본문 바로가기
KERD
논문KCI 등재

한련초 에탄올 추출물의 멜라닌 합성 경로에 대한 연구

A Study of Melanin Synthesis Pathway of the Ethanol extract of E clip t a p r o s t r a t a In Vitro Study

2

조회

0

다운로드

1

인용

0

참고문헌

키워드

주제분류한의학

초록

Objectives : This Study was conducted to investigate the melanin synthesis effect of E cli p t a p r o s t r a t a and to determine the relationship between melanin synthesis effect of Eclipta prostrata and cAMP/PKA melanin synthesis pathway. Methods : We measured melanin contents, tyrosinase activity, expression of TRPs, p-CREB in B16F10 cells cultured with E cli p t a p r o s t r a t a ethanol extracts(EEP). And after treatment with H89 and dibutyryl cAMP, which inhibit or promote the activation of PKA, we observed changes in melanin synthesis and tyrosinase activity stimulated by EEP. Results : EEP increased melanin synthesis by promoting the expression of tyrosinase and TRP-1. It also promoted expression of p-CREB. H89 suppresses melanogenic effect and expression of tyrosinase, TRP-1 in B16F10 stimulated by EEP. Dibutyryl cAMP promotes melanogenic effect of EEP. Conclusions : The results of this study suggest that melanin synthesis effect of EEP is related to induction of tyrosinase and TRP-1 expression through the cAMP/PKA pathway.

Abstract

Objectives : This Study was conducted to investigate the melanin synthesis effect of E cli p t a p r o s t r a t a and to determine the relationship between melanin synthesis effect of Eclipta prostrata and cAMP/PKA melanin synthesis pathway. Methods : We measured melanin contents, tyrosinase activity, expression of TRPs, p-CREB in B16F10 cells cultured with E cli p t a p r o s t r a t a ethanol extracts(EEP). And after treatment with H89 and dibutyryl cAMP, which inhibit or promote the activation of PKA, we observed changes in melanin synthesis and tyrosinase activity stimulated by EEP. Results : EEP increased melanin synthesis by promoting the expression of tyrosinase and TRP-1. It also promoted expression of p-CREB. H89 suppresses melanogenic effect and expression of tyrosinase, TRP-1 in B16F10 stimulated by EEP. Dibutyryl cAMP promotes melanogenic effect of EEP. Conclusions : The results of this study suggest that melanin synthesis effect of EEP is related to induction of tyrosinase and TRP-1 expression through the cAMP/PKA pathway.

참고문헌 (42)

