황칠나무 잎 추출물의 기능성 화장품 및 육모제 소재 활용 연구
A Study on the Utilization of Dendropanax morbifera Lev. Leaf Extract for Material of Functional Cosmetics and Hair Growth Products
2
조회
0
다운로드
13
인용
0
참고문헌
키워드
초록
목적: 본 연구는 황칠나무(Dendropanax morbifera) 잎에서 메탄올-초음파 추출법으로 유효성분을 추출하여 약물요법을 대체 할 수 있는 기능성 화장품 및 육모제 소재 개발에 자료를 제공하고자 하는 목적으로 수행하였다. 방법: 황칠나무 잎 메탄올-초음파 추출물의 총 폴리페놀 및 플라보노이드 함량을 측정하였고, 항산화능과 아질산염 소거능을 측정하였으며, 항균활성과 최소성장저해농도(minimum inhibitory concentration, MIC)를 측정하였다. HFDPC세포에 대하여 10, 100, 500 μg/mL 농도별로 처리하여 MTT assay를 통하여 세포독성을 평가하였으며, western blotting을 통한 성장인자 발현촉진 및 억제 효과를 측정하였다. 결과: 황칠나무 잎 메탄올-초음파 추출물의 총 폴리페놀 함량은 74.082±7.180 mg/g, 총 플라보노이드 함량은 97.360±2.235 mg/g로 나타났다. DPPH 소거능은 92.440±1.023%로 높은 효과를 보였으며, 아질산염 소거능은 52.6±2.4%를 보였다. Propionibacterium acnes (P. acnes), Pityrosporum ovale (P. ovale), Malassezia furfur (M. furfur) 균주에 대하여 항균활성이 나타났으며, 최소성장저해농도(MIC)는 P. acnes에서는 17.3%, P. ovale는 21.6%, M. furfur 에서는15.7% 농도로 나타났다. 인간모유두세포에 황칠나무 추출물을 10, 100, 500 μg/mL의 처리농도 범위 내에서세포독성은 관찰되지 않았으며, 농도가 증가할수록 세포생육도가 높았다. Western blotting을 통한 성장인자 단백질의 발현및 억제 효과는 대조군 대비 IGF는 156±8%로 발현촉진 효과가, TGFβ2는78.3±10%로 억제효과가 나타났다. 결론: 황칠나무 잎 메탄올-초음파 추출물은 항산화능을 가진 기능성 화장품 및 육모제의 소재로 활용가능성이 있는 것으로 사료된다.
Abstract
Purpose: This study was conducted to provide data of the material development of functional cosmetics and hair growth products that could replace the drug therapies by extracting the active ingredients from Dendropanax morbifera (D. morbifera) leaf using the methanol-ultrasound extraction method. Methods: Total polyphenol and flavonoid contents of D. morbifera leaf methanol-ultrasound extract were measured. Also, the anti-oxidant capacity, nitrite scavenging ability, anti-microbial activity, and the minimum inhibitory concentration (MIC) were measured. Cytotoxicity of the extract on HFDPCs was examined by MTT assay at concentrations of 10, 100, and 500 μg/mL, respectively. Moreover, the promotive and inhibitory effects of growth factor expression were also confirmed through the western blotting. Results: Total polyphenol and flavonoid contents of D. morbifera leaf methanol-ultrasound extract were 74.082±7.180 mg/g and 97.360±2.235 mg/g, respectively. DPPH scavenging ability was 92.440±1.023%, which was very high, and the nitrite scavenging activity was 52.6±2.4%. Anti-microbial activities were investigated in the strains such as Propionibacterium acnes (P. acnes), Pityrosporum ovale (P. ovale), and Malassezia furfur (M. furfur). The MIC of P. acnes, P. ovale, and M. furfur were 17.3%, 21.6%, and 15.7%, respectively. Cytotoxicity was not observed within the range of concentrations (10, 100, and 500 μg/mL) of D. morbifera leaf methanol-ultrasound extract and the cell growth rate was increased according to the concentrations. In case of the growth factor expression by western blotting, high promotive effect was observed in IGF protein expression (156±8%) compared with the control group, while inhibitory effect was confirmed in TGFβ2 protein expression (78.3±10%). Conclusion: Therefore, it is possible to utilize D. morbifera leaf methanol-ultrasound extract as the material of functional cosmetics and hair growth products with anti-oxidant capacity.
