꼬리진달래의 대량증식을 위한 기내배양 조건 확립 및 배양 단계별 유용성분 함량 분석
Establishment of in vitro culture conditions for mass-production and analysis of functional compound contents at culture stage in Rhododendron micranthum Turcz.
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In this study, suitable culture media, growth regulators, and acclimatization soil for regenerated plants were evaluated to enable mass production of Rhododendron micranthum, and the contents of functional compounds at culture stage were analyzed to confirm industrial applicability. For shoot proliferation, culturing in 4 mg/L zeatin-containing Murashige and Skoog medium was found to be the most effective treatment; the shoot induction rate was 77.08%, number of developing shoots was 2.8, and length of shoots was 9.01 mm. Regarding culture medium and growth regulator suitable for rooting, half-strength Economou and Read medium and 1 mg/L indole-3-butyric acid treatment were the most effective, with a rooting rate of 95.83%, root number of 4.1, and root length of 12.59 mm. Peatmoss was found to be most effective for facilitating ex vitro accli- matization of regenerated plants, with a seedling shoot length of 69.27 mm and root length of 49.30 mm. Chlorogenic acid, astragalin, myricetin, and afzelin contents were analyzed via high-performance liquid chromatography analysis at culture stage. The in vitro-rooted seedlings contained 3.77 mg/g chlorogenic acid, 1.53 mg/g astragalin, 1.07 mg/g myricetin, and 0.17 mg/g afzelin; the contents were higher than those in in vitro shoots (0.44 mg/g chlorogenic acid, 1.30 mg/g astragalin, 1.04 mg/g myricetin, and 0.05 mg/g afzelin) and increased as the stage progressed. We confirmed that these culture conditions can be used for additional research, such as increasing the contents of functional compounds and mass production research. Based on these findings, there is a possibility of supplying raw materials and producting four functional compound using in vitro culture of R. micranthum.
Abstract
In this study, suitable culture media, growth regulators, and acclimatization soil for regenerated plants were evaluated to enable mass production of Rhododendron micranthum, and the contents of functional compounds at culture stage were analyzed to confirm industrial applicability. For shoot proliferation, culturing in 4 mg/L zeatin-containing Murashige and Skoog medium was found to be the most effective treatment; the shoot induction rate was 77.08%, number of developing shoots was 2.8, and length of shoots was 9.01 mm. Regarding culture medium and growth regulator suitable for rooting, half-strength Economou and Read medium and 1 mg/L indole-3-butyric acid treatment were the most effective, with a rooting rate of 95.83%, root number of 4.1, and root length of 12.59 mm. Peatmoss was found to be most effective for facilitating ex vitro accli- matization of regenerated plants, with a seedling shoot length of 69.27 mm and root length of 49.30 mm. Chlorogenic acid, astragalin, myricetin, and afzelin contents were analyzed via high-performance liquid chromatography analysis at culture stage. The in vitro-rooted seedlings contained 3.77 mg/g chlorogenic acid, 1.53 mg/g astragalin, 1.07 mg/g myricetin, and 0.17 mg/g afzelin; the contents were higher than those in in vitro shoots (0.44 mg/g chlorogenic acid, 1.30 mg/g astragalin, 1.04 mg/g myricetin, and 0.05 mg/g afzelin) and increased as the stage progressed. We confirmed that these culture conditions can be used for additional research, such as increasing the contents of functional compounds and mass production research. Based on these findings, there is a possibility of supplying raw materials and producting four functional compound using in vitro culture of R. micranthum.
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