㎎/㎖, and S. epidermidis had the highest value(15㎜) at a concentration of 10㎎/㎖. Also, S. aureus and S. epidermidis had the highest value(16㎜) at a concentration of 20㎎/㎖. ABTS radical scavenging activity was measured at 0.645, 4.677, 15.054, 31.774, 101.237, and 101.022% respectively, when different concentrations of the extract were applied. The result was greater than or similar to Vit and C, which are standard controls, at a concentration of 500 and 1000㎍/㎖. The cell survival rate was 92.738, 84.260, 83.964, 86.987, 86.589, and 70.497% and there was no cytotoxicity of the extract. The expression of iNOS declined when th concentration of the extract was 500㎍/㎖ and in the COX-2 experiment, RAW 264.7 using the pre-treated extract failed to inhibit COX-2 that increased due to LPS. In conclusion, it is considered that the findings of this study can be used as important basic data to develop natural cosmetic materials using the Clerodendrum trichotomum extract.
참고문헌 (30)
- Halliwell B., "DNA damage by oxygen-derived species", FEBS Letters, 281, pp.9~19 (1991)
- 고경숙, "황금추출물의 항산화 활성 및 세포독성 연구", 14(3), pp.223~243 (2013), 한국인체미용예술학회
- H. N. Jnawali, "Antiinflammatory activity of rhamnetin and a model of its binding to c-Jun NH2-terminal kinase 1 and p38 MAPK", J Nat Prod, 77, pp.258~263 (2014)
- 김대용, "피부미용소재로서 보리순 추출물의 생리활성 특성 연구", 한국인체미용예술학회지, 17(4), pp.143~155 (2016), 한국인체미용예술학회
- 김미경, "블루베리 잎 추출물의 항산화 및 항염증 효과", 한국인체미용예술학회지, 15(4), pp.109~120 (2014), 한국인체미용예술학회
- 김미연, "백작약 에탄올 추출물의 화장품 약리활성 검증", 한국미용학회지, 22(4), pp.818~823 (2016), 한국미용학회
- 오수연, "누리장나무(Clerodendrontrichotomum) 잎 추출물의 항염증 효과 연구", 과학교육연구논총, 30(1), pp.1229~1238 (2014)
- 추한나, "누리장나무차가 항고혈압에 미치는 영향", 대한본초학회지, 30(4), pp.129~135 (2015), 대한본초학회
- Kawai, K., "Clerodendrin I, a New Neoclerodane Diterpenoid from Clerodendron trichotomum", Biosci. Biotechnol. Biochem, 63, pp.1795~1797 (1999)
- Sigleton V. L., "Colorimetry of total phenolics with phosphomolybdicphosphotungstic acid reagents", American of Journal Enology and Viticulture, 16(3), pp.144~158 (1965)
- Blois MS, "Antioxidant determination by the use of a stable free radical", Nature, 181, pp.1199~1200 (1958)
- Higashi G. S., "Appraisement of antioxidative activity from vegetables", Japan Journal of Food Industry, 57(1), pp.56~64 (2000)
- Pellegrin, N., "Screening of diatry carotenoids and carotenoid-rich fruit extracts for antioxidant activities applying 2,2'-azinobis (3-ethylbenzo-thiazoline-6-sulfonic acid) radical cation decolorization assay", Method Enzymol, 299, pp.379~389 (1998)
- C. A. Begona, "Fermented wheat germ extract inhibits glycolysis pentose cycle enzymes and induces apoptosis through poly (ADP-ribose) polymerase activation in Jurkat T cell leukemia tumor cells", The Journal of Biological Chimistry, 277(48), pp.46408~46414 (2002)
- Yoshizawa S, "AntitμMor promoting activity of epigallocatechin gallate, the main constitutent of tannin in green tea", Phytother Res, 1, pp.44~47 (1987)
- 왕명현, "화살나무 잎 열수추출물의 생리활성", 한국식품과학회지, 39(5), pp.569~574 (2007), 한국식품과학회
- 이미숙, "한국산 5종 해조류의 항산화효과와 총 폴리페놀 함량과의 관련성", 한국식품영양과학회지, 34(8), pp.1143~1150 (2005), 한국식품영양과학회
- Kang, Y.H., "The nitrite scavenging and electron donating ability of phenolic compounds.", Korean J. Food Sci. Technol, 28(2), pp.232~236 (1996)
- Fresco, P., "New insights on the anticancer properties of dietary polyphenols", J. Med. Res Reviews, 26, pp.747~766 (2006)
- 김현구, "마이크로웨이브 추출공정에 의한 팽이버섯 추출물의 기능적 특성", 한국식품과학회지, 34(6), pp.1013~1017 (2002), 한국식품과학회
- 이양숙, "가죽나무(Ailanthus altissima) 열수 추출물의 생리활성", 한국식품저장유통학회지, 14(2), pp.170~176 (2007), 한국식품저장유통학회
- 김현우, "황벽나무, 두충나무 등을 포함하는 복합수목추출물의 항균활성 및 안전성 시험", Korean Chemical Engineering Research(HWAHAK KONGHAK), 51(5), pp.536~539 (2013), 한국화학공학회[DOI]
- 박혜미, "추출방법에 따른 상지 추출물의 항산화 활성", 한국식품영양과학회지, 43(11), pp.1709~1715 (2014), 한국식품영양과학회
- 조영제, "복분자(Rubus coreanum F.) 열수 및 에탄올추출물의 생리활성비교", 한국식품영양과학회지, 34(6), pp.790~796 (2005), 한국식품영양과학회
- 황혜진, "유근피 추출물이 대식세포 면역조절에 미치는 영향", 생명과학회지, 26(1), pp.50~58 (2016), 한국생명과학회
- Locksley, R. M., "The TNF and TNF receptor superfamilies: integrating mammalian biology", Cell, 104, pp.487~501 (2001)
- Lee ES, "Inhibition of nitric oxide and tumor necrosis factor-α (TNF-α) production by propenone compound through blockade of unclear factor (NF)-kB activation in cultured Murine macrophages", Biol Pharm Bull, 27, pp.617~620 (2004)
- Gasparini, C., "NF-κ B as a target for modulating inflammatory responses", Curr. Pharm. Des, 18, pp.5735~5745 (2012)
- Murray, P. J., "Protective and pathogenic functions of macrophage subsets", Nat. Rev. Immunol, 11, pp.723~737 (2011)
- Zhou, D., "Macrophage polarization and function with emphasis on the evolving roles of coordinated regulation of cellular signaling pathways", Cell Signal, 26, pp.192~197 (2014)