人蔘養榮湯이 비소세포 폐암세포 이식 마우스에서 항암, 면역활성 및 악액질 억제에 미치는 영향
Anticancer and Related Immunomodulatory and Anticachexic Effects of Insamyangyoung-tang Extracts on Non Small Cell Lung Carcinoma, NCI-H520, Xenograft Mice
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초록
The object of this study was to observe anticancer and related immunomodulatory and anticachexic effects of Insamyangyoung-tang aqueous extracts (ISYYTe) on non‐small cell lung carcinoma (squamous epithelial carcinoma), NCI-H520, xenograft Balb/c nu-nu nude mice. Changes on the tumor volume and weights, lymphatic organ(spleen and popliteal lymph node), serum interferon (IFN)-γ levels, splenocytes and peritoneal macrophage activities (NK cell activity), splenic tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-10 contents, splenic T-lymphocyte subsets (CD3+, CD4+ and CD8+) and TNF-α+ cells were observed with tumor mass and lymphatic organ histopathology to detect anticancer and immunomodulatory effects. In addition, changes on the body weights, epididymal fat weights and serum IL-6 levels were also detected with the thicknesses of deposited cervical brown adipose tissue and their mean diameters to monitor the tumor‐related anticachexic effects. The results obtained in this study suggest that over 50 ㎎/㎏ of ISYYTe showed favorable anticancer effects on the NCI-H520 cell xenograft with immunomodulatory and anticachexic effects. However, detail mechanism studies should be conducted in future with the screening of the biological active compounds in this herb.
Abstract
The object of this study was to observe anticancer and related immunomodulatory and anticachexic effects of Insamyangyoung-tang aqueous extracts (ISYYTe) on non‐small cell lung carcinoma (squamous epithelial carcinoma), NCI-H520, xenograft Balb/c nu-nu nude mice. Changes on the tumor volume and weights, lymphatic organ(spleen and popliteal lymph node), serum interferon (IFN)-γ levels, splenocytes and peritoneal macrophage activities (NK cell activity), splenic tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-10 contents, splenic T-lymphocyte subsets (CD3+, CD4+ and CD8+) and TNF-α+ cells were observed with tumor mass and lymphatic organ histopathology to detect anticancer and immunomodulatory effects. In addition, changes on the body weights, epididymal fat weights and serum IL-6 levels were also detected with the thicknesses of deposited cervical brown adipose tissue and their mean diameters to monitor the tumor‐related anticachexic effects. The results obtained in this study suggest that over 50 ㎎/㎏ of ISYYTe showed favorable anticancer effects on the NCI-H520 cell xenograft with immunomodulatory and anticachexic effects. However, detail mechanism studies should be conducted in future with the screening of the biological active compounds in this herb.
참고문헌 (63)
- "2007 annualreporton the cause of death statistics/("[URL]
- Kim, Y. C., "National survey of lung cancer in Korea, 2005", J. Lung Cancer, 6, pp.67~73 (2005)
- 한용철, "임상호흡기학", pp.137~ (1995), 일조각
- Nelson, K. A., "The cancer anorexia-cachexia syndrome", J of Clinical Oncology, 12(1), pp.213~225 (1994)
- 김은경, "입원한 암환자의 영양상태연구", 대한간호학회지, 29(2), pp.271~280 (1999)
- 翁家武, "癌症惡病質的中醫藥治療研究进展", 浙江中西醫結合雜誌, 19(2), pp.123~125 (2009)
- Yang, C. H., "Gemcitabine and cisplatinina multimodality treatment for locally advanced non-small cell lung cancer", Br J Cancer, 86, pp.190~195 (2002)
- Scarpati, M. D., "Gemcitabine and small cell lung cancer", Lung Cancer, 34, pp.313~314 (2001)
- Worden, F. P., "Therapeutic advances in small cell lung cancer", Expert Opin Investig Drugs, 9, pp.565~579 (2000)
