玫瑰花 에탄올추출물이 α-MSH로 유도된 과색소 형성 억제와 작용기전 연구
Inhibitory Effect of the Ethanol Extract of Rosae rugosae Flos on the Hyperpigmentation and its Action Mechanism Induced by α-MSH
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Objective : This study investigated the inhibitory mechanism of the hypopigmentating effects on ethanol extractof Rosae rugosae Flos (ERR) that has not yet been examined. Methods : We analyzed the anti-melanogenic effects of ethanol extracts from Rosae rugosae Flos by tyrosinaseactivity, melanin contents. We also examined protein expression levels of tyrosinase, TRP-1, TRP-2, MITF and ERKby western blot analysis in melanoma cells. Results : In this investigation, ERR effectively reduced α-MSH-stimulated melanin synthesis by suppressingexpression of tyrosinase and tyrosinase-related protein-1 (TRP-1). On the other hand, the expression oftyrosinase-related protein-2 (TRP-2) were not affected by treatment with ERR. ERR inhibited the expression ofmicrophthalmia-associated transcription factor (MITF) as a key transcription factor for tyrosinase expressionregulating melanogenesis. The upstream signaling pathway including cAMP response element-binding protein(CREB) and MAPKs were also inhibited by ERR. Pretreatment with PD98059, ERK inhibitor, attenuated theinhibitory effect of ERR on α-MSH-induced tyrosinase activity. Conclusions : Our study suggested that the anti-melanogenic activity of ERR is correlated with the suppressionof tyrosinase gene through CREB/MITF/ERK pathway.
Abstract
Objective : This study investigated the inhibitory mechanism of the hypopigmentating effects on ethanol extractof Rosae rugosae Flos (ERR) that has not yet been examined. Methods : We analyzed the anti-melanogenic effects of ethanol extracts from Rosae rugosae Flos by tyrosinaseactivity, melanin contents. We also examined protein expression levels of tyrosinase, TRP-1, TRP-2, MITF and ERKby western blot analysis in melanoma cells. Results : In this investigation, ERR effectively reduced α-MSH-stimulated melanin synthesis by suppressingexpression of tyrosinase and tyrosinase-related protein-1 (TRP-1). On the other hand, the expression oftyrosinase-related protein-2 (TRP-2) were not affected by treatment with ERR. ERR inhibited the expression ofmicrophthalmia-associated transcription factor (MITF) as a key transcription factor for tyrosinase expressionregulating melanogenesis. The upstream signaling pathway including cAMP response element-binding protein(CREB) and MAPKs were also inhibited by ERR. Pretreatment with PD98059, ERK inhibitor, attenuated theinhibitory effect of ERR on α-MSH-induced tyrosinase activity. Conclusions : Our study suggested that the anti-melanogenic activity of ERR is correlated with the suppressionof tyrosinase gene through CREB/MITF/ERK pathway.
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