본문 바로가기
KERD
논문KCI 등재

十六味流氣飮의 免疫活性에 의한 乳房癌 세포 成長抑制效果

Shipyeukmiyeugi-eum Extracts Suppressed Tumor Growth through Immunomodulatory Effects on MCF-7

김동철|박영선|정연철
대한한방부인과학회 (The Society Of Oriental Obstetrics & Gynecology)|2012.08|대한한방부인과학회지 (The Journal of Oriental Obstetrics ＆ Gynecology)|제25권 제3호|pp. 40-60|DOI http://dx.doi.org/

2

조회

0

다운로드

5

인용

0

참고문헌

키워드

TamoxifenBreast cancerShipyeukmiyeugi-eumAnticachexiaCytotoxicImmunomodulatory effect
주제분류한의학

초록

Objectives: The object of this study was to observe antitumor, anticachexia and immunomodulatory effects of Shipyeukmiyeugi-eum(SYM) on human breast cancer cell, MCF-7, xenograft Balb/c nu-nu nude mice. Methods: Three different dosages of SYM-125, 250 and 500 ㎎/㎏ were orally administered once a day for 28 days from 11 days after tumor cell inoculation,and the changes on the body weights, tumor volume and weights, weights of spleen and popliteal lymph node and epididymal fat, serum IL-6 and IFN-γ levels, NK cell and peritoneal macrophage activities, splenic TNF-α, IL-1β and IL-10 contents were observed. In addition, histopathological observations of apoptotic cell, spleen,popliteal lymph node and cervical brown adipose were also detected. The results were compared with a potent cytotoxic estrogen receptor antagonist, Tamoxifen 20 ㎎/㎏treated mice. Results: Tumor volumes and weights were decreased without cytotoxic effects on the both MCF-7 and MCF-10A cells as results of all three different dosages of SYM treatment. And weights of body, spleen, popliteal lymph node, epididymal fat, serum IFN-γ, NK cell, peritoneal macrophage activities, splenic TNF-α, IL-1βand IL-10 contents were increased with decrease of serum IL-6. At histopathological observations, apoptotic tumor cells, spleen, popliteal lymph node and cervical brown adipose tissue were increased. That means tumor-related immunosuppress and cachexia were markedly inhibited by SYM treatment as compared with tumor-bearing mice. On the other hand, Tamoxifen showed marked cytotoxic effects against MCF-7 and MCF-10A, decreases of tumor volume and weights, and increases of apoptotic tumor cells and related decreases of tumor cell volumes, but tamoxifen markedly deteriorated the tumor-related immune-suppress and cachexia. Conclusions: The results obtained in this study suggest that SYM showed favorable anticancer effects and anticachexic effects on the MCF-7 cell xenograft through immunomodulatory effects. SYM did not induce any cytotoxic effects against both normal and cancer cells.

Abstract

Objectives: The object of this study was to observe antitumor, anticachexia and immunomodulatory effects of Shipyeukmiyeugi-eum(SYM) on human breast cancer cell, MCF-7, xenograft Balb/c nu-nu nude mice. Methods: Three different dosages of SYM-125, 250 and 500 ㎎/㎏ were orally administered once a day for 28 days from 11 days after tumor cell inoculation,and the changes on the body weights, tumor volume and weights, weights of spleen and popliteal lymph node and epididymal fat, serum IL-6 and IFN-γ levels, NK cell and peritoneal macrophage activities, splenic TNF-α, IL-1β and IL-10 contents were observed. In addition, histopathological observations of apoptotic cell, spleen,popliteal lymph node and cervical brown adipose were also detected. The results were compared with a potent cytotoxic estrogen receptor antagonist, Tamoxifen 20 ㎎/㎏treated mice. Results: Tumor volumes and weights were decreased without cytotoxic effects on the both MCF-7 and MCF-10A cells as results of all three different dosages of SYM treatment. And weights of body, spleen, popliteal lymph node, epididymal fat, serum IFN-γ, NK cell, peritoneal macrophage activities, splenic TNF-α, IL-1βand IL-10 contents were increased with decrease of serum IL-6. At histopathological observations, apoptotic tumor cells, spleen, popliteal lymph node and cervical brown adipose tissue were increased. That means tumor-related immunosuppress and cachexia were markedly inhibited by SYM treatment as compared with tumor-bearing mice. On the other hand, Tamoxifen showed marked cytotoxic effects against MCF-7 and MCF-10A, decreases of tumor volume and weights, and increases of apoptotic tumor cells and related decreases of tumor cell volumes, but tamoxifen markedly deteriorated the tumor-related immune-suppress and cachexia. Conclusions: The results obtained in this study suggest that SYM showed favorable anticancer effects and anticachexic effects on the MCF-7 cell xenograft through immunomodulatory effects. SYM did not induce any cytotoxic effects against both normal and cancer cells.

