꼬리진달래 신초의 폴리페놀 프로파일링과 추출용매에 따른 유용성분 함량 및 항산화 활성 비교
Polyphenol Profiling and Comparison of Extraction Solvents on Useful Component Content and Antioxidant Activity of Rhododendron micranthum Shoot
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초록
본 연구는 꼬리진달래의 자원식물 개발을 위한 정보를 제공하고자 수행되었다. 꼬리진달래 신초 추출물의 폴리페놀 프로파일링을 통해 유용성분을 선발하고, 추출용매에 따른 유용성분 함량과 항산화 활성을 비교하였다. 꼬리진달래 신초 추출물에서 37개의 폴리페놀 화합물을 확인하였으며, chlorogenic acid, astragalin, myricetin, afzelin의 추출용매별 함량을 비교한 결과, chlorogenic acid의 함량은 에탄올 추출물에서6.57±0.12 ㎎/g이었고, astragalin의 함량은 에탄올 추출물에서 2.29±0.02 ㎎/g, myricetin의 함량은 메탄올 추출물에서4.77±0.06 ㎎/g, afzelin의 함량은 메탄올 추출물에서 0.10 ±0.01 ㎎/g이었다. 꼬리진달래 에탄올 추출물의 총 폴리페놀함량은 80.01±2.36 ㎎/g, 총 플라보노이드 함량은 78.08± 3.44 ㎎/g이었다. 또한 꼬리진달래 메탄올 추출물의 DPPH radical 소거활성 IC50값은 943.57±10.68 ㎎/L였으며, ABTS radical 소거활성 IC50은 641.60±7.58 ㎎/L였다. 꼬리진달래는유기용매 추출 시 유용성분과 항산화 활성이 높았고, 동일 속의종들과 비교하여 높은 함량의 chlorogenic acid, astragalin, myricetin, afzelin을 함유하고 있어 향후 건강기능성 소재로의개발이 가능할 것으로 판단된다.
Abstract
This study was conducted to provide information on the possibility of using Rhododendron micranthum as resource plant. Through polyphenol profiling of R. micranthum shoot extract, useful components were selected and the content of useful components and antioxidant activity by extraction solvent were compared. As a result, 37 polyphenol compounds were identified in R. micranthum shoot extract through LC-ESI-MS analysis. Contents by extraction solvent were compared of chlorogenic acid, astragalin, myricetin, and afzelin. Chlorogenic acid was highest at 6.57±0.12 ㎎/g in the ethanol extract, astragalin was 2.29±0.02 ㎎/g in the ethanol extract, myricetin was 4.77±0.06 and 4.52±0.27 ㎎/g in the methanol, ethanol extracts, and afzelin was highest at 0.10±0.01 ㎎/g in the methanol extract. Total polyphenol content of the R. micranthum shoot extract according to the extraction solvent was highest in the ethanol extract at 80.01±2.36 ㎎/g, and total flavonoid content was also highest in the ethanol extract at 78.08±3.44 ㎎/g. The IC50 value of DPPH radical scavenging activity according to the extraction solvent of R. micranthum shoot was lowest in methanol extract at 943.57±10.68 ㎎/L, and the IC50 value of ABTS radical scavenging activity was also lowest in methanol extract at 641.60±7.58 ㎎/L.
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