로즈마리 추출물의 화장품 생리활성 및 세포 독성 연구
Study of Cosmetic Biological Activity and Cytotoxicity on Rosemary Extract
0
조회
0
다운로드
14
인용
0
참고문헌
키워드
초록
This study of the antioxidant effect of rosemary extract and anti-inflammatory,anti-bacterial effect to find out, it is harmless to the human body, and antioxidants for aging and skin whitening evaluate the effect is higher. Thus, the DPPH radical scavenging antioxidant effect and ABTS radical scavenging activity and polyphenol, flavonoid content was measured, anti-inflammatory, antibacterial,cytotoxic effects were measured. As a result, According to the measurement of the DPPH radical scavenging activity, as the concentration of rosemary extract increased in a concentration-dependent. And an analysis of the ABTS radical scavenging activity of rosemary extract, it increased in a concentration-dependent. The total polyphenol content in 1㎎/㎖ was ±0.056㎎(GAE)/100g while flavonoid content was 175.1±0.020㎎(GAE)/100g. The results, it was verified that rosemary has strong anti-oxidant effects. The extract also revealed strong anti-microbial effects against Propionibacterium acne and Staphylococcus aureus. According to analysis of the vitality of mouse macrophages in rosemary extract, high cell vitality was observed at low concentrations. In this study,therefore, the availability of rosemary extract as a cosmetics material was confirmed. It is expected that the results of the study would be useful as basic data in the manufacture of cosmetics and cosmetics-related studies.
Abstract
This study of the antioxidant effect of rosemary extract and anti-inflammatory,anti-bacterial effect to find out, it is harmless to the human body, and antioxidants for aging and skin whitening evaluate the effect is higher. Thus, the DPPH radical scavenging antioxidant effect and ABTS radical scavenging activity and polyphenol, flavonoid content was measured, anti-inflammatory, antibacterial,cytotoxic effects were measured. As a result, According to the measurement of the DPPH radical scavenging activity, as the concentration of rosemary extract increased in a concentration-dependent. And an analysis of the ABTS radical scavenging activity of rosemary extract, it increased in a concentration-dependent. The total polyphenol content in 1㎎/㎖ was ±0.056㎎(GAE)/100g while flavonoid content was 175.1±0.020㎎(GAE)/100g. The results, it was verified that rosemary has strong anti-oxidant effects. The extract also revealed strong anti-microbial effects against Propionibacterium acne and Staphylococcus aureus. According to analysis of the vitality of mouse macrophages in rosemary extract, high cell vitality was observed at low concentrations. In this study,therefore, the availability of rosemary extract as a cosmetics material was confirmed. It is expected that the results of the study would be useful as basic data in the manufacture of cosmetics and cosmetics-related studies.
참고문헌 (40)
- 권계영, "대황추출물의 항산화 효과 및 생리활성에 관한 연구", 석사, 미용교육, pp.2011
- 장아람, "무궁화 (Hibiscus syriacus) 추출물의 화장품 생리 및 피부세포 활성 연구", 석사, 향장미용학과, pp.2012
- 잔가시모자반에서새로이 밝혀진 신물질, "국립생물자원관", 한국개발연구원, 1~6 (2011)
- 박주아, "소리쟁이(Rumex crispus L.) 잎 추출물의 항산화 효과 및 각질세포 보호효과", 한국인체미용예술학회지, 12(2), pp.189~198 (2011), 한국인체미용예술학회
- 이응룡, "플라보노이드의 세포 신호전달물질", 충남대학교 논문집, 1, pp.572~592 (2007)
- 윤미연, "질경이 seed 추출물의 항산화 활성 및 미백작용에 관한 연구", 한국인체미용예술학회지, 14(2), pp.259~269 (2013), 한국인체미용예술학회
- Caldefied-Chezet, F., "Anti-inflammatory effects of melaleuca altermifolia essential oil on human polymorphonuclear neutrophils and monocytes", Free Radic. Res, 38, pp.805~811 (2004)
