健脾補腎抗癌湯 및 健脾補腎抗癌湯加味方이 고형암 및 면역세포에 미치는 영향
Effects of Gunbibosinhangam-tang and Gunbibosinhangam-tang-gamibang on Solid Tumor and Immune Cells in Mice
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This study was carried out to investigate the anti-cancer effects of Gunbibosinhangam-tang (GBHTⅠ) and Gunbibosinhangam-tang-gamibang (GBHTⅡ, GBHTⅢ) on solid tumor and immune cells. The animals were divided into 4 groups ; Control, no treatment. GBHTⅠ, treatment with GBHT itself. GBHTⅡ, treatment with GBHT increased the quantity of Hedyotis Diffusae twice. GBHTⅢ, treatment with GBHT increased the quantity of Hedyotis Diffusae four times. We investigated the effects of GBHT on proliferation of solid tumor cells (S-180), thymocytes, splenocytes in vitro in order to examine cytotoxicity for S-180 and immuno-stimulating activities. The experiments that is about solid tumor weight and survival rate in tumor bearing mice were performed also. As compared with the control group, treatments with GBHTⅡ and GBHTⅢ suppressed the proliferation of S-180 effectively. Treatments with all experimental groups accelerated the proliferation of thymocytes and splenocytes significantly. In addition, GBHTⅢ was significantly decreased on solid tumor weight and increased on survival rate in tumor bearing mice. Based upon these results, we suggest that GBHT and GBHT-gamibang have both anti-cancer effects for S-180 and immuno-stimulating activities for thymocytes and splenocytes. Therefore, we conclude that GBHT and Hedyotis Diffusae is useful to treat the patients with cancer.
Abstract
This study was carried out to investigate the anti-cancer effects of Gunbibosinhangam-tang (GBHTⅠ) and Gunbibosinhangam-tang-gamibang (GBHTⅡ, GBHTⅢ) on solid tumor and immune cells. The animals were divided into 4 groups ; Control, no treatment. GBHTⅠ, treatment with GBHT itself. GBHTⅡ, treatment with GBHT increased the quantity of Hedyotis Diffusae twice. GBHTⅢ, treatment with GBHT increased the quantity of Hedyotis Diffusae four times. We investigated the effects of GBHT on proliferation of solid tumor cells (S-180), thymocytes, splenocytes in vitro in order to examine cytotoxicity for S-180 and immuno-stimulating activities. The experiments that is about solid tumor weight and survival rate in tumor bearing mice were performed also. As compared with the control group, treatments with GBHTⅡ and GBHTⅢ suppressed the proliferation of S-180 effectively. Treatments with all experimental groups accelerated the proliferation of thymocytes and splenocytes significantly. In addition, GBHTⅢ was significantly decreased on solid tumor weight and increased on survival rate in tumor bearing mice. Based upon these results, we suggest that GBHT and GBHT-gamibang have both anti-cancer effects for S-180 and immuno-stimulating activities for thymocytes and splenocytes. Therefore, we conclude that GBHT and Hedyotis Diffusae is useful to treat the patients with cancer.
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