  1. Korean Dermatological Association, "Dermatology", pp.17~ (2008), RMGP
  2. Hill HZ, "Melanin;A two edged sword?", Pigment Cell Res, 10, pp.158~161 (1997)
  3. 전은경, "백반증 환자의 삶의 질 평가", 대한피부과학회지, 46(7), pp.874~880 (2008), 대한피부과학회
  4. 정승현, "백반증에서 미세분할 레이저의 색소침착 유도 효과: 예보", 대한피부과학회지, 47(5), pp.547~553 (2009), 대한피부과학회
  5. 강철호, "Melanogenesis-Promoting Effects of Sophora japonica Methanol Extract in Mean-a Cells", 대한미용학회지, 11(2), pp.89~96 (2015), 대한미용학회[DOI]
  6. Chun HJ, "Inhibitory effects of reticoic acid and melanization of B16 Melanoma cell by Epimuedium Koreanu Nakai and α-MSH", J Korean Chemical Society, 44(6), pp.533~540 (2000)
  7. 고진애, "In vitro에서 음양곽 열수추출물이 멜라닌 합성관련 효소 및 인자들에 미치는 영향", 대한미용학회지, 7(1), pp.45~52 (2011), 대한미용학회
  8. Chun HJ, "Effect of the Aqueous Extract of Epimedium Koreanum Nakai on Melanin Formation in B16 Mouse Melanoma Cell Line", J Traditional Korean Medicine, 10(2), pp.193~203 (2000)
  9. 이응지, "음양곽 추출물의 멜라닌 생성 촉진 효과", 대한화장품학회지, 35(4), pp.265~270 (2009), 대한화장품학회
  10. 이재순, "Promoting Effects of Soybean Methanol Extract on the Melanogenesis and Tyrosinase Activity", 대한미용학회지, 9(4), pp.8~14 (2013), 대한미용학회
  11. 홍성희, "Rhynchosia nulubilis and Rhynchosia volubilis Ethanol Extracts Upregulate the Expression of Genes Related to Melanogenesis in Melan-a Cells", 대한미용학회지, 12(2), pp.109~115 (2016), 대한미용학회[DOI]
  12. 조남철, "B16 Melanoma Cell에서 티로시나아제 유전자 발현에 황금(Scutellaria baicalensis) 추출물이 미치는 효과", 한국식품영양학회지, 23(1), pp.118~123 (2010), 한국식품영양학회
  13. 정재호, "補骨脂추출물이 B16 melanoma 세포주의 멜라닌 합성에 미치는 영향", 대한한의학회지, 26(3), pp.55~65 (2005), 대한한의학회
  14. 서형식, "補骨脂의 高梁酒, 燒酒 추출물이 B16 melanoma세포주의 멜라닌 합성에 미치는 영향", 한방안이비인후피부과학회지, 21(1), pp.96~103 (2008), 대한한방안이비인후피부과학회
  15. 최원형, "산수유 메탄올 추출물이 B16", F10 Melanoma 세포주의 멜라닌 생성에 미치는 영향, 2003, pp.34(1), 70~74[DOI]
  16. 양현옥, "산수유 물추출물이 B16", F10 melanoma세포주의 멜라닌 생성에 미치는 영향, 2002, pp.16(4), 29~822[DOI]
  17. Lee SD, "A Documentary Study on Herb, Dmgs Used for Vitiligo", J Oriental Medicine, 16(2), pp.44~61 (1995)
  18. Shin MK, "Clinical Traditional Herbalogy", pp.284~ (2006), YeongLim publishing
  19. Chang CA, "A Study on the Hair Dye Using the Extract of Hanryencho Depending on the Mordanting Methods", pp.1~34 (2006), Graduate school Catholic University of Daegu
  20. Huh J, "Donguibogam", pp.555~563 (2006), Shinwoo Publishing
  21. Mosmann T, "Rapid colorimetric assays for cellular growth and survival;application to proliferation and cytotoxicity assays", J Immunol Methods, 65(1), pp.55~63 (1983)
  22. Hosoi JE, "Regulation of melanin synthesis of B16 mouse melanoma cells by 1a,25-dehydroxyvitamin D3 and retinoic acid", Cancer Res, 45(4), pp.1474~1482 (1985)
  23. Martinez-Esparza M, "Mechanisms of melanogenesis inhibition by tumor necrosis factor-alpha in B16", F10 mouse melanoma cells, 1998, pp.255(1), 139~146
  24. Andrzej S, "Melanin Pigmentation in Mammalian Skin and Its Hormonal Regulation", Physiol Rev, 84, pp.1155~1228 (2004)
  25. Roser B, "Cyclic AMP a Key Messenger in the Regulation of Skin Pigmentation", Pigment Cell Res, 13, pp.60~69 (2000)
  26. Gordon PR, "Human Melanogenesis is Stimulated by Diacylglycerol", J Invest Dermatol, 93, pp.700~702 (1989)
  27. Christine RG, "Ultraviolet B Radiation Acts through the Nitric Oxide and cCMP Signaling Transduction Pathway to Stimulate Melanogenesis in Muman Melanocytes", J Biological Chemistry, 271(45), pp.28052~28056 (1996)
  28. Chan CF, "Fermented Broth in Tryosinase and Melanogenesis inhibition", Molecules, 19(9), pp.13122~13135 (2014)
  29. Korner A, "Activation of melanoma tyrosinase by a cyclic AMP-dependent protein kinase in a cell-free system", Nature, 267, pp.444~447 (1977)
  30. Pawelek J, "Melanoma cells resistant to inhibition of growth by melanocyte stimulating hormone", Proc Natl Acad Sci USA, 72, pp.951~955 (1975)
  31. Bertolotto C, "Different cis-acting elements are involved in the regulation of TRP1 and TRP2 promoter activities by cyclic AMP:pivotal role of M boxes(GTCATGTGCT) and of mirophthalmia", Mol Cell Biol, 18, pp.694~702 (1998)
  32. Takeda K, "SER298 of MITF, a mutation site in Waardenburg syndrome type 2, is a phosphorylation site with functional significance", Hum Mol Genet, 9(1), pp.125~132 (2000)
  33. Park HY, "Signaling pathways mediating melanogenesis", Cell Mol Biol, 45, pp.919~930 (1999)
  34. Lee SM, "An Experimental Study on the Effect of Serum Prothrombin Time by the Administration of Ecliptae herba Extraction in Mouse", Kyung Hee University Oriental Medical J, 5, pp.291~296 (1982)
  35. Mohammad S Rahman, "Antimicrobial activity and cytotoxicity of Eclipta prostrata", Oriental Pharmacy and Experimental Medicine, 8(1), pp.47~52 (2008), 한의학연구소
  36. 정진란, "한련초(Eclipta prostrata L. )를 이용한 모낭염 개선 효과", 아시안뷰티화장품학술지, 11(1), pp.167~171 (2013), 한국피부과학연구원
  37. 조희창, "旱蓮草 추출물의 항염증 효과", 한방안이비인후피부과학회지, 23(2), pp.125~138 (2010), 대한한방안이비인후피부과학회
  38. 문영희, "한련초로부터 정제한 다당체의 혈당강하 효과", 생약학회지, 34(3), pp.246~249 (2003), 한국생약학회
  39. Choi SW, "The Effects of Eclipta Prostrata in Insulin Resistance on Obese Mouse Model Induced by High Fat Diet", pp.1~48 (2014), Graduate school Dongshin University
  40. 박정현, "白鼠의 B16-F10 Melanoma에 대한 旱蓮草藥鍼의 抗癌 및 免疫增强效果", Journal of Acupuncture Research, 21(6), pp.63~84 (2004), 대한침구의학회
  41. Ha NR, "Antifibrotic Compounds from the Aerial Parts of Eclipta Prostrata in Liver Fibrosis", pp.1~92 (2007), Graduate school Seoul University
  42. 차수빈, "한련초의 멜라닌합성 촉진 효과", 동의생리병리학회지, 30(6), pp.432~438 (2016), 한의병리학회[DOI]

관련 논문AI추천

이 간행물의 다른 논문 (12건)