In vitro에서 음양곽 열수추출물이 멜라닌 합성관련 효소 및 인자들에 미치는 영향
The Effect of Epimedium koreanum Water Extract on the Enzymes and Factor Relevant to Melanin synthesis in Vitro Test
2
조회
0
다운로드
6
인용
0
참고문헌
키워드
초록
This study was conducted to evaluate the promoting effects of Epimedium koreanum water extract (EKWE) on the enzymes and factor relevant to melanin synthesis using enzyme activity, RT-PCR and Western blotting assays in melan-a cells. Intra-cellular tyrosinase activities of EKWE were higher as 3.9%, 9.2% (p<0.01), 13.0% (p<0.001), and 16.9%(p<0.001) at concentrations of 1.563, 3.125, 6.25, and 12.5 μg/mL, respectively, than the control group. Cell-extracted tyrosinase activities of EKWE were higher as 5.8%, 10.4% (p<0.01), 14.2% (p<0.01), and 24.1% (p<0.001) at concentrations of 1.563, 3.125, 6.25, and 12.5 μg/mL, respectively, than the control group. EKWE showed a promoting effect on tyrosinase activity. In Western blotting assay, EKWE significantly increased the protein expression of tyrosinase and TRP-2. In RT-PCR assay, EKWE significantly increased gene expression of tyrosinase, TRP-1 and MITF-M. In conclusion, EKWE has the promoting effect on the tyrosinase activity that is resulted from the up-regulation of its protein and gene expression.
Abstract
This study was conducted to evaluate the promoting effects of Epimedium koreanum water extract (EKWE) on the enzymes and factor relevant to melanin synthesis using enzyme activity, RT-PCR and Western blotting assays in melan-a cells. Intra-cellular tyrosinase activities of EKWE were higher as 3.9%, 9.2% (p<0.01), 13.0% (p<0.001), and 16.9%(p<0.001) at concentrations of 1.563, 3.125, 6.25, and 12.5 μg/mL, respectively, than the control group. Cell-extracted tyrosinase activities of EKWE were higher as 5.8%, 10.4% (p<0.01), 14.2% (p<0.01), and 24.1% (p<0.001) at concentrations of 1.563, 3.125, 6.25, and 12.5 μg/mL, respectively, than the control group. EKWE showed a promoting effect on tyrosinase activity. In Western blotting assay, EKWE significantly increased the protein expression of tyrosinase and TRP-2. In RT-PCR assay, EKWE significantly increased gene expression of tyrosinase, TRP-1 and MITF-M. In conclusion, EKWE has the promoting effect on the tyrosinase activity that is resulted from the up-regulation of its protein and gene expression.
참고문헌 (25)
- 고진애, "In vitro에서 음양곽 열수추출물의 항산화능 및 멜라닌 생성 촉진능", 대한미용학회지, 6(3), pp.279~286 (2010), 대한미용학회
- 백진옥, "백모증과 동반된 미만성 신경섬유종 1예", 대한피부과학회지, 45(8), pp.811~813 (2007), 대한피부과학회
- 이진옥, "染色 後 經時的인 毛髮의 形態變化와 染色 影響認識", 한국미용학회지, 14(2), pp.524~534 (2008), 한국미용학회
- 송희진, "백반증 병변의 백모에서 비활동성 멜라닌세포의 존재", 석사, pp.2009
- 천현자, "음양곽 물추출물 이 B16 mouse melanoma 세포의 멜라닌 생성에 미치는 영향", 약학회지, 44(5), pp.455~462 (2000)
- Botchkareva NV, "SCF", c-kit signaling is required for cyclic regeneration of the hair pigmentation unit, 2001, pp.15(3), 645~658
- Busca R, "Cyclic AMP: a key messenger in the regulation of skin pigmentation", Pigment Cell Research, 13(2), pp.60~69 (2000)
- Chung IM, "Screening of Korean medicinal food plants with antioxidant activity", Korean Journal of Medicinal Crop Science, 6, pp.311~322 (1998)
- Han SK, "Definition of vitiligo, in Vitiligo. 1st ed", pp.3~5 (2000), Blackwell Science
- Hunt G, "Cultured human melanocytes respond to MSH peptides and ACTH", Pigment Cell Research, 7(4), pp.217~221 (1994)
- Ito S, "Quantitative analysis of eumelanin and pheomelanin in humans, mice, and other animals: a comparative review", Pigment Cell Research, 16(5), pp.523~531 (2003)
- Songhee Jeon, "Lotus (Nelumbo nuficera) flower essential oil increased melanogenesis in normal human melanocytes", Experimental and Molecular Medicine, 41(7), pp.517~524 (2009), 생화학분자생물학회
- Lee JM, "Antioxidant activity of the aerial part of Epimedium koreanum Nakai", Journal of Korean Food Science and Nutrition, 26, pp.732~736 (2000)
- Moriyama M, "Notch signaling via Hes1 transcription factor maintains survival of melanoblasts and melanocyte stem cells", Journal of Cell Biology, 173(3), pp.333~339 (2006)
- Njoo MD, "Vitiligo: pathogenesis and treatment", American Journal of Clinical Dermatology, 2, pp.167~181 (2001)
- Ohguchi K, "Effects of sesquiterpene lactones on melanogenesis in mouse B16 melanoma cells", Biological and Pharmaceutical Bulletin, 32(2), pp.308~310 (2009)
- Olivares C, "New insights into the active site structure and catalytic mechanism of tyrosinase and its related proteins", Pigment Cell Melanoma Research, 22(6), pp.750~760 (2009)
- Park HY, "MITF mediates cAMP induced protein kinase C-β expression in human melanocytes", Biochemical Journal, 359(3), pp.571~578 (2006)
- Schallreuter KU, "Regulation of melanogenesis-controversies and new concepts", Experimental Dermatology, 17(5), pp.395~404 (2008)
- Sharov A, "Changes in different melanocyte populations during hair follicle involution (catagen)", Journal of Investigative Dermatology, 125(6), pp.1259~1267 (2005)
- Shin KH, "Difference in components of Epimedium koreanum in compliance with seasons and places of collection", Korean Journal of Medicinal Crop Science, 4, pp.321~328 (1996)
- Wakamatsu K, "Usefulness of 4-amino-3-hydroxyphenylalanine as a specific marker of pheomelanin", Pigment Cell Research, 15, pp.225~232 (2002)
- Winder A, "The tyrosinase gene family: interactions of melanogenic proteins to regulate melanogenesis", Cellular and Molecular Biology Research, 40(7), pp.613~626 (1994)
- Yaar M, "Human melanocyte growth and differentiation: a decade of new date", Journal of Investigative Dermatology, 97(4), pp.611~617 (1991)
- Yang Z, "Effect of ionic and nonionic surfactants on the activity and stability of mushroom tyrosinase", Journal of Molecular Catalysis B: Enzymatic, 47(4), pp.79~85 (2007)