삼지구엽초의 멜라닌합성 촉진과 작용기전
Melanogenic Effect and the Mechanism of Epimedium koreanum Nakai
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In this study, ethanol extract of Epimedium koreanum Nakai(EEKN) enhanced melanogenesis by inducing expression of tyrosinase and tyrosinase-related protein-1 (TRP-1). But EEKN did not increase the protein expression of tyrosinase-related protein 2 (TRP-2). Moreover, EEKN enhanced tyrosinase activity and melanin contents of B16F10 cells. EEKN raised the expression of CREB phosphorylation and microphthalmia-associated transcription factor (MITF) as a key transcription factor for tyrosinase expression regulating melanogenesis. And PKC inhibitor H89 supressed that EEKN induced tyrosinase activity, melanin contents, and expression of tyrosinase, TRP-1. These results suggest that melanogenesis-promoting effect of EEKN was correlated with regulation of tyrosinase and TRP-1 protein through cAMP/PKC pathway.
Abstract
In this study, ethanol extract of Epimedium koreanum Nakai(EEKN) enhanced melanogenesis by inducing expression of tyrosinase and tyrosinase-related protein-1 (TRP-1). But EEKN did not increase the protein expression of tyrosinase-related protein 2 (TRP-2). Moreover, EEKN enhanced tyrosinase activity and melanin contents of B16F10 cells. EEKN raised the expression of CREB phosphorylation and microphthalmia-associated transcription factor (MITF) as a key transcription factor for tyrosinase expression regulating melanogenesis. And PKC inhibitor H89 supressed that EEKN induced tyrosinase activity, melanin contents, and expression of tyrosinase, TRP-1. These results suggest that melanogenesis-promoting effect of EEKN was correlated with regulation of tyrosinase and TRP-1 protein through cAMP/PKC pathway.
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