- Massarelli, E., "Use of novel second-line targeted therapies in non-small cell lung cancer", Semin Oncol, 33, pp.9~16 (2006)
- Steinke, K., "Lung tumors", Recent Results Cancer Res, 167, pp.107~122 (2006)
- 이형범, "송이버섯과 동충하초 균사체를 혼합 배양한 한방추출물의 발효에 의한 생리활성", 대한본초학회지, 23(1), pp.1~8 (2008), 대한본초학회
- 함영안, "잔대 추출물들의 항돌연변이 및 항종양 효과", 한국식품영양과학회지, 38(1), pp.25~31 (2009), 한국식품영양과학회
- 유미희, "생대추(풋대추) 과육 및 씨 추출물의 항산화 효과 및 항산화 성분", 한국식품과학회지, 38(1), pp.128~134 (2006), 한국식품과학회
- 은재순, "인삼양영탕이 생쥐의 특이적 면역반응에 미치는 영향", 동의생리병리학회지, 16(4), pp.18~733 (2002), 대한동의병리학회
- 하지용, "人蔘養榮湯이 항암 및 면역조절작용에 미치는 영향", 동의병리학회지, 12, pp.60~71 (1998)
- Institute of Laboratory Animal Resourcis (Commission on Life Science, National Research Council, USA)., "Guide for the care and use of laboratory animals", pp.1~118 (1996), National Academic press
- Itoh, H., "Inhibitory action of a (1-->6)-beta-D-glucan-protein complex (FIII-2-b) isolated from Agaricus blazei Murill ("himematsutake") on Meth A fibrosarcoma‐bearing mice and its antitumor mechanism", Jpn J Pharmacol, 66, pp.265~271 (1994)
- Nishimura, G., "An antioxidant, probucol, induces anti‐angiogenesis and apoptosis in athymic nude mouse xenografted human head and neck squamous carcinoma cells", Jpn J Cancer Res, 90, pp.1224~1230 (1999)
- Liu, L., "Clinical and experimental study on regional administration of phosphorus 32 glass microspheres in treating hepatic carcinoma", World J Gastroenterol, 5, pp.492~505 (1999)
- Iizuka, N., "Anticachectic effects of Coptidis rhizoma, an anti‐inflammatory herb, on esophageal cancer cells that produce interleukin 6", Cancer Lett, 158, pp.35~41 (2000)
- Clark, B. D., "Detection of interleukin 1 alpha and 1 beta in rabbit tissues during endotoxemia using sensitive radioimmunoassays", J Appl Physiol, 71, pp.2412~2418 (1991)
- Hotchkiss, R. S., "Calcium antagonists decrease plasma and tissue concentrations of tumor necrosis factor alpha, interleukin‐1 beta, and interleukin‐1 alpha in a mouse model of endotoxin", Shock, 3, pp.337~342 (1995)
- Raffaghello, L., "Anti GD2 monoclonal antibody immunotherapy : a promising strategy in the prevention of neuroblastoma relapse", Cancer Lett, 197, pp.205~209 (2003)
- 대한병리학회 대구경북지부학회, "간추린 병리학", pp.191~217 (2008), 정문각
- 오인재, "비소세포 폐암 세포주에 대한 항암약제와 방사선 병합치료 방법의 개발", 박사, 의학, pp.2008
- Korea National Stastical Office, "Death rate stastistics of Korea, 2005" (2007), Korea National Stastical Office
- Scientific Committee of Korean Academy of Tuberculosis and Respiratory Diseases, "The national survey of lung cancer in Korea", Tuberc Respir Dis, 46, pp.455~465 (1999)
- Clark, J. C., "Development of chondrosarcoma animal models for assessment of adjuvant therapy", ANZ J Surg, 79, pp.327~336 (2009)
- Samira, S., "Tumor necrosis factor promotes human T‐cell development in nonobese diabetic", severe combined immunodeficient mice, 2004, pp.22, 1085~1100
- Grant, M., "Alterations in nutrition, In Cancer Nursing, a comprehensive textbook" (1996), london Saunders
- Kim, Y. O., "Association of Nutritional Status with Clinical Outcome of Stomach Cancer Patients", J. Korean Soc Food Sci Nutr, 29(6), pp.1185~1189 (2000)
- Burtis, G., "Applied nutrition and diet therapy", pp.484~487 (1988), saunder Company
- Rivadeneria, . DE., "Nutritional support of the cancer patients", CA Cancer J Clin, 48, pp.69~80 (1998)
- Shils, M. E., "Modern nutrition in health and disease", pp.1319~1326 (1994), Lea&Feriger
- McWhirter, J. P., "Incidence and recognition of malnutrition in hospital", BMJ, 308, pp.945~948 (1994)
- Kim, E. K., "Nutritional Status of Cancer Patients upon Admission", J. Korean Nurses Association, 29(2), pp.271~280 (1999)