참고문헌 (44)

  1. "국가암등록사업 연례 보고서" (2009), 보건복지가족부[URL]
  2. Oka M, "Relationship between serum levels of Il-6, various disease parameters and malnutrition in patients with esophageal squamous cell carcinoma", Cancer Res, 56(12), pp.2776~2780 (1996)
  3. Ha ES, "Anti-metastatic activity of glycoprotein fractionated from Acanthpanax senticosus, involvement of NK-cell and macrophage activation", Arch Pharm Res, 27(2), pp.217~224 (2004)
  4. 유경미, "In vitro 상에서 유자(Citrus junos SIEB ex TANAKA) 용매 추출물의 암세포 억제효과 및 항산화성", 한국식품과학회지, 36(2), pp.339~344 (2004), 한국식품과학회
  5. 陳夢雷, "古今圖書集成 醫部全錄", pp.54~135 (1983), 人民衛生出版社
  6. Detre S, "Comparison of the selective estrogen receptor modulator arzoxifene(LY353381)with tamoxifen on tumor growth and biomarker expression in an MCF-7 human breast cancer xenograft model", Cancer Res, 63(19), pp.6516~6522 (2003)
  7. Nishimura G, "An antioxidant, probucol, induces anti-angiogenesis and apoptosis in athymic nude mouse xenografted human head and neck squamous carcinoma cells", Jpn J Cancer Res, 90(11), pp.1224~1230 (1999)
  8. Iizuka N, "Anticachectic effects of Coptidis rhizoma, an anti-inflammatory herb, on esophageal cancer cells that produce interleukin 6", Cancer Lett, 158(1), pp.35~41 (2000)
  9. Zarling JM, "Augmentation of natural killer cell activity by polyinosinic acid-polycytidylic acid and its nontoxic mismatched analogues", J Immunol, 124(4), pp.1852~1857 (1980)
  10. Clark BD, "Detection of interleukin 1 alpha and 1 beta in rabbit tissues during endotoxemia using sensitive radio immunoassays", J Appl Physiol, 71(6), pp.2412~2418 (1991)
  11. Hotchkiss RS, "Calcium antagonists decrease plasma and tissue concentrations of tumor necrosis factor-alpha, interleukin-1 beta, and interleukin-1 alpha in a mouse model of endotoxin", Shock, 3(5), pp.337~342 (1995)
  12. Liu L, "Clinical and experimental study on regional administration of phosphorus 32 glass microspheres in treating hepatic carcinoma", World J Gastroenterol, 5(6), pp.492~505 (1999)
  13. "국가 암정보센터"[URL]
  14. 翁家武, "癌症惡病質的中醫藥治療硏究進展", 中西醫結合雜誌, 19(2), pp.271~280 (2009)
  15. 許浚, "東醫寶鑑 外形偏", pp.407~414 (1992), 대성문화사
  16. 김정진, "유방종괴에 관한 문헌적 고찰", 대한한방부인과학회지, 11(2), pp.29~43 (1998)
  17. Makino T, "Suppressive effects of Perilla frutescens on IgA nephropathy in HIGA mice", Nephrol Dial Transplant, 18(3), pp.484~490 (2003)
  18. Oh HA, "Effect of Perilla frutescens var. acuta Kudo and rosmarinic acid on allergic inflammatory reactions", Experimental Biology and Medicine, 236(1), pp.99~106 (2011), Society for Experimental Biology and Medicine
  19. Jang HI, "Wild Panax Ginseng (Panax ginseng C.A. Meyer) Protects Against Methotrexate-Induced Cell Regression by Enhancing the Immune Response in RAW 264.7 Macrophages", AMERICAN JOURNAL OF CHINESE MEDICINE, 38(5), pp.949~960 (2010), WORLD SCIENTIFIC PUBL CO PTE LTD
  20. Weihua Ni, "Antitumor Activities and Immunomodulatory Effects of Ginseng Neutral Polysaccharides in Combination with 5-Fluorouracil", Journal of Medicinal Food, 13(2), pp.270~277 (2010), 한국식품영양과학회
