동의보감 무운산 처방 추출물의 생리활성 비교
Comparative Analysis of Physiological Activities of Muunsan Extract from the Donguibogam
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
This study investigated the usability of muunsan in the Donguibogam (Korean Medical Encyclopedia) as a cosmetic material by analyzing its physiological activities. In terms of total phenol contents, MUSD and MUSE were 234.34±0.59 ㎎/g and 429.21±1.55 ㎎/g, respectively. It was confirmed that it has poly-phenolic compounds, which are similar to the reference material ‘tannic acid’. According to the measurement of antioxidant efficacies, MUSD and MUSE were 89% and 96% each at 500㎍/㎖ in terms of DPPH radical scavenging activity. In terms of ABTS radical scavenging activity, MUSD and MUSE were 70% or higher and 80% or higher at 1,000㎍/㎖, showing antioxidant effects similarly to that of Vit-C. In ROS production, MUSD and MUSE revealed a significant decrease (***p<0.001, **p<0.01, *p<0.05) at 50, 100 ㎍/㎖ and 10, 50, 100 ㎍/㎖ respectively. In cytotoxicity, cell viability was 85% or higher up to 500 ㎍/㎖, confirming no toxicity. In terms anti-inflammatory effects, nitric oxide production decreased up to 500 ㎍/㎖. According to a western blot, when the extract was processed by concentration, protein expression declined in a dose-dependent manner. In antimicrobial activity, as the extract got more concentrated, antimicrobial activity increased, confirming antimicrobial effects. The above results show that muunsan extract can be used in manufacturing cosmetics as a natural substance with antioxidant, anti-inflammatory and antimicrobial effects.
Abstract
This study investigated the usability of muunsan in the Donguibogam (Korean Medical Encyclopedia) as a cosmetic material by analyzing its physiological activities. In terms of total phenol contents, MUSD and MUSE were 234.34±0.59 ㎎/g and 429.21±1.55 ㎎/g, respectively. It was confirmed that it has poly-phenolic compounds, which are similar to the reference material ‘tannic acid’. According to the measurement of antioxidant efficacies, MUSD and MUSE were 89% and 96% each at 500㎍/㎖ in terms of DPPH radical scavenging activity. In terms of ABTS radical scavenging activity, MUSD and MUSE were 70% or higher and 80% or higher at 1,000㎍/㎖, showing antioxidant effects similarly to that of Vit-C. In ROS production, MUSD and MUSE revealed a significant decrease (***p<0.001, **p<0.01, *p<0.05) at 50, 100 ㎍/㎖ and 10, 50, 100 ㎍/㎖ respectively. In cytotoxicity, cell viability was 85% or higher up to 500 ㎍/㎖, confirming no toxicity. In terms anti-inflammatory effects, nitric oxide production decreased up to 500 ㎍/㎖. According to a western blot, when the extract was processed by concentration, protein expression declined in a dose-dependent manner. In antimicrobial activity, as the extract got more concentrated, antimicrobial activity increased, confirming antimicrobial effects. The above results show that muunsan extract can be used in manufacturing cosmetics as a natural substance with antioxidant, anti-inflammatory and antimicrobial effects.
