감잎추출물의 항산화 및 항염증 효과
Anti-Oxidant and Anti-Inflammatory Effects of Diospyros kaki Thumb Leaves Extracts
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초록
Oxidative stress has been implicated in cutaneous damage in various inflammatory skin diseases, includingpsoriasis. Flavonoids have increased lately due to their broad pharmacological effect, explained by theirinhibition of certain enzymes and their antioxidant activity. Accordingly, to develop oxidant and inflammationcare cosmetic ingredients, the extracts Diospyros kaki leaves were selected. In this study, we investigatedeffect on cell viability and anti-oxidant activity of Diospyros kaki leaves extracts by using sulforhodamineB (SRB) assay and 1,1-diphenyl-2-picryl hydrazyl (DPPH) method. The anti-inflammatory activity ofDiospyros kaki leaves extracts on nitrite production and tumor necrosis factor-α (TNF-α) secretion weretested using nitric oxide (NO) colorimetric assay kit and enzyme-linked immunosorbent assay (ELISA) kit. Diospyros kaki leaves extracts showed the high cell viability over 100 % against mouse macrophage RAW264. 7 cell at concentration of 10, 50, 100 and 500 μg/mL. Those exhibited anti-oxidant activity with dosedependent manner at concentration of 10, 50, 100 and 500 μg/mL. Nitrite production and TNF-α secretionwere significantly decreased with dose dependant manner according to increment of treated concentrationwith Diospyros kaki leaves extracts (10, 50, 100 and 500 μg/mL) (p<.05). These results demonstrate thatDiospyros kaki leaves extract possessing anti-oxidant and anti-inflammatory effects may be applicable toheal skin damage.
Abstract
Oxidative stress has been implicated in cutaneous damage in various inflammatory skin diseases, includingpsoriasis. Flavonoids have increased lately due to their broad pharmacological effect, explained by theirinhibition of certain enzymes and their antioxidant activity. Accordingly, to develop oxidant and inflammationcare cosmetic ingredients, the extracts Diospyros kaki leaves were selected. In this study, we investigatedeffect on cell viability and anti-oxidant activity of Diospyros kaki leaves extracts by using sulforhodamineB (SRB) assay and 1,1-diphenyl-2-picryl hydrazyl (DPPH) method. The anti-inflammatory activity ofDiospyros kaki leaves extracts on nitrite production and tumor necrosis factor-α (TNF-α) secretion weretested using nitric oxide (NO) colorimetric assay kit and enzyme-linked immunosorbent assay (ELISA) kit. Diospyros kaki leaves extracts showed the high cell viability over 100 % against mouse macrophage RAW264. 7 cell at concentration of 10, 50, 100 and 500 μg/mL. Those exhibited anti-oxidant activity with dosedependent manner at concentration of 10, 50, 100 and 500 μg/mL. Nitrite production and TNF-α secretionwere significantly decreased with dose dependant manner according to increment of treated concentrationwith Diospyros kaki leaves extracts (10, 50, 100 and 500 μg/mL) (p<.05). These results demonstrate thatDiospyros kaki leaves extract possessing anti-oxidant and anti-inflammatory effects may be applicable toheal skin damage.
참고문헌 (28)
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