魚腥草의 항산화 효능 확인 및 모유두 세포의 5α-reductase 유전자 발현에 미치는 영향
Investigation of Antioxidant Activity of Houttuyniae Herba and its Effect on 5α-reductase Gene Expression in Dermal Papilla Cells
4
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
Houttuyniae Herba is widely used as a cosmetic for enhancing hair growth, and study on promoting mouse hair growth has also been reported. However, studies on the effects of the Houttuyniae Herba on dermal papilla (DP) cells, which play an important role in hair growth, are not well known. For this reason, we studied the effect of Houttuyniae Herba on DP cells. The strong antioxidant activity of Houttuyniae Herba was confirmed by ABTS assay. In the MTS assay, cell viability was reduced to 94.5% in DP cells by treatment of 2 mg/ml concentration of Houttuyniae Herb and cytotoxicity was not observed at 1 mg/ml concentration. The mRNA expression levels of Bone morphogenetic pretein (BMP6), fibroblast growth factor 7 (FGF7), FGF10, and β-galactosidase genes, which are involved in hair growth cycle and hair loss induction, were measured by quantitative RT-PCR after Houttuyniae Herbtreatment. Houttuyniae Herb did not significantly affect mRNA expression of BMP6, FGF7, FGF10, and β-catenin, which are important factors for regulating the hair cycle, including type 1 5α-reductase. However, mRNA expression of type 2 5α-reductase, the major cause of male hair loss, was significantly reduced to 56.1% by treatment of Houttuyniae Herbtreatment. Taken together, these results suggest that the Houttuyniae Herbtreatment can help to treat lair loss through removing free radicals and suppression of the expression level of type 2 5α-reductase in DP cells.
Abstract
Houttuyniae Herba is widely used as a cosmetic for enhancing hair growth, and study on promoting mouse hair growth has also been reported. However, studies on the effects of the Houttuyniae Herba on dermal papilla (DP) cells, which play an important role in hair growth, are not well known. For this reason, we studied the effect of Houttuyniae Herba on DP cells. The strong antioxidant activity of Houttuyniae Herba was confirmed by ABTS assay. In the MTS assay, cell viability was reduced to 94.5% in DP cells by treatment of 2 mg/ml concentration of Houttuyniae Herb and cytotoxicity was not observed at 1 mg/ml concentration. The mRNA expression levels of Bone morphogenetic pretein (BMP6), fibroblast growth factor 7 (FGF7), FGF10, and β-galactosidase genes, which are involved in hair growth cycle and hair loss induction, were measured by quantitative RT-PCR after Houttuyniae Herbtreatment. Houttuyniae Herb did not significantly affect mRNA expression of BMP6, FGF7, FGF10, and β-catenin, which are important factors for regulating the hair cycle, including type 1 5α-reductase. However, mRNA expression of type 2 5α-reductase, the major cause of male hair loss, was significantly reduced to 56.1% by treatment of Houttuyniae Herbtreatment. Taken together, these results suggest that the Houttuyniae Herbtreatment can help to treat lair loss through removing free radicals and suppression of the expression level of type 2 5α-reductase in DP cells.