참고문헌 (33)
- 배기화, "황칠나무(Dendropanax morbifera)의 부정근 유도 및 기내증식조건", Journal of Plant Biotechnology, 36(2), pp.163~169 (2009), 한국식물생명공학회
- Bartsch H, "Inhibitors of endogenous nitrosation: mechanisms and implications in human cancer prevention", Mutation Research, 202, pp.307~324 (1988)
- Bauer AW, "Antibiotic susceptibility testing by a standardized single disk method", American Journal of Clinical Pathology, 45, pp.493~496 (1966)
- 최문희, "동백나무 잎 추출물의 여드름 피부 개선 효과", 아시안뷰티화장품학술지, 10(3), pp.661~672 (2012), 한국피부과학연구원
- 최성규, "완도군 황칠나무의 생육특성", 한국작물학회지, 48(5), pp.434~437 (2003), 한국작물학회
- Foitzik K, "Towards dissecting the pathogenesis of retinoidinduced hair loss: all-trans retinoic acid induces premature hair follicle regression (catagen) by upregulation of transforming growth factor-beta2in the dermal papilla", Journal of Investigative Dermatology, 124, pp.1119~1126 (2005)
- Folin O, "On phosphotungstic-phosphomolybdic compounds as color reagents", Journal of Biological Chemistry, 12, pp.239~243 (1912)
- Gray JI, "Inhibition of N-nitrosamine formation in model food systems", Journal of Food Science, 40, pp.981~984 (1975)
- 한은경, "민들레의 부위별 에탄올 추출물의 항산화 활성", 한국식품영양과학회지, 40(1), pp.56~62 (2011), 한국식품영양과학회
- Hibino T, "Role of TGF-beta2 in the human hair cycle", Journal of Dermatological Science, 35, pp.9~18 (2004)
- 형순희, "C57BL", 6 마우스 모델에서 복합 한약추출물의 모발성장 촉진효과, 2007, pp.13(3), 1366~1375[DOI]
- Jamora C, "A signaling pathway involving TGFbeta2and snail in hair follicle morphogenesis", PLoS Biology, 3, pp.e11~ (2005)
- 김은희, "두피모발관리 시설에 따른 이용실태 및 만족도 비교연구", 한국인체미용예술학회지, 15(3), pp.59~68 (2014), 한국인체미용예술학회
- 김은진, "자생식물과 생약자원 추출물의 폴리페놀, 플라보노이드 함량 및 항산화 활성 탐색", 한국식품과학회지, 44(3), pp.337~342 (2012), 한국식품과학회[DOI]
- 김경동, "약용식물의 부위별 및 추출용매에 따른 효능 연구", 대한화장품학회지, 33(2), pp.127~135 (2007), 대한화장품학회
- Kim SM, "The antioxidant ability and nitrite scavenging ability of plant extracts", Korean Journal of Food Science and Technology, 33, pp.626~632 (2001)
- Kwack MH, "Dickkopf 1promotes regression of hair follicles", Journal of Investigative Dermatology, 132, pp.1554~1560 (2012)
- 권민철, "병풀의 초음파 추출 시 용매에 따른 면역활성 증진 효과", 한국약용작물학회지, 16(5), pp.294~300 (2008), 한국약용작물학회
- Kwon OS, "Human hair growth enhancement in vitro by green tea epigallocatechin-3-gallate", Phytomedicine, 14, pp.551~555 (2007)
- 이혜숙, "C57BL", 6마우스 모델에서 DCS-HT® 발모제의 모발성장 촉진 효과, 2009, pp.7(3), 131~142[DOI]
- Lee MK, "For the utilization of native plant resources as high-value materials: evaluation on demelanizing activity of Dendropanax morbifera in Bogildo", Journal of Korean lsland, 25, pp.227~240 (2013)
- Lin WH, "Fibroblast growth factors stimulate hair growth through β-catenin and Shh expression in C57BL", 6 mice, 2015, pp.2015, 730139~
- Mirvish SS, "Kinetics of dimethylamine nitrosation in relation to nitrosamine carcinogenesis", Journal of the National Cancer Institute, 44, pp.633~639 (1970)
- 모정희, "황칠나무 잎 메탄올 추출물 및 분획물의 Tyrosinase 저해 및 Melanin 생성억제 활성", 아시안뷰티화장품학술지, 11(2), pp.275~280 (2013), 한국피부과학연구원
- Moon MO, "Taxonomic appraisal of Dendropanax morbifera Léveillé and D. trifidus (Thunb. ex Murray) Makino based on morphological characters", Korean Journal of Plant Taxonomy, 29, pp.231~248 (1999)
- Mosmann T, "Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays", Journal of Immunological Methods, 65, pp.55~63 (1983)
- 박찬성, "쑥과 솔잎의 항산화작용 및 아질산염 소거작용", 한국식품저장유통학회지, 9(2), pp.248~252 (2002), 한국식품저장유통학회
- Philpott MP, "Effects of insulin and insulin-like growth factors on cultured human hair follicles: IGF-I at physiologic concentrations is an important regulator of hair follicle growth in vitro", Journal of Investigative Dermatology, 102, pp.857~861 (1994)
- Piddock LJ, "Techniques used for the determination of antimicrobial resistance and sensitivity in bacteria", Journal of Applied Bacteriology, 68, pp.307~318 (1990)
- Randall VA, "Do androgens influence hair growth by altering the paracrine factors secreted by dermal papilla cells?", European Journal of Dermatology, 11, pp.315~320 (2001)
- Reuben A, "Antifungal susceptibility of 44 clinical isolates of Fusarium species determined by using a broth microdilution method", Antimicrobial Agents and Chemotherapy, 33, pp.1647~1649 (1989)
- Tsuboi R, "Growth factors and hair growth", Korean Journal of Investigative Dermatology, 4, pp.103~108 (1997)
- 염태현, "측백나무 열매 추출물의 항균활성", 한국약용작물학회지, 18(5), pp.315~322 (2010), 한국약용작물학회