진달래꽃 추출물의 항산화 효과 및 인체 KB cell에 대한 세포독성
Antioxidative Activity and Cytotoxicity on Human KB cell of Extracts from Rhododendron mucronulatum Turcz. Flower
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진달래꽃 추출물의 항산화 효과와 인체 비인두암 세포인 KB cell에 대한 세포독성을 조사하였다. 또한 총 폴리페놀과 총 플라보노이드 함량을 측정하고 항산화성과의 상관성을 검토하였다. 진달래꽃 메탄올 추출물을 용매 분획하여 항산화 활성을 측정한 결과, 에칠아세테이트 분획의 활성이 가장 높게 나타났다. 에칠아세테이트 분획을 silica gel column chromatography로 부분 정제하여 항산화 실험을 한 결과 fraction 2와 3은 40 ppm 농도에서 90% 이상의 활성을 나타내었다. 인체 비인두암 세포주인 KB cell의 성장 저해율은 100 μg/mL 농도에서 클로로포름 분획이 81.2%, 헥산 분획이 74.6% 였다. 총 폴리페놀과 총 플라보노이드 함량을 측정한 결과, 에칠아세테이트 분획에는 총 폴리페놀 32.70%, 총 플라보노이드 20.30% 함유하고 있었다. 총 폴리페놀 및 총 플라보노이드 함량과 항산화성과의 상관관계는 총 폴리페놀에 대하여는 r2=0.8869, 총 플라보노이드에 대하여는 r2=0.8469로 상관성이 인정되었다.
Abstract
This study was carried out to investigate the antioxidative activity and cytotoxicity on human KB cell of extract from Rhododendron mucronulatum flower, and also to determine the contents of total polyphenol and flavonoid. The methanol extract of Rhododendron mucronulatum flower was fractionated with various solvents such as hexane, chloroform, ethyl acetate, butanol and water. The DPPH radical scavenging activities of ethyl acetate fraction had stronger activity than other fractions. So, antioxidative substances of ethyl acetate fraction were crude purified by silica gel column chromatography. The DPPH radical scavenging activities of crude purified fraction 2 and 3 were more than 90% at 40 ppm. In the presence of 100 μg/mL, growth inhibition on human KB cell by WST-1 assay showed 81.2% in chloroform fraction and 74.6% in hexane fraction. The contents of total polyphenol and flavonoid of ethyl acetate fraction were 32.70% and 20.30%, respectively. The antioxidative activity showed correlation with total polyphenol and flavonoid contents.
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