음경해면체에 미치는 山茱萸 물 추출물의 이완효과
Relaxation Effects of Corni Fructus Water Extract on Corpus Cavernosum
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To investigate the effect of Corni Fructus(CF) water extracts on the relaxation of the corpus cavernosum, organ bath studies, histochemical and immunohistochemical methods were used and obtained the following results. In the organ bath study, the maximal contraction of the corpus cavernosum tissue by PE showed a significant relaxation effect at 3.0 ㎎/㎖ of CF water extract. When L-NNA was pretreated corpuscular relaxation effect of CF water extract was significantly inhibited compared without L-NNA pretreatment. In Ca2+-free solution, the increase of contraction due to Ca2+ influx significantly inhibited in the pretreatment compared with no pretreatment of CF water extract. Histochemical and immunohistochemical studies showed that the ratio of smooth muscle to collagen fiber was increased in the CF group compared to the PE group in the corpus cavernosum, and the eNOS positive reaction increased and the PDE5 positive reaction decreased. These results suggest that CF extract has increased NO production through activation of eNOS and inhibited the action of PDE5 to block the extracellular Ca2+ influx, thereby relaxing the smooth muscle of the corpus cavernosum.
Abstract
To investigate the effect of Corni Fructus(CF) water extracts on the relaxation of the corpus cavernosum, organ bath studies, histochemical and immunohistochemical methods were used and obtained the following results. In the organ bath study, the maximal contraction of the corpus cavernosum tissue by PE showed a significant relaxation effect at 3.0 ㎎/㎖ of CF water extract. When L-NNA was pretreated corpuscular relaxation effect of CF water extract was significantly inhibited compared without L-NNA pretreatment. In Ca2+-free solution, the increase of contraction due to Ca2+ influx significantly inhibited in the pretreatment compared with no pretreatment of CF water extract. Histochemical and immunohistochemical studies showed that the ratio of smooth muscle to collagen fiber was increased in the CF group compared to the PE group in the corpus cavernosum, and the eNOS positive reaction increased and the PDE5 positive reaction decreased. These results suggest that CF extract has increased NO production through activation of eNOS and inhibited the action of PDE5 to block the extracellular Ca2+ influx, thereby relaxing the smooth muscle of the corpus cavernosum.
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