- Medeiros, R, "Effect of two active compounds obtained from the essential oil of Cordia verbenacea on the acute inflammatory responses elicited by LPS in the rat paw", Br. J. Pharmacol, 151, pp.618~627 (2007)
- Sylvestre, M, "Essential oil analysis and anticancer activity of leaf essential oil of Croton Flavens L. Form Guadeloupe", J. Ethnopharmacol, 103, pp.99~102 (2006)
- Misaghi, A., "Effects of Zataria Multiflora boiss. essential oil and nisin on Bacillus cereus ATCC 11778", Food Control, 18, pp.1043~1049 (2007)
- 오유정, "로즈마리 추출액을 이용한 직물의 염색", 석사, 의류학, pp.2001
- 임연실, "로즈마리차 섭취와 정유흡입이 흡연 대학생의 항산화능에 미치는 영향", 박사, 식품영양학, pp.2011
- Okamura N, "Flavonoid in Rosemarinus Officinalis Leaves", Phytochemistry, 37(5), pp.1463~1467 (1994)
- Frankel ENand Huang SW, "Antioxidant Activity of a Rpsemary Extract and Its Constituents, Caronosol Acid, Carnosol, and Rosemarinic Acid, in Bulk Oil, Oil-in Water Emulsion", J. Agric. Food Chem, 44, pp.131~135 (1996)
- Nakatani N, "Structure of Rosemanol, A New Antioxidani fron Rosemary", Agricultural and Biological Chemistry, 45(10), pp.2366~2385 (1981)
- Blios MS, "Antioxidant determination by the use of a stable free radical", Nature, 46(17), pp.1198~1199 (1958)
- Van den Berg R, "Applicability of an improved Trolox equivalent anti-oxidant capacity (TEAC) assay for evaluation of antioxidant capacity measurements of mixture", Food Chem, 66, pp.511~517 (1999)
- Dewanto V, "Processed sweet corn has higher antioxidant activity", J. Agric Food Chem, 50, pp.4959~4964 (2002)
- Zia Z, "The determination of flavonoid contents in m㎕berry and their scavenging effect on superoxide radicals", Food Chem, 64, pp.555~559 (1999)
- 이철호, "식품저장학", pp.174~ (2008), 고려대학교출판부
- 권은경, "9종의 허브류로부터ACE저해활성,HMG-CoA reductase저해활성 및혈전용해활성에 대한 검색", 한국식품과학회지, 38(5), pp.691~698 (2006), 한국식품과학회
- 고경숙, "황금(黃芩) 추출물의 항산화 활성 및 세포독성 연구", 한국인체미용예술학회지, 14(2), pp.233~243 (2013), 한국인체미용예술학회
- 김혜진, "맹종죽, 왕대, 솜대 죽순 추출물의 생리활성에 관한 연구", 석사, 산림자원학과 산림자원학전공, pp.2012
- 이승욱, "울릉도산 산채류 추출물의 총 폴리페놀 함량 및 항산화 활성", 한국식품과학회지, 37(2), pp.233~240 (2005), 한국식품과학회
- 최준혁, "로즈마리(Rosmarinus officinalis L.)의 염증억제 및 항통풍 효과", 석사, 식품가공학, pp.2010
- 채인경, "국내 시판되는 허브류의 항산화 및 항균효과 검색", 한국식품영양과학회지, 39(10), pp.1411~1417 (2010), 한국식품영양과학회
- 동석, "정향추출물의 항산화 효과", 한국식품조리과학회학술발표자료, pp.21~ (2003)
- 김남우, "뜰보리수 열매의 유용성분 분석", 한국식품저장유통학회지, 10(4), pp.534~539 (2003), 한국식품저장유통학회
- 유자연, "제천 싸리나무 열수추출물의 항산화, 항균 및 Tyrosinase 저해활성", 석사, 한방식품영양학, pp.2011
- Havsteen B, "Flavonoids a class of natural products of high pharmcological potency", Biochem Pharmacol, 3, pp.1141~1148 (1983)
- 이윤경, "마늘의 기능성 품질과 토양 성분 간의 관계", 한국식품과학회지, 40(1), pp.31~35 (2008), 한국식품과학회
- 김혜란, "한재 미나리의 건조속도와 분말의 흡습특성 및 기능성에 관한 연구", 석사, 식품공학, pp.2008
- 서영옥, "삼백초 추출물의 항균성과 두피에 미치는 영향", 한국인체미용예술학회지, 14(2), pp.103~112 (2013), 한국인체미용예술학회
- 김기은, "두충나무, 황벽나무 등을 포함하는 수목추출물의 항여드름 및 항아토피 효과", Korean Chemical Engineering Research(HWAHAK KONGHAK), 48(6), pp.700~703 (2010), 한국화학공학회
- Burton JL, "The relationship between seborrhea and acne vulgaris", British Journal of Dermatology, 84, pp.600~601 (1971)
- Cunliffe WJ, "comedone formation: etiology, clinical presentation and treatment", Clinics in Dermatology, 22, pp.367~374 (2004)
- 오수정, "울금 및 발효울금 추출물의 피부관련 생리활성 탐색", 한국인체미용예술학회지, 14(2), pp.185~195 (2013), 한국인체미용예술학회
- 양선아, "산벚나무 잎 추출물 및 분획물의 항균활성과 Nitric Oxide 생성억제 활성", 한국약용작물학회지, 20(5), pp.308~314 (2012), 한국약용작물학회
- 이미경, "사철쑥 추출물의 면역세포의 생육증진 및 세포독성", 한국약용작물학회지, 12(1), pp.36~42 (2004), 한국약용작물학회
- Jung Ae Park, "머루근 추출물 및 분획물의 항비만 활성", 생명과학회지, 23(1), pp.69~78 (2013), 한국생명과학회[DOI]