참고문헌 (25)
- 김계순, "갯버들 추출물의 두피 기능성화장품 소재개발 연구", 박사, 향장미용학과, pp.2017
- 김형우, "포공영 추출물의 항산화 효과 및 피부 각질세포 보호효과", 대한본초학회지, 24(3), pp.103~108 (2009), 대한본초학회
- 김란, "한방허브추출물을 첨가한 기본제형 크림에 의한 피부보습상태 변화 연구", 아시안뷰티화장품학술지, 10(2), pp.399~404 (2012), 한국피부과학연구원[DOI]
- 장영아, "한방 복합추출물이 알레르기성 접촉피부염과 항염증에 미치는 영향", 대한본초학회지, 29(2), pp.55~60 (2014), 대한본초학회
- 김아름, "오배자(Gallnut) 열수 추출물의모발염색 및 염색 견뢰도", 석사, pp.2011
- 한갑훈, "세신 정유 추출물의 피부진균 및 기회감염진균에 대한 항진균 효과", 한국균학회지, 35(1), pp.58~60 (2007), 한국균학회
- 정의영, "여드름 치료용 크림 조성물", 특허출원번호: 10-2011-0077279, ~ (2011)
- 허은정, "치매 치료용 한약 처방의 연구성과에 대한 정성평가", 동의신경정신과학회지, 23(1), pp.93~113 (2012), 대한한방신경정신과학회[DOI]
- 도의정, "천연보존제를위한 특정 한방처방의추출용매에 따른 항균활성 비교", 한약정보연구회지, 3(1), pp.1~6 (2015)
- 김계숙, "도시여성의 한방화장품 사용에 대한 만족도와 구매특성에 관한 연구", 한국미용학회지, 14(4), pp.1443~1459 (2008), 한국미용학회
- O. Folin, "On phosphotungsticphosphomo-lybdic compounds as color reagents", J Biol Chem, 12, pp.239~243 (1912)
- M. S. Blois, "Antioxidant determinations by the use of a stable free radical", Nature, 181(4617), pp.1199~1200 (1958)
- P. Nicoletta., "Screening of dietary carotenoids and carotenoid-rich fruit extracts for antioxidant activities applying 2, 2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)radical cation decolorization assay", Methods Enzymol, 299, pp.379~389 (1999)
- W. Alassane, "Annomuricatin C, a novel cyclohexapeptide from the seeds of Annona muricata", Comptes Rendus Chimie, 7(10), pp.981~988 (2004)
- J. Carmichael, "Evaluation of a tetrazolium based semiautomated colorimetric assay : assessment of radiosensitivity", Cancer Res, 47(4), pp.943~946 (1987)
- 김소은, "화장품 소재로서 세발나물 추출물의 생리활성 연구", 한국인체미용예술학회지, 20(2), pp.59~71 (2019), 한국인체미용예술학회[DOI]
- 이민영, "과일 껍질의 비타민 C , 폴리페놀, 플라보노이드 함량과 항산화 활성", 한국식품과학회지, 44(5), pp.540~544 (2012), 한국식품과학회[DOI]
- 이헌호, "재배종 및 야생 산딸기의 기능성 성분 함량과 항산화 활성", 원예과학기술지, 32(1), pp.115~122 (2014), 한국원예학회[DOI]
- 김정환, "진범(Aconitum pesudo-laeve var. erectum) 추출물의 항노화 및 항당뇨 효과", 생명과학회지, 23(5), pp.616~621 (2013), 한국생명과학회[DOI]
- A. Koreck, "The role of innate immunity in the pathogenesisof acne", Dermatol, 206(2), pp.96~105 (2003)
- 유민정, "상백피 추출물의 피부상재균에 대한 항균활성과 항산화 효과", 한국인체미용예술학회지, 11(2), pp.87~98 (2010), 한국인체미용예술학회
- 전형주, "명월초(Gynura Procumbens)의성분분석과 화장품 및 식품 소재로서 유효성평가", 박사, pp.2015
- Trung Kien Nguyen, "갈색먹물버섯 자실체의 메탄올과 열수추출물의 항산화, 항당뇨, 항콜린에스테라아제, 항티로시나아제 및 Nitric oxide의 저해 효과", 한국버섯학회지, 12(4), pp.330~340 (2014), 한국버섯학회[DOI]
- 김정환, "둥근마 에탄올 추출물의 항염증 효과", 한국인체미용예술학회지, 17(1), pp.91~102 (2016), 한국인체미용예술학회
- 이진영, "채진목 에탄올 추출물의 항염증 효과 검증", 대한화장품학회지, 43(1), pp.19~26 (2017), 사단법인 대한화장품학회