탈모 증상 완화 및 육모 화장품 소재로써 Derma GenieTM-H001의 생리활성 효능 평가
Evaluation of Derma Genie™-H001 as a Functional Cosmetic Ingredient for Hair Loss Improvement: Assessing Anti-inflammatory, Antioxidative, Photoprotective, Antiandrogenic, and Hair Growth-Inductive Effects
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초록
목적: 본 연구는 Derma GenieTM-H001을 이용하여 세포 내 항산화, 항염, 광보호, 항안드로겐 및 모발 성장과 연관된 유전자 발현 변화 등을 평가하여 탈모 증상 완화를 위한 기능성 화장품 원료로의 Derma GenieTM의 적용 가능성을 입증하였다. 방법: 항염 효능을 평가하기 위해서 NO assay를, 염증성 사이토카인의 유전자 발현 분석을 위해서는 qRT-PCR을 수행하였다. 모유두 세포 내 항산화 작용 및 광 보호 효능을 확인하기 위해서 H2O2 처리 및 UVB가 조사된 모유두 세포에서 WST-1 assay, DCF-DA assay, LDH leakage assay, UVB-irradiation assay 등이 시행되었다. 또한 모유두세포 내 모발 성장과 연관된 유전자 발현 변화를 확인하기 위해 qRT-PCR을 수행하였으며, 성장기 모발의 유도 가능성을 확인하였다. 결과: Derma GenieTM-H001에서 폴리페놀류 화합물이 함유되어 있고, DPPH radical 소거능이 있음을 확인하였다. 2D monolayer 배양에서는 모유두세포에서 독성을 보이지 않고 세포 증식률을 증가시켰으며, 3D spheroid 배양에서 모유두세포 구형 크기를 증가시키는 것을 확인하였다. 염증성 인자인 산화 질소와 3종의 염증성 사이토 카인(TNF-α, IL-1β, NOS2)은 Derma GenieTM가 처리된 Raw 264.7 세포에서 현저하게 감소되었다. 또한 H2O2 처리 및 UVB가 조사된 모유두세포에서 Derma GenieTM가 상당한 세포 독성 완화 효능을 가지는 것을 확인했다. 마지막으로, qRT-PCR 분석을 통해 모발 퇴행을 야기하는 유전자 발현(AR, TGF-β1)은 감소시키고, 모발 성장에 필수적인 유전자 발현(ALPL, LEF1, VCAN, FGF2, FGF7, FGF10, WNT5A, VEGF)은 Derma Genie™ 처리군에서 농도 의존적으로 증가되는 것을 확인하였다. 결론: 이러한 연구 결과는 Derma Genie™-H001이 세포 내에서 항염, 항산화, 항안드로겐, 광보호 작용 능력이 우수하였으며, 모발 성장 개시를 위한 다양한 유전자의 발현 효과가 높게 나타나 탈모 완화 및 육모 기능성 화장품의 천연 소재로의 적용 가능성을 확인하였다.
Abstract
Purpose: This study aimed to assess the potential of Derma Genie™-H001 as a functional cosmetic ingredient for alleviating hair loss symptoms by evaluating its impact on anti-inflammatory, antioxidative, photoprotective, antiandrogenicity, and activation of hair growth-related gene expression. Methods: We employed various methods, including nitric oxide (NO) assay and quantitative real-time polymerase chain reaction (qRT-PCR, to estimate anti-inflammatory activities). Additionally, this study assessed antioxidative and photoprotective effects on human dermal papilla cells (HDPCs) using water-soluble tetrazolium salt (WST-1) assay, 2′,7′-dichlorofluorescin diacetate (DCF-DA) assay, lactate dehydrogenase (LDH) assay, and ultraviolet B (UVB)-irradiation assay. To investigate the anti-hair loss effects of Derma Genie™-H001, 3D spheroid culture and qRT-PCR were conducted. Results: Derma Genie™-H001 exhibited the characteristics of polyphenols and demonstrated 2,2-dipheny-1-picrylhydrazyl (DPPH) radical scavenging activity. Importantly, it showed no cytotoxicity on HDPCs and increased the diameter of HDPCs 3D spheroid. Inflammatory mediators such as NO and proinflammatory cytokines were reduced in a dose-dependent manner in Raw 264.7 cells treated with Derma Genie™. Moreover, Derma Genie™ exhibited substantial protective effects against hydrogen peroxide (H2O2) and UVB-induced cytotoxicity in HDPCs. Notably, qRT-PCR analysis revealed a decrease in the expression of genes related to hair loss and an increase in the expression of genes associated with hair growth in HDPCs treated with Derma Genie™. Conclusion: Derma Genie™-H001 demonstrated promising effects in mitigating LPS-induced proinflammatory cytokines, H2O2-induced cytotoxicity, UVB-induced cytotoxicity, and DHT-induced TGF-β1 expression. Furthermore, it significantly upregulated the expression of hair growth-related genes. These findings suggest that Derma Genie™ may serve as a valuable functional cosmetic ingredient for improving hair loss symptoms.
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