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Evaluation of Whitening and Antibacterial Efficacy using Chamaecyparis obtusa (Siebold & Zucc.) Endl Root : Focusing on Functional Cosmetic Materials

권용진|박시현
한국동물실험대체법학회 (Korean Society for Alternatives to Animal Experiments)|2024.12|한국동물실험대체법학회지 (Journal of Alternatives to Animal Experiments)|제18권 제2호|pp. 27-36

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키워드

항균뿌리화장품 소재미백Cosmetic materialsWhitening effectsChamaecyparis obtusa (Siebold & Zucc.) EndlRootsAntibacterial effects

초록

편백나무 (Chamaecyparis obtusa (Siebold & Zucc.) Endl.) 추출물은 여러 종류의 테르펜 성분을 포함하고 있으며, 편백나무의 잎과 가지에서 얻은 정유는 항균, 항진균, 항염증 효과에 관한 연구 결과들이 보고되고 있다. 현재 편백나무의 잎, 수피 등을 활용한 화장품 소재 활성 평가에 관한 연구는 많이 진행되고 있으나, 뿌리를 이용한 연구는 부족한 상태이다. 본 연구에서는 편백나무 뿌리의 열수 추출물 (CORW)과 70% 에탄올 추출물 (CORE)을 이용하여 미백, 항산화, 세포 미백, 항균 실험을 진행하였다. CORW와 CORE를 이용하여 tyrosinase 저해 활성과 ABTS+ radical 소거능을 확인한 결과 CORE가 더 우수한 효능을 보였다. 추가로 α-MSH 유도 B16F10 모델을 이용하여 CORE의 세포내 미백 활성을 분석하였다. CORE 시료는 α-MSH에 의해 유도되는 멜라닌 함량과 세포내 tyrosinase 활성을 상당히 억제하였다. 또한 화장품에서 검출이 제한된 4종인 S. epidermidis, S. aureus, E. coli, P. aeruginosa와 여드름 균인 C. acnes를 사용하여 항균 효능을 확인한 결과, 모두 우수한 항균 효능을 보였다. 결론적으로 본 연구에서 CORE의 미백 및 항균 효과를 확인하였기에 기능성 화장품 소재로서 적용이 가능할 것으로 시사된다.

Abstract

Chamaecyparis obtusa (Siebold & Zucc.) Endl. (C. obtusa) extract contains many kinds of terpene components, and studies on antimicrobial, antifungal, and anti-inflammatory effects have been reported using essential oil of leaves and branches of cypress trees. Currently, there are many studies on evaluating the activity of cosmetic materials using leaves and barks of C. obtusa, but studies using roots of C. obtusa are not well known yet. In this study, whitening, antioxidant, and antibacterial experiments were conducted using boiling water extract of C. obtusa root (CORW) and 70% ethanol extract of C. obtusa root (CORE). As a result of confirming the tyrosinase inhibition activity and ABTS+ radical scavenging activity, CORE showed better whitening and antioxidant efficacy than CORW. To confirm additional whitening efficacy, the α-MSH-induced B16F10 model was used. CORE significantly inhibits α-MSH-induced melanin contents and intracellular tyrosinase activitiy in B16F10 cells. In addition, as a result of confirming antibacterial efficacy using S. epidermidis, S. aureus, E. coli, and P. aeruginosa, and C. acnes, CORE showed excellent antibacterial efficacy in all strains. In conclusion, since the excellent whitening and antibacterial effects of CORE were confirmed in this study, CORE could be applied as a functional cosmetic material.

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