참고문헌 (32)
- Tao HJ, "Mingyibielu", pp.321~ (1986), Renminweishengchubanshe
- Korea Food and Drug Administration, "The Korean Herbal Pharmacopoeia", pp.275~277 (2017), Korea Food and Drug Administration
- Kim IR, "Boncho-Hak", pp.270~271 (2007), Young-Lim Press
- Miao MS, "Modern practical chinese traditional medicine quality control technology", pp.43~ (2000), People's Hygiene Press
- 허유진, "Houttuynia cordata Improves Cognitive Deficits in Cholinergic Dysfunction Alzheimer’s Disease-Like Models", Biomolecules & Therapeutics, 22(3), pp.176~183 (2014), 한국응용약물학회
- Rho BG, "Studies on the antiallergic reactions of the Herba Houttuyniae extract", Kor J Herbology, 13(2), pp.77~89 (1998)
- Kim SK, "Cytotoxic alkaloids from Houttuynia cordata", Archives of Pharmacal Research, 24(6), pp.518~521 (2001)
- 정희록, "어성초 추출물의 항산화 및 신경세포 보호효과", 한국식품저장유통학회지, 17(5), pp.720~726 (2010), 한국식품저장유통학회
- 김동혁, "어성초 ethanol 추출물의 마우스 살모넬라 감염증에 대한 항균 및 치료효과 규명", 한국환경농학회지, 27(2), pp.156~162 (2008), 한국환경농학회
- Kwak IS, "NEW Dupimobal gwanli-Hak", pp.21~22 (2011), Gun-Ja Press
- OOh JW, "A guide to studying human hair follicle cycling in vivo", J Invest Dermatol, 136(1), pp.34~44 (2016)
- Jang, Hae-Young, "Bibliographic Studies of Depilation", Journal of Pharmacopuncture, 5(2), pp.92~108 (2002), 대한약침학회[DOI]
- Yim SBN, "A literature study about the comparison of Oriental-Occidental medicine on the alopecia", The Journal of Jehan oriental medical academy, 4(1), pp.699~710 (1999)
- 홍양희, "어성초를 포함한 천연복합추출물의 C57BL", 6 마우스에 대한 발모효과, 2015, pp.13(3), 321~329[DOI]
- Kim CD, "Identification of androgen-regulated genes in SV40-transformed human hair dermal papilla cells", J Dermatol Sci, 32(2), pp.143~149 (2003)
- Trüeb RM, "The impact of oxidative stress on hair", Int J Cosmet Sci, 37(Suppl 2), pp.25~30 (2015)
- Solanas G, "Regenerating the skin : a task for the heterogeneous stem cell pool and surrounding niche", Nat Rev Mol Cell Biol, 14(11), pp.737~748 (2013)
- Enshell-Seijffers D, "Beta-catenin activity in the dermal papilla regulates morphogenesis and regeneration of hair", Dev Cell, 18(4), pp.633~642 (2010)
- Lei MX, "Tuning Wnt Signals for More or Fewer Hairs", J Invest Dermatol, 133(1), pp.7~9 (2013)
- Kim HC., "Hanbangyagli-Hak", pp.165~166 (2001), Jibmundang
- Meng J, "Study on chemical constituents of flavonoids in fresh herb of Houttuynia cordata", Zhongguo Zhong Yao Za Zhi, 31(16), pp.1335~1337 (2006)
- Lu HM, "Variation in chemical composition and antibacterial activities of essential oils from two species of Houttuynia Thunb", Chem. Pharm. Bull, 54(7), pp.936~940 (2006)
- Kim SK, "Cytotoxic alkaloids from Houttuynia cordata", Arch. Pharm. Res, 24(6), pp.518~521 (2001)
- Peters EM, "Hair growth inhibition by psychoemotional stress: a mouse model for neural mechanisms in hair growth control", Exp. Dermatol, 15(1), pp.1~13 (2006)
- Trüeb RM, "Molecular mechanisms of androgenetic alopecia", Experimental Gerontology, 37(8-9), pp.981~990 (2002)
- Wei YH, "Oxidative stress, mitochondrial DNA mutation, and impairment of antioxidant enzymes in aging", Exp Biol Med, 227(9), pp.671~682 (2002)
- Naito A, "Lipid peroxides induce early onset of catagen phase in murine hair cycles", Int J Mol Med, 22(6), pp.725~729 (2008)
- Liu N, "Chronic Restraint Stress Inhibits Hair Growth via Substance P Mediated by Reactive Oxygen Species in Mice", PLoS One, 8(4), pp.e61574~ (2013)
- Rahmani W, "Hair Follicle Dermal Stem Cells Regenerate the Dermal Sheath, Repopulate the Dermal Papilla, and Modulate Hair Type", Dev Cell, 31(5), pp.543~558 (2014)
- Liu S, "Different patterns of 5alpha-reductase expression, cellular distribution, and testosterone metabolism in human follicular dermal papilla cells", Biochem Biophys Res Commun, 368(4), pp.858~864 (2008)
- Asada Y, "5 alpha-reductase type 2 is constitutively expressed in the dermal papilla and connective tissue sheath of the hair follicle in vivo but not during culture in vitro", J Clin Endocrinol Metab, 86(6), pp.2875~2880 (2001)
- Kaufman KD, "Finasteride in the treatment of men with androgenetic alopecia. Finasteride Male Pattern Hair Loss Study Group", J Am Acad Dermatol, 39(4Pt1), pp.578~589 (1998)