  21. Stacia Clement-Kruzel, "Immune Modulation of Macrophage Pro-Inflammatory Response by Goldenseal and Astragalus Extracts", Journal of Medicinal Food, 11(3), pp.493~498 (2008), 한국식품영양과학회
  22. Han SB, "Characteristic immune stimulation by angelan isolated from Angelica gigas Nakai", Immunopharmacology, 40(1), pp.39~48 (1998)
  23. 임강현, "백지의 사람비만세포 사이토카인 및 케모카인 발현 양상", 대한본초학회지, 22(1), pp.81~88 (2007), 대한본초학회
  24. Luo Y, "Total alkaloids from Radix Linderae prevent the production of inflammatory mediators in lipopoly saccharide-stimulated RAW 264.7 cells by suppressing NF-kappa B and MAPKs activation", Cytokine, 46(1), pp.104~110 (2009)
  25. Jeong JB, "Antioxidant activity in essential oils of Cnidium officinale makino and Ligusticum chuanxiong Hort and their inhibitory effects on DNA damage and apoptosis induced by ultraviolet B in mammalian cell", Cancer Epidemiol, 33(1), pp.41~46 (2009)
  26. 정진부, "Protective effect of the extracts from Cnidium officinale against oxidative damage induced by hydrogen peroxide via antioxidant effect", FOOD AND CHEMICAL TOXICOLOGY, 47(3), pp.525~529 (2009), PERGAMON-ELSEVIER SCIENCE LTD
  27. Kim NY, "Butanolides from Machilus thunbergii and their inhibitory activity on nitricoxide synthesis in activated macrophages", Phytother Res, 17(4), pp.372~375 (2003)
  28. Tai J, "Anti-proliferative and antioxidant activities of Saposhnikovia divaricata", Oncol Rep, 18(1), pp.227~234 (2007)
  29. Pithayanukul P, "Hepa toprotective potential of extracts from seeds of Areca catechu and nutgalls of Quercus infectoria", Molecules, 14(12), pp.4987~5000 (2009)
  30. 정연희, "십육미유기음(十六味流氣飮) 및 그 가미방이 갑상선 기능에 미치는 영향", 대한한의학회지, 9, pp.98~108 (1988)
  31. 李枝映, "十六味流氣飮煎湯液이 實驗動物에 미치는 影響", 원광한의학, 3, pp.149~165 (1993)
  32. 심상희, "십육미유기음의 항암 및 항암제 부작용에 미치는 영향", 석사, pp.2003
  33. 洪淳昇, "洪家定診秘傳", pp.67~69 (1974), 醫藥社
  34. 朴南錯, "韓方治療大全", pp.125~349 (1979), 南山堂
  35. 朴炳昆, "漢方臨床 40年", pp.334~338 (1984), 大成文化社
  36. 李基淳, "漢方內科學", pp.300~372 (1982), 壽文社
  37. Yaacob NS, "Anticancer activity of a sub-fraction of dichloromethane extract of Strobilanthescrispus on human breast and prostate cancer cells in vitro", BMC Complement Altern Med, 5(10), pp.42~ (2010)
  38. 김정숙, "유용에 활용되는 가감유기음에 대한 실험적연구", 대한한방부인과학회지, 8(1), pp.115~130 (1995)
  39. Kurebayashi, J., "Medroxyproges terone acetate inhibits interleukin 6 secretion from KPL-4 human breast cancer cells both in vitro and in vivo: a possible mechanism of the anticachectic effect", Br J Cancer, 79(3-4), pp.631~636 (1999)
  40. Isaacs A, "Lymphokines and Cytokines, In Immunology an introduction (Tizard IR editor). 4th edition", pp.155~169 (1995), Saunders
  41. 서울대학교의과대학, "종양학", pp.229~ (1998), 서울대학교출판부
  42. Ripoll V.M., "Microphthlmia transcription factor regulates the expression of the novel osteoclast factor GPNMB", Gene, 413(1-2), pp.32~41 (2008)
  43. Unanue ER, "The Mononuclear- Phagocytic Sytem, In Immunology an introduction (Tizard IR editor). 4th edition", pp.61~74 (1995), Saunders
  44. Joffroy CM, "Antiestrogens induce transforming growth factor beta-mediated immune suppression in breast cancer", Cancer Res, 70(4), pp.1314~1322 (2010)