- 송광규, "암성 악액질(cancer cachexia)의 한약치료에 대한 고찰 -2000~2009년까지 중의논문을 중심으로-", 한국한의학연구원논문집, 16(2), pp.181~191 (2010), 한국한의학연구원
- 申載鏞, "方藥合編解說", pp.42~ (1988), 成輔社
- 鞠潤範, "李棣熙", pp.294~ (2008), 永林社
- 許俊, "國譯增補 東醫寶鑑", pp.671~ (1992), 南山堂
- Hanada, M., "Amrubicin, a novel 9‐aminoanthracycline, enhances the antitumor activity of chemotherapeutic agents against human cancer cells in vitro and in vivo", Cancer Sci, 98, pp.447~454 (2007)
- Okabe, T., "Addition of S-1 to the epidermal growth factor receptor inhibitor gefitinib overcomes gefitinib resistance in non‐small cell lung cancer cell lines with MET amplification", Clin Cancer Res, 15, pp.907~913 (2009)
- Natsume, T., "Combination effect of TZT-1027(Soblidotin)with other anticancer drugs", Anticancer Res, 26, pp.1145~1151 (2006)
- Foster, P. A, "Steroid metabolism in breast cancer", Minerva Endocrinol, 33, pp.27~37 (2008)
- Isaacs, A, "Lymphokines and Cytokines. In: Immunology an introduction", pp.155~169 (1995), Saunders
- Unanue, E. R, "The Mononuclear‐Phagocytic Sytem. In: Immunology an introduction", pp.61~74 (1995), Saunders
- Sogn, J. A, "Tumor immunology : the glass is half full", Immunity, 9, pp.757~763 (1998)
- Finke, J., "Where have all the T cells gone? Mechanisms of immune evasion by tumors", Immunol Today, 20, pp.158~160 (1999)
- Pawelec, G., "Escape mechanisms in tumor immunity : a year 2000 update", Crit Rev Oncog, 11, pp.97~133 (2000)
- Jaffe, M. L., "Mechanisms of tumor induced immunosuppression : evidence for contact dependent T cell suppression by monocytes", Mol Med, 2, pp.692~701 (1996)
- Wang, R. F, "Immune suppression by tumor‐specific CD4+ regulatory T‐cells in cancer", Semin Cancer Biol, 16, pp.73~79 (2006)
- Hsieh, C. L., "Tumor‐induced immunosuppression : a barrier to immunotherapy of large tumors by cytokine‐secreting tumor vaccine", Hum Gene Ther, 11, pp.681~692 (2000)
- Riccobon, A., "Immunosuppression in renal cancer : differential expression of signal transduction molecules in tumor‐infiltrating, near‐tumor tissue, and peripheral blood lymphocytes", Cancer Invest, 22, pp.871~877 (2004)
- Ha, E. S., "Anti‐metastatic activity of glycoprotein fractionated from Acanthpanax senticosus, involvement of NK‐cell and macrophage activation", Arch Pharm Res, 27, pp.217~224 (2004)
- 유경미, "In vitro 상에서 유자(Citrus junos SIEB ex TANAKA) 용매 추출물의 암세포 억제효과 및 항산화성", 한국식품과학회지, 36(2), pp.339~344 (2004), 한국식품과학회
- Schimmel, K. J., "Cardiotoxicity of cytotoxic drugs", Cancer Treat Re, 30, pp.181~191 (2004)
- Dalzell, J. R., "The spectrum of 5-fluorouracil cardiotoxicity", Anticancer Drugs, 20, pp.79~80 (2009)
- Strassmann, G., "Evidence for the involvement of interleukin 6 in experimental cancer cachexia", J Clin Invest, 89, pp.1681~1684 (1992)
- Fujita, J., "Anti‐interleukin‐6 receptor antibody prevents muscle atrophy in colon‐26 adenocarcinoma bearing mice with modulation of lysosomal and ATP ubiquitin‐dependent proteolytic pathways", Int J Cancer, 68, pp.637~643 (1996)
- Kurebayashi, J., "Medroxyprogesterone acetate inhibits interleukin 6 secretion from KPL‐4 human breast cancer cells both in vitro and in vivo : a possible mechanism of the anticachectic effect", Br J Cancer, 79, pp.631~636 (1999)
- Oka, M., "Relationship between serum levels of interleukin 6, various disease parameters and malnutrition in patients with esophageal squamous cell carcinoma", Cancer Res, 56, pp.2776~2780 (1996)
- Wang, L. S., "Expression and up‐regulation of interleukin‐6 in oesophageal carcinoma cells by n‐sodium butyrate", Br J. Cancer, 80, pp.1617~1622 (1999)