관련 논문AI추천

Antitumor Effects of Cistanchis Herba Aqueous Extracts on MCF-7 Human Breast Cancer Cell-Xenograft Athymic Nude Mice, through Potent Immunomodulatory Activities김동철, 황현지대한한방부인과학회 · 대한한방부인과학회지2024 · 제37권 제1호人蔘養榮湯이 비소세포 폐암세포 이식 마우스에서 항암, 면역활성 및 악액질 억제에 미치는 영향김종대, 박미연, 송광규, 최해윤한의병리학회 · 동의생리병리학회지2013 · 제27권 제1호淸肝解鬱湯과 十六味流氣飮의 유방암에 대한 항암, 항염 효능 연구류효경, 정민재, 조성희대한한방부인과학회 · 대한한방부인과학회지2022 · 제35권 제3호歸脾湯이 비소세포 폐암세포(NCI-H520) 이식 마우스에서 항암 및 면역 활성에 미치는 영향김종대, 손지영, 최해윤대한한방내과학회 · 대한한방내과학회지2012 · 제33권 제4호桑白皮의 선천면역 활성화에 의한 항암 효과이경섭, 이진무, 이창훈, 장준복, 정재혁, 조정훈대한한방부인과학회 · 대한한방부인과학회지2009 · 제22권 제1호增損五積丸 脾積方이 생쥐의 복강암에 미치는 영향김종한, 박수연, 이유진, 최정화대한한방안이비인후피부과학회 · 한방안이비인후피부과학회지2010 · 제23권 제1호

이 간행물의 다른 논문 (12건)

모과(木瓜) 물추출물의 항염증 효능에 관한 실험적 연구임은미, 김윤상, 류한우pp. 1-15동충하초가 파골세포의 분화와 유전자 발현에 미치는 영향최경희, 황귀서, 유정은, 유동열pp. 16-26透膿散 및 瓜蔞牛蒡湯의 Staphylococcus aureus에 대한 In Vitro 항균력 평가장세란, 박영선, 김동철pp. 27-39肉桂 추출물이 Rat fetus 두개골로부터 분리한 조골세포에 미치는 영향김덕구, 유동열pp. 61-70갈근 추출물이 난소 적출로 비만이 유발된 Rat의 체중 변화와 유전자 발현에 미치는 영향유정은, 정은혜, 신희종, 유동열pp. 71-84
원문 보기